Kaiqiang Li , Jiajiang Lin , Gary Owens , Zuliang Chen
{"title":"高岭土生物浸出中的巴氏梭菌:铁还原和增白机制","authors":"Kaiqiang Li , Jiajiang Lin , Gary Owens , Zuliang Chen","doi":"10.1016/j.clay.2025.107998","DOIUrl":null,"url":null,"abstract":"<div><div>Previous studies have shown that microbial consortia outperform single strains in bioleaching processes. However, gaps remain in understanding the microbial community structure and functional interactions between key species and critical metabolic genes. This study addressed these questions through iron-reducing microbe-mediated bioleaching experiments combined with multi-omic approaches. The study found four major findings: (1) Bioleaching significantly enhanced the whiteness of kaolin from 60.8 to 90.4 % by selectively removing Fe (III), reducing the Fe (III) content from 1.5 % to 1.2 % without altering the kaolin structure. This process was accompanied by the solubilization of Fe (II). (2) Community succession identified <em>Clostridium</em> (87.7 %), <em>Lysinibacillus</em> (5.9 %), and <em>Bacillus</em> (0.7 %) as the dominant populations. Spearman analysis confirmed strong positive correlations between <em>Clostridium</em> abundance, Fe<sup>2+</sup> concentration, and whiteness (<em>p</em> < 0.05), suggesting that together with Fe (III) content in kaolin <em>Clostridium</em> plays a key role in whitening. (3) Metagenomic reconstruction revealed <em>Clostridium pasteurianum</em> (50.8 %) was the core functional species. Its complete glycolysis/pyruvate metabolism pathways and acidogenesis gene clusters (lactic/formic/butyric acids) synergistically facilitated Fe (III) dissolution. Notably, this strain exhibited enriched ferredoxin-coding genes and membrane-bound electron transport chain components, suggesting it had a pivotal role in dissimilatory iron reduction. This work provides the first elucidation of structure-function relationships and metabolic networks within iron-reducing consortia, offering both theoretical foundations and practical strategies for sustainable mineral bio-processing.</div></div>","PeriodicalId":245,"journal":{"name":"Applied Clay Science","volume":"278 ","pages":"Article 107998"},"PeriodicalIF":5.8000,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Clostridium pasteurianum in kaolin bioleaching: Iron reduction and whitening mechanisms\",\"authors\":\"Kaiqiang Li , Jiajiang Lin , Gary Owens , Zuliang Chen\",\"doi\":\"10.1016/j.clay.2025.107998\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Previous studies have shown that microbial consortia outperform single strains in bioleaching processes. However, gaps remain in understanding the microbial community structure and functional interactions between key species and critical metabolic genes. This study addressed these questions through iron-reducing microbe-mediated bioleaching experiments combined with multi-omic approaches. The study found four major findings: (1) Bioleaching significantly enhanced the whiteness of kaolin from 60.8 to 90.4 % by selectively removing Fe (III), reducing the Fe (III) content from 1.5 % to 1.2 % without altering the kaolin structure. This process was accompanied by the solubilization of Fe (II). (2) Community succession identified <em>Clostridium</em> (87.7 %), <em>Lysinibacillus</em> (5.9 %), and <em>Bacillus</em> (0.7 %) as the dominant populations. Spearman analysis confirmed strong positive correlations between <em>Clostridium</em> abundance, Fe<sup>2+</sup> concentration, and whiteness (<em>p</em> < 0.05), suggesting that together with Fe (III) content in kaolin <em>Clostridium</em> plays a key role in whitening. (3) Metagenomic reconstruction revealed <em>Clostridium pasteurianum</em> (50.8 %) was the core functional species. Its complete glycolysis/pyruvate metabolism pathways and acidogenesis gene clusters (lactic/formic/butyric acids) synergistically facilitated Fe (III) dissolution. Notably, this strain exhibited enriched ferredoxin-coding genes and membrane-bound electron transport chain components, suggesting it had a pivotal role in dissimilatory iron reduction. This work provides the first elucidation of structure-function relationships and metabolic networks within iron-reducing consortia, offering both theoretical foundations and practical strategies for sustainable mineral bio-processing.</div></div>\",\"PeriodicalId\":245,\"journal\":{\"name\":\"Applied Clay Science\",\"volume\":\"278 \",\"pages\":\"Article 107998\"},\"PeriodicalIF\":5.8000,\"publicationDate\":\"2025-09-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Clay Science\",\"FirstCategoryId\":\"89\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0169131725003035\",\"RegionNum\":2,\"RegionCategory\":\"地球科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Clay Science","FirstCategoryId":"89","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0169131725003035","RegionNum":2,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
Clostridium pasteurianum in kaolin bioleaching: Iron reduction and whitening mechanisms
Previous studies have shown that microbial consortia outperform single strains in bioleaching processes. However, gaps remain in understanding the microbial community structure and functional interactions between key species and critical metabolic genes. This study addressed these questions through iron-reducing microbe-mediated bioleaching experiments combined with multi-omic approaches. The study found four major findings: (1) Bioleaching significantly enhanced the whiteness of kaolin from 60.8 to 90.4 % by selectively removing Fe (III), reducing the Fe (III) content from 1.5 % to 1.2 % without altering the kaolin structure. This process was accompanied by the solubilization of Fe (II). (2) Community succession identified Clostridium (87.7 %), Lysinibacillus (5.9 %), and Bacillus (0.7 %) as the dominant populations. Spearman analysis confirmed strong positive correlations between Clostridium abundance, Fe2+ concentration, and whiteness (p < 0.05), suggesting that together with Fe (III) content in kaolin Clostridium plays a key role in whitening. (3) Metagenomic reconstruction revealed Clostridium pasteurianum (50.8 %) was the core functional species. Its complete glycolysis/pyruvate metabolism pathways and acidogenesis gene clusters (lactic/formic/butyric acids) synergistically facilitated Fe (III) dissolution. Notably, this strain exhibited enriched ferredoxin-coding genes and membrane-bound electron transport chain components, suggesting it had a pivotal role in dissimilatory iron reduction. This work provides the first elucidation of structure-function relationships and metabolic networks within iron-reducing consortia, offering both theoretical foundations and practical strategies for sustainable mineral bio-processing.
期刊介绍:
Applied Clay Science aims to be an international journal attracting high quality scientific papers on clays and clay minerals, including research papers, reviews, and technical notes. The journal covers typical subjects of Fundamental and Applied Clay Science such as:
• Synthesis and purification
• Structural, crystallographic and mineralogical properties of clays and clay minerals
• Thermal properties of clays and clay minerals
• Physico-chemical properties including i) surface and interface properties; ii) thermodynamic properties; iii) mechanical properties
• Interaction with water, with polar and apolar molecules
• Colloidal properties and rheology
• Adsorption, Intercalation, Ionic exchange
• Genesis and deposits of clay minerals
• Geology and geochemistry of clays
• Modification of clays and clay minerals properties by thermal and physical treatments
• Modification by chemical treatments with organic and inorganic molecules(organoclays, pillared clays)
• Modification by biological microorganisms. etc...