液相色谱-高分辨率质谱法检测半固体剂型蜂毒的特征肽蜂毒素

IF 1.1 4区化学 Q4 CHEMISTRY, ANALYTICAL

Journal of Analytical Chemistry Pub Date : 2025-09-23 DOI:10.1134/S1061934825700789

M. F. Vokuev, A. Yu. Oprunenko, A. V. Frolova, T. M. Baygildiev, I. A. Rodin

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引用次数: 0

摘要

由于其药理和生物活性，蜂毒被用于生产各种药物和药物形式，包括软膏和乳霜。蜂毒素是蜂毒的主要成分，它在药物制剂中的存在是蜂毒作为成分存在的特征。提出了一种检测半固体剂型蜂毒中这种特征肽成分的方法，该方法包括使用固相萃取法回收和预浓缩，然后使用液相色谱法和质谱法检测。验证了检测方法的特异性，检出限为0.1 μg/g。

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Detection of Melittin, a Characteristic Peptide of Bee Venom, in Semisolid Dosage Forms Using Liquid Chromatography–High-Resolution Mass Spectrometry

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Detection of Melittin, a Characteristic Peptide of Bee Venom, in Semisolid Dosage Forms Using Liquid Chromatography–High-Resolution Mass Spectrometry

Due to its pharmacological and biological activity, bee venom is used in the production of various drugs and pharmaceutical forms, including ointments and creams. Melittin is the main component of bee venom, and its presence in a drug preparation is characteristic of the presence of bee venom as a constituent. An approach to the detection of this characteristic peptide component of bee venom in semisolid dosage forms, which involves recovery and preconcentration using solid-phase extraction followed by liquid chromatography with mass spectrometric detection, was proposed. The specificity of the detection procedure was validated, and the limit of detection was 0.1 μg/g.

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来源期刊

Journal of Analytical Chemistry 化学-分析化学

CiteScore

2.10

自引率

9.10%

发文量

146

审稿时长

13 months

期刊介绍： The Journal of Analytical Chemistry is an international peer reviewed journal that covers theoretical and applied aspects of analytical chemistry; it informs the reader about new achievements in analytical methods, instruments and reagents. Ample space is devoted to problems arising in the analysis of vital media such as water and air. Consideration is given to the detection and determination of metal ions, anions, and various organic substances. The journal welcomes manuscripts from all countries in the English or Russian language.