用于高灵敏度检测阿尔茨海默病诊断靶点MMP10的光纤表面等离子体共振传感器。

IF 3.3 2区 物理与天体物理 Q2 OPTICS
Optics express Pub Date : 2025-09-08 DOI:10.1364/OE.573237
Yong Wei, Yuye Xuan, Chunlan Liu, Xiaoshan Li, Yonghui Zhang, Chenling Shi, Songquan Li, Qian Yang, Zhihai Liu
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引用次数: 0

摘要

现有的阿尔茨海默病(Alzheimer's disease, AD)诊断靶点的体内检测方法存在无法实时检测和对配体生物相容性识别能力差两个问题,难以有效实现对AD的早期快速筛查。本文提出了一种具有表面修饰槲皮素配体的光纤表面等离子体共振(SPR)传感器,该传感器成功检测了人工脑脊液中我们认为是新的早期AD诊断靶点基质金属蛋白酶-10 (MMP10)。具有实时检测、生物相容性好等特点。通过分子对接技术模拟槲皮素与MMP10的分子结合机理,证实两者的结合自由能高达-9.0 kcal/mol。采用塑料包覆的阶跃指数多模光纤构建槲皮素功能化反射式SPR传感器,将其插入医用针头中,在蛛网膜下腔模拟脑脊液环境中实现MMP10的浓度检测。实验数据表明,该传感器的检测灵敏度为7.06 nm/lg (pg/mL),检出限为1.41 pg/mL,符合临床检测标准,且识别配体对人体无害。这有望用于实时检测体内AD早期诊断靶点,为AD的早期快速筛查提供新的思路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fiber optic surface plasmon resonance sensor for high-sensitivity detection of MMP10, a diagnostic target for Alzheimer's disease.

The existing in vivo detection methods for Alzheimer's disease (AD) diagnostic targets have two problems of inability to detect in real time and poor recognition of ligand biocompatibility, making it difficult to effectively achieve early and rapid screening for AD. This article proposes a fiber optic surface plasmon resonance (SPR) sensor with a surface-modified quercetin ligand, which successfully detects what we believe to be a novel early AD diagnostic target matrix metalloproteinase-10 (MMP10) in artificial cerebrospinal fluid. It has the characteristics of real-time detection and good biocompatibility. By using molecular docking technology to simulate the molecular binding mechanism between quercetin and MMP10, it was confirmed that the binding free energy of the two was as high as -9.0 kcal/mol. A reflective SPR sensor functionalized with quercetin was constructed using a plastic-coated step-index multimode fiber, which was inserted into a medical needle to achieve concentration detection of MMP10 in a simulated cerebrospinal fluid environment in the subarachnoid space. Experimental data indicate that the detection sensitivity of the sensor is 7.06 nm/lg (pg/mL), with a detection limit of 1.41 pg/mL, which meets the clinical detection standards, and the recognition ligand is harmless to the human body. This is expected to be used for real-time detection of early AD diagnostic targets in vivo, providing new ideas for early and rapid screening of AD.

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来源期刊
Optics express
Optics express 物理-光学
CiteScore
6.60
自引率
15.80%
发文量
5182
审稿时长
2.1 months
期刊介绍: Optics Express is the all-electronic, open access journal for optics providing rapid publication for peer-reviewed articles that emphasize scientific and technology innovations in all aspects of optics and photonics.
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