Joan Martí-Carreras , Johan Espunyes , Laura Carrera-Faja , Carlotta Pasetto , Maria Magdalena Alcover Amengual , Sarah Chavez-Fisa , Marina Carrasco-Martin , Xavier Roura , Olga Francino , Lluís Ferrer
{"title":"西班牙马略卡岛壁虎的小利什曼原虫和小利什曼原虫","authors":"Joan Martí-Carreras , Johan Espunyes , Laura Carrera-Faja , Carlotta Pasetto , Maria Magdalena Alcover Amengual , Sarah Chavez-Fisa , Marina Carrasco-Martin , Xavier Roura , Olga Francino , Lluís Ferrer","doi":"10.1016/j.ijppaw.2025.101138","DOIUrl":null,"url":null,"abstract":"<div><div><em>Leishmania tarentolae</em> and <em>Leishmania infantum</em> are two sympatric parasites of significant ecological and epidemiological interest in the Mediterranean basin. This study investigated the PCR prevalence of <em>L. tarentolae</em> and <em>L. infantum</em> in two gecko species (<em>Tarentola mauritanica</em> and <em>Hemidactylus turcicus</em>) present on Mallorca Island, Spain, using duplex quantitative PCR. A total of 59 geckos were sampled across the island, including 53 <em>T. mauritanica</em> and six <em>H. turcicus</em>. Tissue and blood samples were screened by PCR for both parasites. The results revealed the prevalence of <em>Leishmania</em> infection in adult <em>T. mauritanica</em>, with 10/49 (20.41 %) testing PCR positive for <em>L. tarentolae</em> only and with 1/49 (2.04 %) for <em>L. infantum</em> only. Coinfection with both parasites was detected in 3/49 geckos (6.12 %). No positives were identified in <em>H. turcicus</em>, probably due to small sample size. Regarding PCR positivity by tissues, coleomic organs were more likely to be positive for <em>L. tarentolae</em> in adult <em>T. mauritanica</em> than blood, with a slighter PCR positivity in the liver, spleen and lung. This study provides further insight into the interaction between <em>Leishmania</em> and geckos in leishmaniosis-endemic areas such as Mallorca.</div></div>","PeriodicalId":54278,"journal":{"name":"International Journal for Parasitology-Parasites and Wildlife","volume":"28 ","pages":"Article 101138"},"PeriodicalIF":2.2000,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Leishmania tarentolae and Leishmania infantum in geckos from Mallorca Island, Spain\",\"authors\":\"Joan Martí-Carreras , Johan Espunyes , Laura Carrera-Faja , Carlotta Pasetto , Maria Magdalena Alcover Amengual , Sarah Chavez-Fisa , Marina Carrasco-Martin , Xavier Roura , Olga Francino , Lluís Ferrer\",\"doi\":\"10.1016/j.ijppaw.2025.101138\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>Leishmania tarentolae</em> and <em>Leishmania infantum</em> are two sympatric parasites of significant ecological and epidemiological interest in the Mediterranean basin. This study investigated the PCR prevalence of <em>L. tarentolae</em> and <em>L. infantum</em> in two gecko species (<em>Tarentola mauritanica</em> and <em>Hemidactylus turcicus</em>) present on Mallorca Island, Spain, using duplex quantitative PCR. A total of 59 geckos were sampled across the island, including 53 <em>T. mauritanica</em> and six <em>H. turcicus</em>. Tissue and blood samples were screened by PCR for both parasites. The results revealed the prevalence of <em>Leishmania</em> infection in adult <em>T. mauritanica</em>, with 10/49 (20.41 %) testing PCR positive for <em>L. tarentolae</em> only and with 1/49 (2.04 %) for <em>L. infantum</em> only. Coinfection with both parasites was detected in 3/49 geckos (6.12 %). No positives were identified in <em>H. turcicus</em>, probably due to small sample size. Regarding PCR positivity by tissues, coleomic organs were more likely to be positive for <em>L. tarentolae</em> in adult <em>T. mauritanica</em> than blood, with a slighter PCR positivity in the liver, spleen and lung. This study provides further insight into the interaction between <em>Leishmania</em> and geckos in leishmaniosis-endemic areas such as Mallorca.</div></div>\",\"PeriodicalId\":54278,\"journal\":{\"name\":\"International Journal for Parasitology-Parasites and Wildlife\",\"volume\":\"28 \",\"pages\":\"Article 101138\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-09-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal for Parasitology-Parasites and Wildlife\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2213224425001038\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal for Parasitology-Parasites and Wildlife","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213224425001038","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ECOLOGY","Score":null,"Total":0}
Leishmania tarentolae and Leishmania infantum in geckos from Mallorca Island, Spain
Leishmania tarentolae and Leishmania infantum are two sympatric parasites of significant ecological and epidemiological interest in the Mediterranean basin. This study investigated the PCR prevalence of L. tarentolae and L. infantum in two gecko species (Tarentola mauritanica and Hemidactylus turcicus) present on Mallorca Island, Spain, using duplex quantitative PCR. A total of 59 geckos were sampled across the island, including 53 T. mauritanica and six H. turcicus. Tissue and blood samples were screened by PCR for both parasites. The results revealed the prevalence of Leishmania infection in adult T. mauritanica, with 10/49 (20.41 %) testing PCR positive for L. tarentolae only and with 1/49 (2.04 %) for L. infantum only. Coinfection with both parasites was detected in 3/49 geckos (6.12 %). No positives were identified in H. turcicus, probably due to small sample size. Regarding PCR positivity by tissues, coleomic organs were more likely to be positive for L. tarentolae in adult T. mauritanica than blood, with a slighter PCR positivity in the liver, spleen and lung. This study provides further insight into the interaction between Leishmania and geckos in leishmaniosis-endemic areas such as Mallorca.
期刊介绍:
The International Journal for Parasitology: Parasites and Wildlife (IJP-PAW) publishes the results of original research on parasites of all wildlife, invertebrate and vertebrate. This includes free-ranging, wild populations, as well as captive wildlife, semi-domesticated species (e.g. reindeer) and farmed populations of recently domesticated or wild-captured species (e.g. cultured fishes). Articles on all aspects of wildlife parasitology are welcomed including taxonomy, biodiversity and distribution, ecology and epidemiology, population biology and host-parasite relationships. The impact of parasites on the health and conservation of wildlife is seen as an important area covered by the journal especially the potential role of environmental factors, for example climate. Also important to the journal is ''one health'' and the nature of interactions between wildlife, people and domestic animals, including disease emergence and zoonoses.