Afsaneh Mousa Pour , Zahra Garkani-Nejad , Hadi Mahmoudi-Moghaddam
{"title":"基于功能化MoS 2 /聚吡咯纳米复合材料的高效dna生物传感器的开发用于丝裂霉素C的测定。","authors":"Afsaneh Mousa Pour , Zahra Garkani-Nejad , Hadi Mahmoudi-Moghaddam","doi":"10.1016/j.bioelechem.2025.109111","DOIUrl":null,"url":null,"abstract":"<div><div>In this study, plate-like Eu<sup>3+</sup>-doped MoS₂ nanoparticles (PL-Eu<sup>3+</sup>-doped MoS₂ NPs) were successfully synthesized via a simple hydrothermal method and characterized using energy-dispersive X-ray spectroscopy (EDX), field-emission scanning electron microscopy (FESEM), and X-ray diffraction (XRD). Subsequently, a novel electrochemical DNA biosensor was developed by modifying pencil graphite electrodes (PGEs) with the synthesized nanocomposite and polypyrrole (PPy) for the sensitive detection of mitomycin C (MC). The interaction between double-stranded DNA (ds-DNA) and MC was thoroughly investigated using differential pulse voltammetry (DPV), spectrophotometry, and molecular docking methods. The decrease in the oxidation peak currents of adenine base in an acetate buffer solution (pH 4.80) was employed as a reliable indicator of the interaction event. The constructed biosensor (ds-DNA/PL-Eu<sup>3+</sup>-doped MoS₂/PGE) exhibited a linear response toward MC concentrations ranging from 1 to 75 μg/mL, achieving a low detection limit of 0.8 μg/mL. The sensor's performance was further validated in real sample analyses, demonstrating excellent recovery values between 97 % and 104 %, thereby confirming its potential for practical applications in biomedical and environmental monitoring. UV/Vis spectroscopic data combined with molecular docking analyses indicated substantial interaction between mitomycin and DNA, with evidence supporting groove binding as the predominant binding mode.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109111"},"PeriodicalIF":4.5000,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development of a highly efficient DNA-based biosensor using a functionalized MoS₂/polypyrrole nanocomposite for mitomycin C determination\",\"authors\":\"Afsaneh Mousa Pour , Zahra Garkani-Nejad , Hadi Mahmoudi-Moghaddam\",\"doi\":\"10.1016/j.bioelechem.2025.109111\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>In this study, plate-like Eu<sup>3+</sup>-doped MoS₂ nanoparticles (PL-Eu<sup>3+</sup>-doped MoS₂ NPs) were successfully synthesized via a simple hydrothermal method and characterized using energy-dispersive X-ray spectroscopy (EDX), field-emission scanning electron microscopy (FESEM), and X-ray diffraction (XRD). Subsequently, a novel electrochemical DNA biosensor was developed by modifying pencil graphite electrodes (PGEs) with the synthesized nanocomposite and polypyrrole (PPy) for the sensitive detection of mitomycin C (MC). The interaction between double-stranded DNA (ds-DNA) and MC was thoroughly investigated using differential pulse voltammetry (DPV), spectrophotometry, and molecular docking methods. The decrease in the oxidation peak currents of adenine base in an acetate buffer solution (pH 4.80) was employed as a reliable indicator of the interaction event. The constructed biosensor (ds-DNA/PL-Eu<sup>3+</sup>-doped MoS₂/PGE) exhibited a linear response toward MC concentrations ranging from 1 to 75 μg/mL, achieving a low detection limit of 0.8 μg/mL. The sensor's performance was further validated in real sample analyses, demonstrating excellent recovery values between 97 % and 104 %, thereby confirming its potential for practical applications in biomedical and environmental monitoring. UV/Vis spectroscopic data combined with molecular docking analyses indicated substantial interaction between mitomycin and DNA, with evidence supporting groove binding as the predominant binding mode.</div></div>\",\"PeriodicalId\":252,\"journal\":{\"name\":\"Bioelectrochemistry\",\"volume\":\"168 \",\"pages\":\"Article 109111\"},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2025-09-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioelectrochemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1567539425002142\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioelectrochemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1567539425002142","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Development of a highly efficient DNA-based biosensor using a functionalized MoS₂/polypyrrole nanocomposite for mitomycin C determination
In this study, plate-like Eu3+-doped MoS₂ nanoparticles (PL-Eu3+-doped MoS₂ NPs) were successfully synthesized via a simple hydrothermal method and characterized using energy-dispersive X-ray spectroscopy (EDX), field-emission scanning electron microscopy (FESEM), and X-ray diffraction (XRD). Subsequently, a novel electrochemical DNA biosensor was developed by modifying pencil graphite electrodes (PGEs) with the synthesized nanocomposite and polypyrrole (PPy) for the sensitive detection of mitomycin C (MC). The interaction between double-stranded DNA (ds-DNA) and MC was thoroughly investigated using differential pulse voltammetry (DPV), spectrophotometry, and molecular docking methods. The decrease in the oxidation peak currents of adenine base in an acetate buffer solution (pH 4.80) was employed as a reliable indicator of the interaction event. The constructed biosensor (ds-DNA/PL-Eu3+-doped MoS₂/PGE) exhibited a linear response toward MC concentrations ranging from 1 to 75 μg/mL, achieving a low detection limit of 0.8 μg/mL. The sensor's performance was further validated in real sample analyses, demonstrating excellent recovery values between 97 % and 104 %, thereby confirming its potential for practical applications in biomedical and environmental monitoring. UV/Vis spectroscopic data combined with molecular docking analyses indicated substantial interaction between mitomycin and DNA, with evidence supporting groove binding as the predominant binding mode.
期刊介绍:
An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry
Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of:
• Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction.
• Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms)
• Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes)
• Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion)
• Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair).
• Organization and use of arrays in-vitro and in-vivo, including as part of feedback control.
• Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.