肝炎肝硬化患者肝内HDV RNA水平的大空间变异与HBV核心或S RNA水平无关

IF 3.4 3区医学 Q2 VIROLOGY

Journal of Clinical Virology Pub Date : 2025-09-12 DOI:10.1016/j.jcv.2025.105871

Gustaf E. Rydell , Lucia Gonzales Strömberg , Johan Ringlander , Maria E. Andersson , Catarina Skoglund , Joakim Bedner Stenbäck , Staffan Nilsson , Maria Castedal , Magnus Lindh

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引用次数: 0

摘要

本研究的目的是探讨肝内HDV RNA、HBV RNA和相应血清标志物水平在因HDV诱导的肝病而接受肝移植的患者中的相关性。方法采用数字液滴PCR对5例hdv肝移植患者各10块肝组织进行分析。结果患者体内和患者之间的病毒RNA组织水平差异较大。总体而言，HBV核心和S RNA的组织水平呈正相关。然而，除了一名患者外，没有观察到HDV RNA与核心RNA或S RNA组织水平之间的一致关联。此外，肝内HDV RNA水平与血清HDV RNA无关。相反，血清HDV RNA与血清HBsAg呈正相关，与组织HBV S RNA呈相关趋势，与核心RNA水平呈显著相关。结论肝内HBsAg可能是HDV颗粒分泌的限制因素，但不支持HDV抑制HBV复制的假设。

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Large spatial variation of intrahepatic HDV RNA levels without association with HBV core or S RNA levels in HDV cirrhosis patients

Background

The aim of this study was to investigate correlations between levels of intrahepatic HDV RNA, HBV RNA and corresponding serum markers in patients who underwent transplantation because of HDV-induced liver disease.

Methods

10 pieces of tissue from each of five liver explants from patients that underwent transplantation because of HDV-induced liver disease were analyzed by digital droplet PCR.

Results

A large variation of the tissue levels of viral RNA was found both between and within patients. Overall, tissue levels of HBV core and S RNA were positively associated. However, no consistent association was observed between tissue levels of HDV RNA and either core or S RNA, except in one patient. Furthermore, intrahepatic HDV RNA levels did not correlate with serum HDV RNA. Instead, serum HDV RNA showed a positive correlation with serum HBsAg, a trend towards correlation with tissue HBV S RNA and a significant correlation with core RNA levels.

Conclusions

The results suggest that intrahepatic HBsAg might be a limiting factor for HDV particle secretion, but do not support the hypothesis that HDV suppresses HBV replication.

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来源期刊

Journal of Clinical Virology 医学-病毒学

CiteScore

22.70

自引率

1.10%

发文量

149

审稿时长

24 days

期刊介绍： The Journal of Clinical Virology, an esteemed international publication, serves as the official journal for both the Pan American Society for Clinical Virology and The European Society for Clinical Virology. Dedicated to advancing the understanding of human virology in clinical settings, the Journal of Clinical Virology focuses on disseminating research papers and reviews pertaining to the clinical aspects of virology. Its scope encompasses articles discussing diagnostic methodologies and virus-induced clinical conditions, with an emphasis on practicality and relevance to clinical practice. The journal publishes on topics that include: • new diagnostic technologies • nucleic acid amplification and serologic testing • targeted and metagenomic next-generation sequencing • emerging pandemic viral threats • respiratory viruses • transplant viruses • chronic viral infections • cancer-associated viruses • gastrointestinal viruses • central nervous system viruses • one health (excludes animal health)