急性高海拔暴露时视网膜小胶质细胞的激活和迁移

IF 2.7 2区 医学 Q1 OPHTHALMOLOGY
Yuting Li , Cong Han , Jianping Zhang , Songjian Huang , Xin Zhao , Jiarui Zhu , Qian Niu , Yi Yang , Wenfang Zhang
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引用次数: 0

摘要

高原视网膜病变(High-altitude retinopathy, HAR)是由低气压缺氧引起的视网膜功能障碍。但其发病机制尚不明确。小胶质细胞是视网膜上的常驻免疫细胞,在各种视网膜疾病中起着至关重要的作用。在这里,我们研究了暴露于高海拔(5000米HAE)的小鼠模型中小胶质细胞激活的功能改变和潜在机制。苏木精和伊红(H&;E)染色显示高海拔暴露(HAE)后视网膜全层、内层和外层水肿。视网膜电图(ERG)检测显示,在低气压缺氧条件下,视网膜功能受损。免疫荧光染色证实了HAE后视网膜内小胶质细胞数量的时间依赖性增加,激活的小胶质细胞在持续缺氧损伤期间迁移。此外,这些被激活的小胶质细胞在急性高海拔条件下主要分化为促炎亚型。值得注意的是,在HAE后不同时间点,Connexin43 (Cx43)免疫反应性增加,而白细胞介素-1β (IL-1β)水平显著升高。在本研究中,我们证实了小胶质细胞的激活和迁移参与了低气压缺氧引起的视网膜水肿和功能损伤。此外,急性HAE下Cx43的动态变化和IL-1β的上调可能与小胶质细胞的炎症激活有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Microglia activation and migration in retina during acute high-altitude exposure

Microglia activation and migration in retina during acute high-altitude exposure
High-altitude retinopathy (HAR), caused by hypobaric hypoxia, leads to retinal dysfunction. However, its pathogenic mechanism remains elusive. Microglia, the resident immune cells of the retina, play crucial roles in various retinal disorders. Here, we investigated the functional alterations and underlying mechanisms of microglial activation in mouse models exposed to high-altitude (5000m HAE). Hematoxylin and eosin (H&E) staining showed edema in the entire, inner, and outer retinal layers after high-altitude exposure (HAE). Electroretinogram (ERG) testing revealed impaired retinal function under hypobaric hypoxia. Immunofluorescence staining confirmed a time-dependent increase in microglial numbers within the retina following HAE, with activated microglia migrating during persistent hypoxic injury. Furthermore, these activated microglia predominantly differentiated into pro-inflammatory subtypes under acute high-altitude conditions. Notably, Connexin43 (Cx43) immunoreactivity increased, while interleukin-1β (IL-1β) levels were markedly elevated at different time points after HAE. In this study, we confirmed that microglia activation and migration are involved in retinal edema and functional injury induced by hypobaric hypoxia. Additionally, the dynamic changes in Cx43 and up-regulation of IL-1β might be related to the inflammatory activation of microglia under acute HAE.
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来源期刊
Experimental eye research
Experimental eye research 医学-眼科学
CiteScore
6.80
自引率
5.90%
发文量
323
审稿时长
66 days
期刊介绍: The primary goal of Experimental Eye Research is to publish original research papers on all aspects of experimental biology of the eye and ocular tissues that seek to define the mechanisms of normal function and/or disease. Studies of ocular tissues that encompass the disciplines of cell biology, developmental biology, genetics, molecular biology, physiology, biochemistry, biophysics, immunology or microbiology are most welcomed. Manuscripts that are purely clinical or in a surgical area of ophthalmology are not appropriate for submission to Experimental Eye Research and if received will be returned without review.
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