利用超临界流体技术制备视黄醇聚乳酸-羟基乙酸纳米颗粒：基于析因设计的优化、理化表征和细胞活力评估

IF 3.9 3区工程技术 Q2 ENGINEERING, CHEMICAL

Chemical Engineering Research & Design Pub Date : 2025-09-19 DOI:10.1016/j.cherd.2025.09.033

Milind Velhal , Shriya Pande , Ritesh Fule , Nitin Dumore , Md Ali Mujtaba , Ali F. Almutairy , Ameeduzzafar Zafar , Mohammad Khalid

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引用次数: 0

摘要

视黄醇醋酸酯（RA）具有潜在的抗肿瘤和化学预防活性。然而，类风湿性关节炎是高度疏水性的，这降低了它的生物利用度，限制了它的治疗潜力。因此，本研究旨在利用超临界流体技术（SFT）制备聚乳酸-羟基乙酸（PLGA）纳米粒子（NPs）并对其进行表征。此外，通过细胞毒性试验评估了负载ra的PLGA-NPs的治疗效果。在本研究中，我们探索了一种新的绿色制备PLGA-NPs的技术，即混合和超声改性超临界溶液（RESS）的快速膨胀。在选择依赖因素和独立因素时，要考虑预优化可行性研究。23采用全因子设计量化因因素对独立因素的影响。对优化后的ra负载PLGA-NPs配方进行了参数表征。优化后的PLGA-NPs平均粒径为100.8 ± 10 nm，多分散性指数为0.412 ± 0.08，zeta电位为- 17.6 mV。药物含量为82 ± 5 %，包封率为99.6 ± 0.32 %。NPs的XRD和DSC分析表明该药物为非晶态。FTIR光谱证实了RA在PLGA-NPs中的有效掺入。SEM和TEM研究显示70-100 nm大小的形状均匀的颗粒。RA- plga纳米颗粒的药物释放受RA的初始扩散和随后可生物降解聚合物的降解调节。MTT实验显示，与游离RA相比，负载RA的PLGA-NPs在抑制细胞生长方面更优越。综上所述，SFT制备的ra包封PLGA-NPs作为一种药物传递系统在临床抗癌治疗中具有很大的应用潜力。

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Development of poly (lactic-Co-glycolic acid) nanoparticles of retinol using supercritical fluid technology: Factorial design-based optimization, physicochemical characterization and cell viability assessment

Retinol acetate (RA) has potential antineoplastic and chemo-preventive activities. However, RA is highly hydrophobic, which decreases its bioavailability and limits its therapeutic potential. Therefore, this study aimed to develop and characterize poly lactic-co-glycolic acid (PLGA) nanoparticles (NPs) using supercritical fluid technology (SFT). Additionally, the therapeutic effectiveness of RA-loaded PLGA-NPs was evaluated using cytotoxicity assay. In the present investigation, we have explored novel, green technology for preparing PLGA-NPs, which is the modified rapid expansion of supercritical solution (RESS) with mixing and sonication. Preoptimization feasibility studies were taken into consideration when choosing dependent and independent factors. 2³ full factorial design was applied to quantify the effect of dependent factors on independent factors. The optimized RA-loaded PLGA-NPs formulation was characterized by various parameters. The average particle size, polydispersity index, and zeta potential of optimized PLGA-NPs were 100.8 ± 10 nm, 0.412 ± 0.08, and −17.6 mV, respectively. The drug content was observed to be 82 ± 5 % and % entrapment efficiency was found to be 99.6 ± 0.32 %. The XRD and DSC of NPs showed that the drug is in a non-crystalline state. FTIR spectroscopy confirmed the effective incorporation of RA into the PLGA-NPs. SEM and TEM studies exhibit uniform-shaped particles of 70–100 nm size. The release of the drug from RA-PLGA nanoparticles was regulated by the initial diffusion of RA and later by the degradation of the biodegradable polymer. MTT assay revealed that the RA-loaded PLGA-NPs were superior in arresting cell growth as compared to free RA. In summary, RA-encapsulated PLGA-NPs, prepared by the SFT, have great potential as a drug delivery system for clinical applications in anti-cancer treatment.

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来源期刊

Chemical Engineering Research & Design 工程技术-工程：化工

CiteScore

6.10

自引率

7.70%

发文量

623

审稿时长

42 days

期刊介绍： ChERD aims to be the principal international journal for publication of high quality, original papers in chemical engineering. Papers showing how research results can be used in chemical engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in plant or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of traditional chemical engineering.