{"title":"基于有机硅烷功能化纤维素微纤化低温支架的细胞外基质三维细胞培养","authors":"Lara Vasconcellos Ponsoni, Marina Kauling de Almeida, Beatriz Tomé, Natasha Maurmann, Anna Luíza Kern, Patricia Pranke, Sabrina Arcaro, Beatriz Merillas Valero, Matheus Vinicius Gregory Zimmermann","doi":"10.1007/s10570-025-06732-3","DOIUrl":null,"url":null,"abstract":"<div><p>Three-dimensional porous scaffolds play an important role in tissue support and growth and are widely used in cell culture within the extracellular matrix (ECM). This approach represents a significant advancement in the biomedical field, enabling the replacement of traditional two-dimensional (2D) cell cultures and reducing the need for animal testing, which presents physiological, metabolic, and high-cost limitations. Among the promising materials for scaffold development, cellulose stands out as a renewable and biocompatible biopolymer whose structural properties can be adjusted through different drying processes and chemical modifications. Despite advances in the use of cellulose aerogels and cryogels as biomaterials, the influence of different organosilanes in the functionalization of these structures remains underexplored, particularly regarding cell adhesion and proliferation. In this context, this study aimed to develop and characterize scaffolds based on microfibrillated cellulose cryogels obtained through freeze-drying and functionalized via vapor deposition with different organosilanes: tetraethoxysilane (TEOS), triethoxyvinylsilane (TEVS), 3-aminopropyltriethoxysilane (APTES), and 3-glycidyloxypropyltrimethoxysilane (GPTMS). The cryogels were characterized in terms of their morphology, chemical properties and cytotoxicity. Cryogels treated with TEOS and GPTMS demonstrated better adhesion and cell viability in assays with MRC-5 fibroblasts and PC-12 neural cells, making them promising candidates for 3D cell culture applications. The results of this study demonstrate that functionalizing cellulose cryogels enhances cell adhesion and proliferation, establishing these materials as potential scaffolds for cell culture and tissue engineering. These findings contribute to advancing the replacement of 2D models and in vivo assays, promoting the development of more effective biomimetic systems for biomedical applications.</p></div>","PeriodicalId":511,"journal":{"name":"Cellulose","volume":"32 14","pages":"8419 - 8433"},"PeriodicalIF":4.8000,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Scaffolds based on organosilane-functionalized cellulose microfibrillated cryogels for 3D cell culture in extracellular matrix\",\"authors\":\"Lara Vasconcellos Ponsoni, Marina Kauling de Almeida, Beatriz Tomé, Natasha Maurmann, Anna Luíza Kern, Patricia Pranke, Sabrina Arcaro, Beatriz Merillas Valero, Matheus Vinicius Gregory Zimmermann\",\"doi\":\"10.1007/s10570-025-06732-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Three-dimensional porous scaffolds play an important role in tissue support and growth and are widely used in cell culture within the extracellular matrix (ECM). This approach represents a significant advancement in the biomedical field, enabling the replacement of traditional two-dimensional (2D) cell cultures and reducing the need for animal testing, which presents physiological, metabolic, and high-cost limitations. Among the promising materials for scaffold development, cellulose stands out as a renewable and biocompatible biopolymer whose structural properties can be adjusted through different drying processes and chemical modifications. Despite advances in the use of cellulose aerogels and cryogels as biomaterials, the influence of different organosilanes in the functionalization of these structures remains underexplored, particularly regarding cell adhesion and proliferation. In this context, this study aimed to develop and characterize scaffolds based on microfibrillated cellulose cryogels obtained through freeze-drying and functionalized via vapor deposition with different organosilanes: tetraethoxysilane (TEOS), triethoxyvinylsilane (TEVS), 3-aminopropyltriethoxysilane (APTES), and 3-glycidyloxypropyltrimethoxysilane (GPTMS). The cryogels were characterized in terms of their morphology, chemical properties and cytotoxicity. Cryogels treated with TEOS and GPTMS demonstrated better adhesion and cell viability in assays with MRC-5 fibroblasts and PC-12 neural cells, making them promising candidates for 3D cell culture applications. The results of this study demonstrate that functionalizing cellulose cryogels enhances cell adhesion and proliferation, establishing these materials as potential scaffolds for cell culture and tissue engineering. These findings contribute to advancing the replacement of 2D models and in vivo assays, promoting the development of more effective biomimetic systems for biomedical applications.</p></div>\",\"PeriodicalId\":511,\"journal\":{\"name\":\"Cellulose\",\"volume\":\"32 14\",\"pages\":\"8419 - 8433\"},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2025-08-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cellulose\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10570-025-06732-3\",\"RegionNum\":2,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MATERIALS SCIENCE, PAPER & WOOD\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cellulose","FirstCategoryId":"88","ListUrlMain":"https://link.springer.com/article/10.1007/s10570-025-06732-3","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, PAPER & WOOD","Score":null,"Total":0}
Scaffolds based on organosilane-functionalized cellulose microfibrillated cryogels for 3D cell culture in extracellular matrix
Three-dimensional porous scaffolds play an important role in tissue support and growth and are widely used in cell culture within the extracellular matrix (ECM). This approach represents a significant advancement in the biomedical field, enabling the replacement of traditional two-dimensional (2D) cell cultures and reducing the need for animal testing, which presents physiological, metabolic, and high-cost limitations. Among the promising materials for scaffold development, cellulose stands out as a renewable and biocompatible biopolymer whose structural properties can be adjusted through different drying processes and chemical modifications. Despite advances in the use of cellulose aerogels and cryogels as biomaterials, the influence of different organosilanes in the functionalization of these structures remains underexplored, particularly regarding cell adhesion and proliferation. In this context, this study aimed to develop and characterize scaffolds based on microfibrillated cellulose cryogels obtained through freeze-drying and functionalized via vapor deposition with different organosilanes: tetraethoxysilane (TEOS), triethoxyvinylsilane (TEVS), 3-aminopropyltriethoxysilane (APTES), and 3-glycidyloxypropyltrimethoxysilane (GPTMS). The cryogels were characterized in terms of their morphology, chemical properties and cytotoxicity. Cryogels treated with TEOS and GPTMS demonstrated better adhesion and cell viability in assays with MRC-5 fibroblasts and PC-12 neural cells, making them promising candidates for 3D cell culture applications. The results of this study demonstrate that functionalizing cellulose cryogels enhances cell adhesion and proliferation, establishing these materials as potential scaffolds for cell culture and tissue engineering. These findings contribute to advancing the replacement of 2D models and in vivo assays, promoting the development of more effective biomimetic systems for biomedical applications.
期刊介绍:
Cellulose is an international journal devoted to the dissemination of research and scientific and technological progress in the field of cellulose and related naturally occurring polymers. The journal is concerned with the pure and applied science of cellulose and related materials, and also with the development of relevant new technologies. This includes the chemistry, biochemistry, physics and materials science of cellulose and its sources, including wood and other biomass resources, and their derivatives. Coverage extends to the conversion of these polymers and resources into manufactured goods, such as pulp, paper, textiles, and manufactured as well natural fibers, and to the chemistry of materials used in their processing. Cellulose publishes review articles, research papers, and technical notes.