Parastoo Mashouri, Jinan Saboune, Glen Pyle, Geoffrey Alonzo Power
{"title":"使用化学诱导的小鼠卵巢衰竭模型研究卵巢衰竭对单个肌纤维收缩力的时间影响。","authors":"Parastoo Mashouri, Jinan Saboune, Glen Pyle, Geoffrey Alonzo Power","doi":"10.1139/apnm-2025-0130","DOIUrl":null,"url":null,"abstract":"<p><p>We investigated the effects of chemically-induced ovarian failure on single fibre contractility of the soleus and extensor digitorum longus (EDL) muscles throughout ovarian failure, thereby mimicking the menopausal transition into late-stage menopause: [(D60;peri-menopause), (D120;onset of menopause), (D134;early-onset menopause), (D176;late-stage menopause)]. We used 4-vinylcyclohexene diepoxide (VCD) to induce ovarian failure in sexually-mature female mice. For the soleus at D120 and D176, mice with VCD-induced ovarian failure produced higher force as compared with controls (p<0.05). On D134, however, VCD had lower force production compared with controls (p<0.05). The cross-sectional area of the soleus fibres from the VCD group was larger at D120 compared with controls (p<0.05), but not at any other time point (p>0.05). The VCD group showed time-dependent changes in specific force production compared to controls, with a 37% decrease at D134 but a 39% increase at D176 in the soleus muscle (p<0.05). No differences in rate of force redevelopment (Ktr) or calcium sensitivity was observed for the soleus (p>0.05). For the EDL there were no differences in force, cross-sectional area, stiffness, Ktr, or calcium sensitivity between groups (p>0.05). Our results indicate that muscle contractility across the peri-menopausal transition into late-stage menopause is both muscle and phase-dependent, emphasizing the complexity of changing hormones throughout the lifespan on muscle contractile function.</p>","PeriodicalId":93878,"journal":{"name":"Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme","volume":" ","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Investigating the temporal effects of ovarian failure on single muscle fibre contractility using a chemically-induced ovarian failure model in mice.\",\"authors\":\"Parastoo Mashouri, Jinan Saboune, Glen Pyle, Geoffrey Alonzo Power\",\"doi\":\"10.1139/apnm-2025-0130\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>We investigated the effects of chemically-induced ovarian failure on single fibre contractility of the soleus and extensor digitorum longus (EDL) muscles throughout ovarian failure, thereby mimicking the menopausal transition into late-stage menopause: [(D60;peri-menopause), (D120;onset of menopause), (D134;early-onset menopause), (D176;late-stage menopause)]. We used 4-vinylcyclohexene diepoxide (VCD) to induce ovarian failure in sexually-mature female mice. For the soleus at D120 and D176, mice with VCD-induced ovarian failure produced higher force as compared with controls (p<0.05). On D134, however, VCD had lower force production compared with controls (p<0.05). The cross-sectional area of the soleus fibres from the VCD group was larger at D120 compared with controls (p<0.05), but not at any other time point (p>0.05). The VCD group showed time-dependent changes in specific force production compared to controls, with a 37% decrease at D134 but a 39% increase at D176 in the soleus muscle (p<0.05). No differences in rate of force redevelopment (Ktr) or calcium sensitivity was observed for the soleus (p>0.05). For the EDL there were no differences in force, cross-sectional area, stiffness, Ktr, or calcium sensitivity between groups (p>0.05). Our results indicate that muscle contractility across the peri-menopausal transition into late-stage menopause is both muscle and phase-dependent, emphasizing the complexity of changing hormones throughout the lifespan on muscle contractile function.</p>\",\"PeriodicalId\":93878,\"journal\":{\"name\":\"Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-09-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1139/apnm-2025-0130\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1139/apnm-2025-0130","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Investigating the temporal effects of ovarian failure on single muscle fibre contractility using a chemically-induced ovarian failure model in mice.
We investigated the effects of chemically-induced ovarian failure on single fibre contractility of the soleus and extensor digitorum longus (EDL) muscles throughout ovarian failure, thereby mimicking the menopausal transition into late-stage menopause: [(D60;peri-menopause), (D120;onset of menopause), (D134;early-onset menopause), (D176;late-stage menopause)]. We used 4-vinylcyclohexene diepoxide (VCD) to induce ovarian failure in sexually-mature female mice. For the soleus at D120 and D176, mice with VCD-induced ovarian failure produced higher force as compared with controls (p<0.05). On D134, however, VCD had lower force production compared with controls (p<0.05). The cross-sectional area of the soleus fibres from the VCD group was larger at D120 compared with controls (p<0.05), but not at any other time point (p>0.05). The VCD group showed time-dependent changes in specific force production compared to controls, with a 37% decrease at D134 but a 39% increase at D176 in the soleus muscle (p<0.05). No differences in rate of force redevelopment (Ktr) or calcium sensitivity was observed for the soleus (p>0.05). For the EDL there were no differences in force, cross-sectional area, stiffness, Ktr, or calcium sensitivity between groups (p>0.05). Our results indicate that muscle contractility across the peri-menopausal transition into late-stage menopause is both muscle and phase-dependent, emphasizing the complexity of changing hormones throughout the lifespan on muscle contractile function.