使用运动敏感触发干涉法进行罕见细胞分类的无标记成像流式细胞术

IF 5.4 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
Lab on a Chip Pub Date : 2025-09-23 DOI:10.1039/d5lc00634a
Eden Dotan, Dana Yagoda-Aharoni, Eli Shapira, Natan T. Shaked
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引用次数: 0

摘要

我们提出了一种无标签成像流式细胞术系统,该系统集成了一个由运动敏感(基于事件)相机成像的微流控芯片和一个使用简单帧相机的干涉相位显微镜模块。事件相机以每秒数千帧的速度捕捉流动细胞的活动,当通过单个原始干涉图分析检测到需要更灵敏分析的稀有细胞时,触发明显较慢的干涉相机,从而大大减少了数据量。原始干涉测量数据作为稀有细胞分类的深度神经网络的输入。我们演示了使用该系统来检测和分级血液中的罕见癌细胞,其中事件相机用于快速区分普通白细胞和罕见癌细胞,干涉相机用于分级癌细胞类型(原发/转移),作为液体活检中检测和分级循环肿瘤细胞的人体模型。这种混合方法能够实现高效的数据采集、快速处理和高灵敏度,显著降低计算负荷,有望在成像流式细胞术中检测和处理稀有细胞方面找到各种应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Label-Free Imaging Flow Cytometry with Rare Cell Classification using Motion-Sensitive-Triggered Interferometry
We present a label-free imaging flow cytometry system that integrates a microfluidic chip imaged by a motion-sensitive (event-based) camera and an interferometric-phase-microscopy module using a simple frame-based camera. The event camera captures activity from the flowing cells corresponding to thousands of frames per second and triggers the significantly slower interferometric camera when a rare cell, requiring more sensitive analysis, is detected via a single raw-interferogram analysis, significantly reduicng data volume. The raw interferometric data serves as an input to a deep neural network for rare-cell classification. We demonstrate using this system to detect and grade rare cancer cells in blood, where the event camera is used to rapidly classify between the common white blood cells and the rare cancer cells, and the interferometric camera is used to grade the cancer cell type (primary/metastatic), as a human model for detecting and grading circulating tumor cells in liquid biopsies. This hybrid approach enables efficient data acquisition, rapid processing, and high sensitivity, significantly reducing computational load, and is expected to find various applications in detecting and processing rare cells in imaging flow cytometry.
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来源期刊
Lab on a Chip
Lab on a Chip 工程技术-化学综合
CiteScore
11.10
自引率
8.20%
发文量
434
审稿时长
2.6 months
期刊介绍: Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.
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