橄榄Kwoniella olivae f.a. sp. nov.，一种与橄榄生态系统相关的担子菌酵母。

IF 2 3区生物学 Q4 MICROBIOLOGY

International journal of systematic and evolutionary microbiology Pub Date : 2025-09-01 DOI:10.1099/ijsem.0.006916

Michele Avesani, Claudia Carvalho, Cleo Gertrud Conacher, Heidi Christa Steffen, Alfred Botha, Giacomo Zapparoli, Jose Paulo Sampaio

{"title":"橄榄Kwoniella olivae f.a. sp. nov.，一种与橄榄生态系统相关的担子菌酵母。","authors":"Michele Avesani, Claudia Carvalho, Cleo Gertrud Conacher, Heidi Christa Steffen, Alfred Botha, Giacomo Zapparoli, Jose Paulo Sampaio","doi":"10.1099/ijsem.0.006916","DOIUrl":null,"url":null,"abstract":"Three yeast strains of the basidiomycetous genus Kwoniella (subphylum Agaricomycotina; class Tremellomycetes; subclass Tremellomycetidae) were isolated from samples of olive oil collected in northern Italy. Analysis of the D1/D2 domains of the 26S rRNA gene (LSU) and of the complete internal transcribed spacer (ITS) region indicated that these strains displayed high sequence similarity to three other strains isolated from insect frass in olive trees in South Africa, suggesting that they are conspecific. Phylogenetic analyses based on LSU and ITS sequences placed these six strains in a well-supported clade closely related to Kwoniella newhampshirensis, Kwoniella shandongensis and Kwoniella lonicerae with sequence divergence of 1.2-1.6% (7-9 nucleotide substitutions) in the D1/D2 domains and 1.8-6.4% (12-37 nucleotide substitutions) in the ITS regions. Based on these results and on phenotypic analyses, a novel species, Kwoniella olivae f.a. sp. nov. PYCC 9971T (=CBS 18666T), is proposed. The MycoBank number is MB 860006.","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Kwoniella olivae f.a. sp. nov., a novel basidiomycetous yeast species associated with olive ecosystems.\",\"authors\":\"Michele Avesani, Claudia Carvalho, Cleo Gertrud Conacher, Heidi Christa Steffen, Alfred Botha, Giacomo Zapparoli, Jose Paulo Sampaio\",\"doi\":\"10.1099/ijsem.0.006916\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Three yeast strains of the basidiomycetous genus Kwoniella (subphylum Agaricomycotina; class Tremellomycetes; subclass Tremellomycetidae) were isolated from samples of olive oil collected in northern Italy. Analysis of the D1/D2 domains of the 26S rRNA gene (LSU) and of the complete internal transcribed spacer (ITS) region indicated that these strains displayed high sequence similarity to three other strains isolated from insect frass in olive trees in South Africa, suggesting that they are conspecific. Phylogenetic analyses based on LSU and ITS sequences placed these six strains in a well-supported clade closely related to Kwoniella newhampshirensis, Kwoniella shandongensis and Kwoniella lonicerae with sequence divergence of 1.2-1.6% (7-9 nucleotide substitutions) in the D1/D2 domains and 1.8-6.4% (12-37 nucleotide substitutions) in the ITS regions. Based on these results and on phenotypic analyses, a novel species, Kwoniella olivae f.a. sp. nov. PYCC 9971T (=CBS 18666T), is proposed. The MycoBank number is MB 860006.\",\"PeriodicalId\":14390,\"journal\":{\"name\":\"International journal of systematic and evolutionary microbiology\",\"volume\":\"75 9\",\"pages\":\"\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of systematic and evolutionary microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1099/ijsem.0.006916\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of systematic and evolutionary microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1099/ijsem.0.006916","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

从意大利北部采集的橄榄油样品中分离到3株Kwoniella担子菌属（Agaricomycotina亚门；银耳菌纲；银耳菌亚科）酵母菌。对26S rRNA基因（LSU）的D1/D2结构域和完整的内部转录间隔区（ITS）区域的分析表明，这些菌株与从南非橄榄树虫砂中分离的其他三株菌株具有高度的序列相似性，表明它们是同种的。基于LSU和ITS序列的系统发育分析表明，这6株菌株与Kwoniella newhampshirensis， Kwoniella山东kwoniensis和Kwoniella lonicerae在D1/D2区域的序列差异为1.2-1.6%（7-9个核苷酸取代），ITS区域的序列差异为1.8-6.4%（12-37个核苷酸取代），与Kwoniella newhamshirensis密切相关。基于这些结果和表型分析，我们提出了一个新种——Kwoniella olivae f.a. sp. nov. PYCC 9971T （=CBS 18666T）。分枝库编号为mb860006。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Kwoniella olivae f.a. sp. nov., a novel basidiomycetous yeast species associated with olive ecosystems.

Three yeast strains of the basidiomycetous genus Kwoniella (subphylum Agaricomycotina; class Tremellomycetes; subclass Tremellomycetidae) were isolated from samples of olive oil collected in northern Italy. Analysis of the D1/D2 domains of the 26S rRNA gene (LSU) and of the complete internal transcribed spacer (ITS) region indicated that these strains displayed high sequence similarity to three other strains isolated from insect frass in olive trees in South Africa, suggesting that they are conspecific. Phylogenetic analyses based on LSU and ITS sequences placed these six strains in a well-supported clade closely related to Kwoniella newhampshirensis, Kwoniella shandongensis and Kwoniella lonicerae with sequence divergence of 1.2-1.6% (7-9 nucleotide substitutions) in the D1/D2 domains and 1.8-6.4% (12-37 nucleotide substitutions) in the ITS regions. Based on these results and on phenotypic analyses, a novel species, Kwoniella olivae f.a. sp. nov. PYCC 9971^T (=CBS 18666^T), is proposed. The MycoBank number is MB 860006.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.