lin28介导的基因调控环在器官发生过程中同步过渡

IF 2.2 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Indhujah Thevarajan , Maria F. Osuna , Sonia Fuentes Lewey , Eustolia Sauceda , Sayra Briseno , Caylah Griffin , Bareun Kim , R. Grant Rowe , Edroaldo Lummertz da Rocha , Jihan K. Osborne
{"title":"lin28介导的基因调控环在器官发生过程中同步过渡","authors":"Indhujah Thevarajan ,&nbsp;Maria F. Osuna ,&nbsp;Sonia Fuentes Lewey ,&nbsp;Eustolia Sauceda ,&nbsp;Sayra Briseno ,&nbsp;Caylah Griffin ,&nbsp;Bareun Kim ,&nbsp;R. Grant Rowe ,&nbsp;Edroaldo Lummertz da Rocha ,&nbsp;Jihan K. Osborne","doi":"10.1016/j.bbrep.2025.102226","DOIUrl":null,"url":null,"abstract":"<div><div>Control of the intervals between proliferation and differentiation of stem/progenitor cells is coordinated by developmental regulators, comprised of both microRNAs (miRNAs) and proteins, termed heterochronic genes. The heterochronic factors, Lin28-RNA-binding proteins (RBPs) and the miRNAs–<em>Let-7,</em> comprise a unique subset of evolutionarily conserved genes that regulate the developmental timing of metazoans, from worms to mammals. While there has been much investigation into the reciprocal negative feedback loop between LIN-28 and <em>Let-7</em> during fetal development and cancer. Few have investigated how positive regulatory loops between the mammalian Lin28-RBPs, and mRNAs order spatiotemporal transitions of progenitors from specification to organogenesis. Screening for factors that activated luciferase reporters of the human <em>LIN28A</em> and <em>LIN28B</em> promoters, in combination with genetic mouse models, we demonstrate positive feedforward loops between key developmental transcription factors such as B-Catenin, Sox2, Sox9, and Lin28-RBPs. Furthermore, we demonstrate heterochronic regulation of morphogenesis is not only genetically modulated but also molecularly fine-tuned via position-dependent sequences in the 5′ and/or 3’ untranslated regions.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102226"},"PeriodicalIF":2.2000,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"LIN28-mediated gene regulatory loops synchronize transitions throughout organogenesis\",\"authors\":\"Indhujah Thevarajan ,&nbsp;Maria F. Osuna ,&nbsp;Sonia Fuentes Lewey ,&nbsp;Eustolia Sauceda ,&nbsp;Sayra Briseno ,&nbsp;Caylah Griffin ,&nbsp;Bareun Kim ,&nbsp;R. Grant Rowe ,&nbsp;Edroaldo Lummertz da Rocha ,&nbsp;Jihan K. Osborne\",\"doi\":\"10.1016/j.bbrep.2025.102226\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Control of the intervals between proliferation and differentiation of stem/progenitor cells is coordinated by developmental regulators, comprised of both microRNAs (miRNAs) and proteins, termed heterochronic genes. The heterochronic factors, Lin28-RNA-binding proteins (RBPs) and the miRNAs–<em>Let-7,</em> comprise a unique subset of evolutionarily conserved genes that regulate the developmental timing of metazoans, from worms to mammals. While there has been much investigation into the reciprocal negative feedback loop between LIN-28 and <em>Let-7</em> during fetal development and cancer. Few have investigated how positive regulatory loops between the mammalian Lin28-RBPs, and mRNAs order spatiotemporal transitions of progenitors from specification to organogenesis. Screening for factors that activated luciferase reporters of the human <em>LIN28A</em> and <em>LIN28B</em> promoters, in combination with genetic mouse models, we demonstrate positive feedforward loops between key developmental transcription factors such as B-Catenin, Sox2, Sox9, and Lin28-RBPs. Furthermore, we demonstrate heterochronic regulation of morphogenesis is not only genetically modulated but also molecularly fine-tuned via position-dependent sequences in the 5′ and/or 3’ untranslated regions.</div></div>\",\"PeriodicalId\":8771,\"journal\":{\"name\":\"Biochemistry and Biophysics Reports\",\"volume\":\"44 \",\"pages\":\"Article 102226\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-09-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemistry and Biophysics Reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2405580825003139\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry and Biophysics Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405580825003139","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

干细胞/祖细胞增殖和分化之间的间隔由发育调节因子协调控制,发育调节因子由microrna (mirna)和蛋白质组成,称为异时基因。异慢性因子,即lin28 - rna结合蛋白(rbp)和mirna - let -7,组成了一个独特的进化保守基因子集,调节从蠕虫到哺乳动物的后生动物的发育时间。然而,在胎儿发育和癌症过程中,LIN-28和Let-7之间的反向负反馈回路已经得到了很多研究。很少有人研究哺乳动物lin28 - rbp和mrna之间的正调控回路如何命令祖细胞从规范到器官发生的时空转变。通过筛选激活人类LIN28A和LIN28B启动子荧光素酶报告基因的因子,结合小鼠遗传模型,我们证实了关键发育转录因子(如B-Catenin、Sox2、Sox9和lin28 - rbp)之间的正前馈循环。此外,我们证明了形态发生的异时调控不仅是遗传调控的,而且是通过5 ‘和/或3 ’非翻译区域的位置依赖序列进行分子微调的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
LIN28-mediated gene regulatory loops synchronize transitions throughout organogenesis
Control of the intervals between proliferation and differentiation of stem/progenitor cells is coordinated by developmental regulators, comprised of both microRNAs (miRNAs) and proteins, termed heterochronic genes. The heterochronic factors, Lin28-RNA-binding proteins (RBPs) and the miRNAs–Let-7, comprise a unique subset of evolutionarily conserved genes that regulate the developmental timing of metazoans, from worms to mammals. While there has been much investigation into the reciprocal negative feedback loop between LIN-28 and Let-7 during fetal development and cancer. Few have investigated how positive regulatory loops between the mammalian Lin28-RBPs, and mRNAs order spatiotemporal transitions of progenitors from specification to organogenesis. Screening for factors that activated luciferase reporters of the human LIN28A and LIN28B promoters, in combination with genetic mouse models, we demonstrate positive feedforward loops between key developmental transcription factors such as B-Catenin, Sox2, Sox9, and Lin28-RBPs. Furthermore, we demonstrate heterochronic regulation of morphogenesis is not only genetically modulated but also molecularly fine-tuned via position-dependent sequences in the 5′ and/or 3’ untranslated regions.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Biochemistry and Biophysics Reports
Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
4.60
自引率
0.00%
发文量
191
审稿时长
59 days
期刊介绍: Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信