Zeping Zhang , Rizheng Han , Caihao Huang , Yueen Liu , Guixue Wang , Yun Bai , Rui Yang , Tao Jin , Xing Zhang
{"title":"各向异性静电纺丝聚(ε-己内酯)/聚碳酸酯聚氨酯支架在组织工程心脏瓣膜中的抗疲劳性能","authors":"Zeping Zhang , Rizheng Han , Caihao Huang , Yueen Liu , Guixue Wang , Yun Bai , Rui Yang , Tao Jin , Xing Zhang","doi":"10.1016/j.matdes.2025.114762","DOIUrl":null,"url":null,"abstract":"<div><div>In this study, poly(ε-caprolactone) (PCL) and polycarbonate urethane (PCU) were used to fabricate electrospun scaffolds for tissue-engineered heart valves (TEHVs). The PCL/PCU scaffold containing 25 % PCU (named as 75%PCL-O) with oriented fibers exhibited pronounced anisotropy, with elastic moduli of 53.47 ± 0.93 MPa (X-axis) and 4.19 ± 0.70 MPa (Y-axis), and tensile strength of 14.21 ± 1.16 MPa (X-axis) and 1.59 ± 0.09 MPa (Y-axis), respectively, close to native heart valves. The 75%PCL-O scaffold showed good cell viability and guided cell alignment along the fibers, and no obvious hemolysis or thrombus formation. Hydrodynamic tests showed an effective orifice area (<em>EOA</em>) of 2.42 ± 0.12 cm<sup>2</sup> and a regurgitant fraction (<em>RF</em>) of 5.98 ± 2.31 % for a 25 mm surgical pulmonary valve, meeting the ISO 5840-2 standard. The accelerated fatigue testing demonstrated that the <em>EOA</em> and <em>RF</em> remained stable throughout 50 million cycles. Additionally, finite element analysis (FEA) revealed that mechanical stress concentrated at the free edge for the 75%PCL-O valve leaflet during the opening-closing cycles, correlating well with the observed fiber degradation in these regions during fatigue tests. In summary, the 75%PCL-O scaffold exhibits favorable mechanical performance, good biocompatibility and improved durability, showing great potential for TEHV applications.</div></div>","PeriodicalId":383,"journal":{"name":"Materials & Design","volume":"259 ","pages":"Article 114762"},"PeriodicalIF":7.9000,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Anisotropic electrospun poly(ε-caprolactone)/polycarbonate urethane scaffolds with improved fatigue performance for tissue-engineered heart valves\",\"authors\":\"Zeping Zhang , Rizheng Han , Caihao Huang , Yueen Liu , Guixue Wang , Yun Bai , Rui Yang , Tao Jin , Xing Zhang\",\"doi\":\"10.1016/j.matdes.2025.114762\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>In this study, poly(ε-caprolactone) (PCL) and polycarbonate urethane (PCU) were used to fabricate electrospun scaffolds for tissue-engineered heart valves (TEHVs). The PCL/PCU scaffold containing 25 % PCU (named as 75%PCL-O) with oriented fibers exhibited pronounced anisotropy, with elastic moduli of 53.47 ± 0.93 MPa (X-axis) and 4.19 ± 0.70 MPa (Y-axis), and tensile strength of 14.21 ± 1.16 MPa (X-axis) and 1.59 ± 0.09 MPa (Y-axis), respectively, close to native heart valves. The 75%PCL-O scaffold showed good cell viability and guided cell alignment along the fibers, and no obvious hemolysis or thrombus formation. Hydrodynamic tests showed an effective orifice area (<em>EOA</em>) of 2.42 ± 0.12 cm<sup>2</sup> and a regurgitant fraction (<em>RF</em>) of 5.98 ± 2.31 % for a 25 mm surgical pulmonary valve, meeting the ISO 5840-2 standard. The accelerated fatigue testing demonstrated that the <em>EOA</em> and <em>RF</em> remained stable throughout 50 million cycles. Additionally, finite element analysis (FEA) revealed that mechanical stress concentrated at the free edge for the 75%PCL-O valve leaflet during the opening-closing cycles, correlating well with the observed fiber degradation in these regions during fatigue tests. In summary, the 75%PCL-O scaffold exhibits favorable mechanical performance, good biocompatibility and improved durability, showing great potential for TEHV applications.</div></div>\",\"PeriodicalId\":383,\"journal\":{\"name\":\"Materials & Design\",\"volume\":\"259 \",\"pages\":\"Article 114762\"},\"PeriodicalIF\":7.9000,\"publicationDate\":\"2025-09-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Materials & Design\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0264127525011827\",\"RegionNum\":2,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MATERIALS SCIENCE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Materials & Design","FirstCategoryId":"88","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0264127525011827","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
Anisotropic electrospun poly(ε-caprolactone)/polycarbonate urethane scaffolds with improved fatigue performance for tissue-engineered heart valves
In this study, poly(ε-caprolactone) (PCL) and polycarbonate urethane (PCU) were used to fabricate electrospun scaffolds for tissue-engineered heart valves (TEHVs). The PCL/PCU scaffold containing 25 % PCU (named as 75%PCL-O) with oriented fibers exhibited pronounced anisotropy, with elastic moduli of 53.47 ± 0.93 MPa (X-axis) and 4.19 ± 0.70 MPa (Y-axis), and tensile strength of 14.21 ± 1.16 MPa (X-axis) and 1.59 ± 0.09 MPa (Y-axis), respectively, close to native heart valves. The 75%PCL-O scaffold showed good cell viability and guided cell alignment along the fibers, and no obvious hemolysis or thrombus formation. Hydrodynamic tests showed an effective orifice area (EOA) of 2.42 ± 0.12 cm2 and a regurgitant fraction (RF) of 5.98 ± 2.31 % for a 25 mm surgical pulmonary valve, meeting the ISO 5840-2 standard. The accelerated fatigue testing demonstrated that the EOA and RF remained stable throughout 50 million cycles. Additionally, finite element analysis (FEA) revealed that mechanical stress concentrated at the free edge for the 75%PCL-O valve leaflet during the opening-closing cycles, correlating well with the observed fiber degradation in these regions during fatigue tests. In summary, the 75%PCL-O scaffold exhibits favorable mechanical performance, good biocompatibility and improved durability, showing great potential for TEHV applications.
期刊介绍:
Materials and Design is a multi-disciplinary journal that publishes original research reports, review articles, and express communications. The journal focuses on studying the structure and properties of inorganic and organic materials, advancements in synthesis, processing, characterization, and testing, the design of materials and engineering systems, and their applications in technology. It aims to bring together various aspects of materials science, engineering, physics, and chemistry.
The journal explores themes ranging from materials to design and aims to reveal the connections between natural and artificial materials, as well as experiment and modeling. Manuscripts submitted to Materials and Design should contain elements of discovery and surprise, as they often contribute new insights into the architecture and function of matter.