耐甲氧西林波希姆大coccoides bohemicum菌株新型多耐药质粒上恶唑烷酮耐药基因cfr和optrA的共存。

IF 3.6 2区 医学 Q1 INFECTIOUS DISEASES
Yao Zhu, Susu Du, Stefan Schwarz, Jie Hou, Qiu Xu, Longhua Lin, Jiyun Chai, Caiping Ma, Hongfei Sun, Shuangyu Xie, Yongshan Song, Wanjiang Zhang
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引用次数: 0

摘要

目的:鉴定鸡源耐甲氧西林波西米亚大球菌耐恶唑烷酮基因cfr和optrA。方法:采用聚合酶链反应(PCR)法检测流动恶唑烷酮耐药基因的存在。采用微量肉汤稀释法进行药敏试验。进行转移实验以评估质粒的水平转移能力。WGS采用Illumina NovaSeq/Oxford Nanopore PromethION平台组合进行。结果:波希米亚分枝杆菌HLJ23株呈现MDR表型,cfr和optrA基因均阳性。WGS结果显示,cfr和optrA基因在新型MDR质粒pHLJ23-71kb上共存。虽然偶联实验没有成功,但质粒pHLJ23-71kb可以通过电转化转移到金黄色葡萄球菌RN4220上。遗传环境分析表明,cfr和optrA与另外4个耐药基因位于质粒pHLJ23-71kb的MDR区。序列分析提示该耐多药区可能来源于哺乳球菌或葡萄球菌。结论:本研究首次在大球菌属中发现恶唑烷酮耐药基因cfr和optrA。此外,应注意葡萄球菌、哺乳球菌和大球菌属成员之间耐药性决定因素的交换。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Co-existence of the oxazolidinone resistance genes cfr and optrA on a novel multiresistance plasmid from a methicillin-resistant Macrococcoides bohemicum strain.

Objectives: To identify and characterize the oxazolidinone resistance genes cfr and optrA from a methicillin-resistant Macrococcoides bohemicum strain of chicken origin.

Methods: The presence of mobile oxazolidinone resistance genes was detected by PCR. Antimicrobial susceptibility testing was conducted by broth microdilution. Transfer experiments were carried out to evaluate horizontal transferability of the plasmid. WGS was performed using a combination of Illumina NovaSeq/Oxford Nanopore PromethION platforms.

Results: The M. bohemicum strain HLJ23 exhibited an MDR phenotype and was positive for both cfr and optrA genes. WGS revealed that the genes cfr and optrA co-exist on the novel MDR plasmid pHLJ23-71kb. Although conjugation experiments were unsuccessful, plasmid pHLJ23-71kb could be transferred to Staphylococcus aureus RN4220 by electrotransformation. Genetic context analysis showed that the cfr and optrA together with another four antimicrobial resistance genes are located in an MDR region on plasmid pHLJ23-71kb. Sequence analysis suggested that this MDR region possibly originated from Mammaliicoccus or Staphylococcus spp.

Conclusions: To the best of our knowledge, this study represents the first report of the oxazolidinone resistance genes cfr and optrA in the genus Macrococcoides. Furthermore, attention should be paid to the exchange of resistance determinants between members of the genera Staphylococcus, Mammaliicoccus and Macrococcoides.

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来源期刊
CiteScore
9.20
自引率
5.80%
发文量
423
审稿时长
2-4 weeks
期刊介绍: The Journal publishes articles that further knowledge and advance the science and application of antimicrobial chemotherapy with antibiotics and antifungal, antiviral and antiprotozoal agents. The Journal publishes primarily in human medicine, and articles in veterinary medicine likely to have an impact on global health.
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