酪氨酸酶功能化石墨烯纳米纤维/AuNP生物传感器用于临床检测碱性磷酸酶

IF 3.4 4区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Journal of the Indian Chemical Society Pub Date : 2025-09-11 DOI:10.1016/j.jics.2025.102110

Preeti Kumari, Minakshi Sharma

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引用次数: 0

摘要

研究了一种基于石墨烯纳米纤维的电化学生物传感策略，用于酪氨酸酶的超灵敏和特异性ALP检测。利用纳米金修饰GNFs作为电化学检测的信号放大平台。利用GNFs/AuNPs/酪氨酸酶修饰的PG表面作为ALP活性测定的催化扩增平台。ALP作为广泛的临床疾病，特别是肝脏和骨骼疾病的生物标志物。所制备传感器的最佳pH、温度和磷酸苯基底物浓度分别为8℃、30℃和0.1 mM。该传感器具有良好的选择性和灵敏度，动态线性范围为1 ~ 2200U/L，检出限为1U/L，重现性较好。本文成功地分析了健康人血清和肝病患者血清中ALP的检测方法，该方法简便、快速，结果良好。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A tyrosinase-functionalized graphene Nanofiber/AuNP biosensor for clinical detection of alkaline phosphatase

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A tyrosinase-functionalized graphene Nanofiber/AuNP biosensor for clinical detection of alkaline phosphatase

A novel graphene nanofiber-based electrochemical biosensing strategy was developed for ultrasensitive and specific ALP detection using the tyrosinase enzyme. Gold nanoparticles decorated GNFs were utilized as a signal amplification platform for electrochemical detection. GNFs/AuNPs/Tyrosinase modified PG surface utilized as a catalytic amplification platform for ALP activity determination. ALP act as biomarker for wide range of clinical conditions specially, liver and bone disorders. The optimum pH, temperature, and phenyl phosphate substrate concentration for the fabricated sensor were 8, 30 °C, and 0.1 mM, respectively. The proposed biosensor exhibits excellent selectivity and sensitivity with a dynamic linear range from 1 to 2200U/L with a detection limit of 1U/L, along with better reproducibility. Human serum samples of healthy individuals and liver patients were successfully analyzed for ALP detection with good results via a simple and rapid approach.

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来源期刊

Journal of the Indian Chemical Society 化学-化学综合

CiteScore

3.50

自引率

7.70%

发文量

492

审稿时长

3-8 weeks

期刊介绍： The Journal of the Indian Chemical Society publishes original, fundamental, theorical, experimental research work of highest quality in all areas of chemistry, biochemistry, medicinal chemistry, electrochemistry, agrochemistry, chemical engineering and technology, food chemistry, environmental chemistry, etc.