680 nm和850 nm发光二极管光生物调节治疗人脑内皮细胞分化的研究进展

IF 2.6 4区 医学 Q2 PHARMACOLOGY & PHARMACY
Hossein Chamkouri, Lei Chen
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引用次数: 0

摘要

光生物调节(PBM)已成为一种有前途的治疗方法,用于调节细胞行为和改善健康结果,特别是在血管健康的背景下。尽管人们对PBM越来越感兴趣,但在了解特定波长(如680 nm和850 nm)如何影响内皮细胞功能方面存在关键差距。虽然已知光对细胞活力和线粒体功能的一般影响,但PBM对内皮细胞影响的确切机制尚不清楚,这限制了PBM治疗血管功能障碍方案的优化。在本研究中,研究了PBM对内皮细胞的影响,使用峰值为680和850 nm,全宽为17.5和25.1 nm的光,分别评估了细胞活力、线粒体活性、活性氧(ROS)产生、钙通量(Ca2+)和经上皮电阻(TEER)。这些发现表明,PBM暴露增强线粒体功能,减少氧化应激,调节钙信号,所有这些都有助于内皮屏障完整性的改变。这些结果突出了PBM作为增强内皮细胞功能和解决内皮功能障碍的新治疗策略的潜力,为血管健康的未来研究和临床应用开辟了新的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Advancement of Photobiomodulation Therapy Using 680 and 850 nm Light-Emitting Diodes for the Differentiation of Human Brain Endothelial Cells

Advancement of Photobiomodulation Therapy Using 680 and 850 nm Light-Emitting Diodes for the Differentiation of Human Brain Endothelial Cells

Advancement of Photobiomodulation Therapy Using 680 and 850 nm Light-Emitting Diodes for the Differentiation of Human Brain Endothelial Cells

Advancement of Photobiomodulation Therapy Using 680 and 850 nm Light-Emitting Diodes for the Differentiation of Human Brain Endothelial Cells

Advancement of Photobiomodulation Therapy Using 680 and 850 nm Light-Emitting Diodes for the Differentiation of Human Brain Endothelial Cells

Photobiomodulation (PBM) has emerged as a promising therapeutic approach for modulating cellular behavior and improving health outcomes, particularly in the context of vascular health. Despite growing interest in PBM, a key gap exists in understanding how specific wavelengths, such as 680 and 850 nm, affect endothelial cell function. While light's general effects on cell viability and mitochondrial function are known, the precise mechanisms underlying PBM's influence on endothelial cells remain unclear, limiting the optimization of PBM protocols for vascular dysfunction. In this study, the effects of PBM on endothelial cells are investigated using the light peaked at 680 and 850 nm with full width at half maximum (FWHM) about 17.5 and 25.1 nm, respectively, assessing cell viability, mitochondrial activity, reactive oxygen species (ROS) production, calcium flux (Ca2+), and transepithelial electrical resistance (TEER). These findings demonstrate that PBM exposure enhances mitochondrial function, reduces oxidative stress, and modulates calcium signaling, all of which contribute to changes in endothelial barrier integrity. These results highlight the potential of PBM as a novel therapeutic strategy for enhancing endothelial cell function and addressing endothelial dysfunction, opening new avenues for future research and clinical applications in vascular health.

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来源期刊
Advanced Therapeutics
Advanced Therapeutics Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
7.10
自引率
2.20%
发文量
130
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