改性高亲和适配体构建的电化学传感器检测孔雀石绿

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-09-21 DOI:10.1007/s00604-025-07506-1

Zanlin Chen, Haiming Li, Yian Chen, Shuangyan Han

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引用次数: 0

摘要

利用分子对接的方法开发了高亲和力的适配体，建立了检测孔雀石绿（MG）的电化学适配体生物传感器。MG-15适配体由MG-36的合理截断得到，其解离常数（Kd）为45.4 \(\pm\) 17.6 μM。通过完全突变MG-15的结合位点实现进一步的增强，获得了更高亲和力的适配体（MG-15- ggt）， Kd值较低，为2.71 \(\pm\) 1.95 μM。该传感器的检出限（LOD）为2.74 pM，具有良好的特异性，回收率为87.1 ～ 111.5%. Consequently, electrochemical biosensors utilizing modified aptamers hold great promise for applications in food safety.Graphical Abstract

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Detection of malachite green by electrochemical sensor constructed from modified high-affinity aptamer

A high-affinity aptamer was developed using a molecular docking approach, leading to the establishment of an electrochemical aptamer biosensor for the detection of malachite green (MG). The aptamer designated as MG-15 was derived from a rational truncation of MG-36, yielding a dissociation constant (K_d) of 45.4 \(\pm\) 17.6 μM. Further enhancements were achieved by completely mutating the binding site of MG-15, resulting in a higher affinity aptamer (MG-15-GGT) with a lower K_d of 2.71 \(\pm\) 1.95 μM. This sensor exhibited a limit of detection (LOD) value of 2.74 pM and demonstrated excellent specificity, with recoveries ranging from 87.1 to 111.5%. Consequently, electrochemical biosensors utilizing modified aptamers hold great promise for applications in food safety.

Graphical Abstract

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.