利用光纤机械共振探针无标记检测早期蛋白质聚集。

IF 3.3 2区 物理与天体物理 Q2 OPTICS
Optics express Pub Date : 2025-09-08 DOI:10.1364/OE.571612
Yuliang Liu, Guifeng Li, Guoquan Wang, Zhonghao Li, Chaoyang Gong, Tao Zhu
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引用次数: 0

摘要

蛋白质聚集体的早期检测为各种神经退行性疾病的病理生理学提供了有价值的见解。然而,作为检测蛋白质聚集的“黄金标准”的荧光方法依赖于与聚集的蛋白质结合的荧光团,这可能会干扰聚集过程。在这里,我们提出了一种光纤机械共振探针(FOMRP),通过监测蛋白质溶液的力学性质,用于无标记检测早期蛋白质聚集。该材料采用锥形光纤制成,由压电驱动器驱动。采用光纤光栅对复合材料的机械振动进行了监测。我们发现,FOMRP支持一系列非线性机械振动。我们研究了基振和简振模式之间的能量传递机制。发现1/2阶次谐波振动对外部环境高度敏感,随后将其用于粘度测量。进一步应用于监测早期蛋白质聚集,显示出比传统荧光法更高的灵敏度。这种方法为无标记蛋白质聚集监测提供了一种有前途的工具,并在分子和细胞力学分析中具有潜在的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Label-free detection of early-stage protein aggregation using a fiber-optic mechanical resonant probe.

Early detection of protein aggregates offers valuable insights into the pathophysiology of a variety of neurodegenerative diseases. However, fluorescence methods, which serve as the "golden standard" for detecting protein aggregation, rely on fluorophores that bind to aggregated proteins, potentially interfering with the aggregation process. Here, we propose a fiber-optic mechanical resonant probe (FOMRP) for the label-free detection of early-stage protein aggregation by monitoring the mechanical properties of the protein solution. The FOMRP was fabricated by a tapered optical fiber and was driven by a piezoelectric actuator. The mechanical vibration of FOMRP was monitored with a fiber Bragg grating. We found that the FOMRP supports a series of nonlinear mechanical vibrations. We investigated the energy transfer mechanism between the fundamental and harmonic vibration modes. The 1/2 order subharmonic vibration was found to be highly sensitive to the external environment and was subsequently employed for viscosity measurement. The FOMRP was further applied to monitor early-stage protein aggregation, showing higher sensitivity than the conventional fluorescence method. This approach provides a promising tool for label-free protein aggregation monitoring and has potential applications in molecular and cellular mechano-assays.

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来源期刊
Optics express
Optics express 物理-光学
CiteScore
6.60
自引率
15.80%
发文量
5182
审稿时长
2.1 months
期刊介绍: Optics Express is the all-electronic, open access journal for optics providing rapid publication for peer-reviewed articles that emphasize scientific and technology innovations in all aspects of optics and photonics.
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