用胶体金横向流动条快速检测塞尼卡病毒A

IF 2.2 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2025-09-16 DOI:10.1016/j.bbrep.2025.102240

Xiaomeng Pei , Jing Wang , Zhijing Wei , Yuanying Li , Meng Wu

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引用次数: 0

摘要

塞内卡病毒A （SVA）可引起猪的水疱性病变和其他症状，这给养猪业造成了巨大的经济损失。为了建立快速检测SVA的方法，构建了免疫层析横向流动条带。首先，纯化重组SVA VP2蛋白，制备单克隆抗体。将4B10涂在试纸线上（T线），1E3贴胶体金标签，组装胶体金试纸条。构建的LFA条带检出限为0.5 ng/mL，与FMDV、PRRSV、CSFV和PCV2无交叉反应。临床样品验证结果与RT-PCR结果的一致性达90%，表明了该方法的可靠性。所建立的侧流法（LFA）为SVA的即时检测提供了一种快速、简便的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Rapid detection of Senecavirus A using a colloidal gold lateral flow strip

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Rapid detection of Senecavirus A using a colloidal gold lateral flow strip

Senecavirus A (SVA) could cause vesicular lesions and other symptoms in pigs, which has resulted in substantial economic losses to the swine industry. To establish a rapid detection method for SVA, an immunochromatographic lateral flow strip was constructed. First, the recombinant SVA VP2 protein was purified, and the monoclonal antibodies were prepared. 4B10 was coated onto the test line (T line), and 1E3 was colloidal gold-labeled to assemble the colloidal gold test strip. The constructed LFA strip showed a detection limit of 0.5 ng/mL, and no cross-reactivity with FMDV, PRRSV, CSFV and PCV2. Clinical samples validation showed 90 % concordance with RT-PCR results, indicated the reliability of this method. The developed lateral flow assay (LFA) provided a rapid and simple method for point-of-care testing of SVA.

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.