F.H. Meng , L. Wang , T. Zhou , L.W. Chang , Y.Y. Zi , R.N. Su , M. Zhao , X.Y. Zhou , D. Zhang , W.G. Zhang , C.X. Liu
{"title":"苏尼特蒙古羊骨骼肌血管扩张途径在适应性产热中的作用","authors":"F.H. Meng , L. Wang , T. Zhou , L.W. Chang , Y.Y. Zi , R.N. Su , M. Zhao , X.Y. Zhou , D. Zhang , W.G. Zhang , C.X. Liu","doi":"10.1016/j.jtherbio.2025.104278","DOIUrl":null,"url":null,"abstract":"<div><div>The Sunite Mongolian sheep is one of the superior livestock breeds in Inner Mongolia, capable of surviving and reproducing on the Xilingol Grassland at temperatures below −30 °C, demonstrating remarkable cold adaptation. However, research on adaptive thermogenesis in its muscle tissue remains limited. To investigate skeletal muscle's role in adaptive thermogenesis, shoulder muscle tissues were collected from three healthy castrated male sheep each in winter (January) and summer (July) for RNA sequencing (RNA-seq) and differential expression analysis. Differentially expressed genes (DEGs) were primarily enriched in pathways associated with starvation response, hormonal regulation, and energy metabolism. Gene expression analysis revealed significant upregulation of nitric oxide synthase 3 (<em>NOS3</em>) and adrenergic receptor-β2 (ADRB2) in the adrenergic-cyclic guanosine monophosphate (cGMP)- protein kinase G(PKG)vasodilatory signaling pathway during winter (<em>P</em> < 0.01). Further validation showed that ADRB2 was extensively expressed in skeletal muscle vascular endothelial cells, with significantly increased winter expression. Subsequent in vivo experiments confirmed these results, intravenous injection of isoproterenol (ISO) dose-dependently increased cGMP concentration and <em>NOS3</em> expression (<em>P</em> < 0.05). In summary, we speculate that epinephrine binds to ADRB2 in the skeletal muscle of Sunite Mongolian sheep during winter, activating intracellular <em>NOS3</em> to synthesize nitric oxide (NO). Through the cGMP-PKG signaling pathway, this induces vasodilation, thereby transporting more nutrients and enhancing metabolic energy production and heat generation, primarily via glucose metabolism, to adapt to cold environments. This study provides new insights into cold adaptation in sheep, suggesting ADRB2/NOS3 as potential molecular markers for breeding cold-resistant breeds to enhance farming efficiency.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"133 ","pages":"Article 104278"},"PeriodicalIF":2.9000,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Role of skeletal muscle vasodilatory pathway in adaptive thermogenesis in Sunit Mongolian sheep\",\"authors\":\"F.H. Meng , L. Wang , T. Zhou , L.W. Chang , Y.Y. Zi , R.N. Su , M. Zhao , X.Y. Zhou , D. Zhang , W.G. Zhang , C.X. Liu\",\"doi\":\"10.1016/j.jtherbio.2025.104278\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The Sunite Mongolian sheep is one of the superior livestock breeds in Inner Mongolia, capable of surviving and reproducing on the Xilingol Grassland at temperatures below −30 °C, demonstrating remarkable cold adaptation. However, research on adaptive thermogenesis in its muscle tissue remains limited. To investigate skeletal muscle's role in adaptive thermogenesis, shoulder muscle tissues were collected from three healthy castrated male sheep each in winter (January) and summer (July) for RNA sequencing (RNA-seq) and differential expression analysis. Differentially expressed genes (DEGs) were primarily enriched in pathways associated with starvation response, hormonal regulation, and energy metabolism. Gene expression analysis revealed significant upregulation of nitric oxide synthase 3 (<em>NOS3</em>) and adrenergic receptor-β2 (ADRB2) in the adrenergic-cyclic guanosine monophosphate (cGMP)- protein kinase G(PKG)vasodilatory signaling pathway during winter (<em>P</em> < 0.01). Further validation showed that ADRB2 was extensively expressed in skeletal muscle vascular endothelial cells, with significantly increased winter expression. Subsequent in vivo experiments confirmed these results, intravenous injection of isoproterenol (ISO) dose-dependently increased cGMP concentration and <em>NOS3</em> expression (<em>P</em> < 0.05). In summary, we speculate that epinephrine binds to ADRB2 in the skeletal muscle of Sunite Mongolian sheep during winter, activating intracellular <em>NOS3</em> to synthesize nitric oxide (NO). Through the cGMP-PKG signaling pathway, this induces vasodilation, thereby transporting more nutrients and enhancing metabolic energy production and heat generation, primarily via glucose metabolism, to adapt to cold environments. This study provides new insights into cold adaptation in sheep, suggesting ADRB2/NOS3 as potential molecular markers for breeding cold-resistant breeds to enhance farming efficiency.</div></div>\",\"PeriodicalId\":17428,\"journal\":{\"name\":\"Journal of thermal biology\",\"volume\":\"133 \",\"pages\":\"Article 104278\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-09-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of thermal biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0306456525002359\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of thermal biology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0306456525002359","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOLOGY","Score":null,"Total":0}
Role of skeletal muscle vasodilatory pathway in adaptive thermogenesis in Sunit Mongolian sheep
The Sunite Mongolian sheep is one of the superior livestock breeds in Inner Mongolia, capable of surviving and reproducing on the Xilingol Grassland at temperatures below −30 °C, demonstrating remarkable cold adaptation. However, research on adaptive thermogenesis in its muscle tissue remains limited. To investigate skeletal muscle's role in adaptive thermogenesis, shoulder muscle tissues were collected from three healthy castrated male sheep each in winter (January) and summer (July) for RNA sequencing (RNA-seq) and differential expression analysis. Differentially expressed genes (DEGs) were primarily enriched in pathways associated with starvation response, hormonal regulation, and energy metabolism. Gene expression analysis revealed significant upregulation of nitric oxide synthase 3 (NOS3) and adrenergic receptor-β2 (ADRB2) in the adrenergic-cyclic guanosine monophosphate (cGMP)- protein kinase G(PKG)vasodilatory signaling pathway during winter (P < 0.01). Further validation showed that ADRB2 was extensively expressed in skeletal muscle vascular endothelial cells, with significantly increased winter expression. Subsequent in vivo experiments confirmed these results, intravenous injection of isoproterenol (ISO) dose-dependently increased cGMP concentration and NOS3 expression (P < 0.05). In summary, we speculate that epinephrine binds to ADRB2 in the skeletal muscle of Sunite Mongolian sheep during winter, activating intracellular NOS3 to synthesize nitric oxide (NO). Through the cGMP-PKG signaling pathway, this induces vasodilation, thereby transporting more nutrients and enhancing metabolic energy production and heat generation, primarily via glucose metabolism, to adapt to cold environments. This study provides new insights into cold adaptation in sheep, suggesting ADRB2/NOS3 as potential molecular markers for breeding cold-resistant breeds to enhance farming efficiency.
期刊介绍:
The Journal of Thermal Biology publishes articles that advance our knowledge on the ways and mechanisms through which temperature affects man and animals. This includes studies of their responses to these effects and on the ecological consequences. Directly relevant to this theme are:
• The mechanisms of thermal limitation, heat and cold injury, and the resistance of organisms to extremes of temperature
• The mechanisms involved in acclimation, acclimatization and evolutionary adaptation to temperature
• Mechanisms underlying the patterns of hibernation, torpor, dormancy, aestivation and diapause
• Effects of temperature on reproduction and development, growth, ageing and life-span
• Studies on modelling heat transfer between organisms and their environment
• The contributions of temperature to effects of climate change on animal species and man
• Studies of conservation biology and physiology related to temperature
• Behavioural and physiological regulation of body temperature including its pathophysiology and fever
• Medical applications of hypo- and hyperthermia
Article types:
• Original articles
• Review articles