使用化学诱导的小鼠卵巢衰竭模型研究卵巢衰竭对单个肌纤维收缩力的时间影响。

IF 2
Parastoo Mashouri, Jinan Saboune, Glen Pyle, Geoffrey Alonzo Power
{"title":"使用化学诱导的小鼠卵巢衰竭模型研究卵巢衰竭对单个肌纤维收缩力的时间影响。","authors":"Parastoo Mashouri, Jinan Saboune, Glen Pyle, Geoffrey Alonzo Power","doi":"10.1139/apnm-2025-0130","DOIUrl":null,"url":null,"abstract":"<p><p>We investigated the effects of chemically-induced ovarian failure on single fibre contractility of the soleus and extensor digitorum longus (EDL) muscles throughout ovarian failure, thereby mimicking the menopausal transition into late-stage menopause: [(D60;peri-menopause), (D120;onset of menopause), (D134;early-onset menopause), (D176;late-stage menopause)]. We used 4-vinylcyclohexene diepoxide (VCD) to induce ovarian failure in sexually-mature female mice. For the soleus at D120 and D176, mice with VCD-induced ovarian failure produced higher force as compared with controls (p<0.05). On D134, however, VCD had lower force production compared with controls (p<0.05). The cross-sectional area of the soleus fibres from the VCD group was larger at D120 compared with controls (p<0.05), but not at any other time point (p>0.05). The VCD group showed time-dependent changes in specific force production compared to controls, with a 37% decrease at D134 but a 39% increase at D176 in the soleus muscle (p<0.05). No differences in rate of force redevelopment (Ktr) or calcium sensitivity was observed for the soleus (p>0.05). For the EDL there were no differences in force, cross-sectional area, stiffness, Ktr, or calcium sensitivity between groups (p>0.05). Our results indicate that muscle contractility across the peri-menopausal transition into late-stage menopause is both muscle and phase-dependent, emphasizing the complexity of changing hormones throughout the lifespan on muscle contractile function.</p>","PeriodicalId":93878,"journal":{"name":"Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme","volume":" ","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Investigating the temporal effects of ovarian failure on single muscle fibre contractility using a chemically-induced ovarian failure model in mice.\",\"authors\":\"Parastoo Mashouri, Jinan Saboune, Glen Pyle, Geoffrey Alonzo Power\",\"doi\":\"10.1139/apnm-2025-0130\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>We investigated the effects of chemically-induced ovarian failure on single fibre contractility of the soleus and extensor digitorum longus (EDL) muscles throughout ovarian failure, thereby mimicking the menopausal transition into late-stage menopause: [(D60;peri-menopause), (D120;onset of menopause), (D134;early-onset menopause), (D176;late-stage menopause)]. We used 4-vinylcyclohexene diepoxide (VCD) to induce ovarian failure in sexually-mature female mice. For the soleus at D120 and D176, mice with VCD-induced ovarian failure produced higher force as compared with controls (p<0.05). On D134, however, VCD had lower force production compared with controls (p<0.05). The cross-sectional area of the soleus fibres from the VCD group was larger at D120 compared with controls (p<0.05), but not at any other time point (p>0.05). The VCD group showed time-dependent changes in specific force production compared to controls, with a 37% decrease at D134 but a 39% increase at D176 in the soleus muscle (p<0.05). No differences in rate of force redevelopment (Ktr) or calcium sensitivity was observed for the soleus (p>0.05). For the EDL there were no differences in force, cross-sectional area, stiffness, Ktr, or calcium sensitivity between groups (p>0.05). Our results indicate that muscle contractility across the peri-menopausal transition into late-stage menopause is both muscle and phase-dependent, emphasizing the complexity of changing hormones throughout the lifespan on muscle contractile function.</p>\",\"PeriodicalId\":93878,\"journal\":{\"name\":\"Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-09-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1139/apnm-2025-0130\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1139/apnm-2025-0130","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

我们研究了化学诱导的卵巢衰竭对整个卵巢衰竭过程中比目鱼肌和指长伸肌(EDL)单纤维收缩力的影响,从而模拟了更年期过渡到晚期更年期的过程:[(D60;围绝经期),(D120;更年期开始),(D134;早发性更年期),(D176;更年期晚期)]。我们用4-乙烯基二氧化二环己烯(VCD)诱导性成熟雌性小鼠卵巢衰竭。对于D120和D176时的比目鱼肌,vcd诱导的卵巢衰竭小鼠比对照组产生更高的力(p0.05)。与对照组相比,VCD组在比目鱼肌的比力产生方面显示出随时间的变化,在D134时下降37%,而在D176时增加39% (p0.05)。EDL的受力、横截面积、刚度、Ktr、钙敏感性各组间无差异(p < 0.05)。我们的研究结果表明,从围绝经期过渡到绝经后期,肌肉收缩力既依赖于肌肉,也依赖于阶段,强调了整个生命周期中激素变化对肌肉收缩功能的复杂性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigating the temporal effects of ovarian failure on single muscle fibre contractility using a chemically-induced ovarian failure model in mice.

We investigated the effects of chemically-induced ovarian failure on single fibre contractility of the soleus and extensor digitorum longus (EDL) muscles throughout ovarian failure, thereby mimicking the menopausal transition into late-stage menopause: [(D60;peri-menopause), (D120;onset of menopause), (D134;early-onset menopause), (D176;late-stage menopause)]. We used 4-vinylcyclohexene diepoxide (VCD) to induce ovarian failure in sexually-mature female mice. For the soleus at D120 and D176, mice with VCD-induced ovarian failure produced higher force as compared with controls (p<0.05). On D134, however, VCD had lower force production compared with controls (p<0.05). The cross-sectional area of the soleus fibres from the VCD group was larger at D120 compared with controls (p<0.05), but not at any other time point (p>0.05). The VCD group showed time-dependent changes in specific force production compared to controls, with a 37% decrease at D134 but a 39% increase at D176 in the soleus muscle (p<0.05). No differences in rate of force redevelopment (Ktr) or calcium sensitivity was observed for the soleus (p>0.05). For the EDL there were no differences in force, cross-sectional area, stiffness, Ktr, or calcium sensitivity between groups (p>0.05). Our results indicate that muscle contractility across the peri-menopausal transition into late-stage menopause is both muscle and phase-dependent, emphasizing the complexity of changing hormones throughout the lifespan on muscle contractile function.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信