{"title":"香草婆罗洲罗尔夫的植物化学分析和抗氧化潜力的豆类-一种罕见的,地方性的,和威胁的阿萨姆邦,印度的兰花","authors":"Sneha Hasnu , Bikash Kumar Kundu , Beauty Rani Basumatary , Dikshit Goswami , Sankar Som , Mehzabin Rehman , Bhaben Tanti","doi":"10.1016/j.prenap.2025.100372","DOIUrl":null,"url":null,"abstract":"<div><div><em>Vanilla borneensis</em> Rolfe, a rare, endemic, and critically endangered orchid native to Assam, India, holds substantial ecological and ethnobotanical value. However, its phytochemical and pharmacological attributes remain largely uncharacterized. In light of increasing efforts to conserve endangered species through value-added utilization, this study aimed to investigate the phytochemical composition and antioxidant potential of <em>V. borneensis</em> beans to inform both conservation and bioprospecting strategies. Beans were obtained through controlled hand pollination and subjected to a standardized curing protocol. Methanol and ethanol extracts were analyzed using qualitative and quantitative phytochemical assays, revealing the presence of flavonoids, phenols, diterpenoids, coumarins, alkaloids, sugars, glycosides, phytosterols, and trace tannins. Quantitative profiling indicated higher flavonoid, tannin, and alkaloid content in ethanol extracts. Antioxidant evaluation using DPPH, ABTS, H<sub>2</sub>O<sub>2</sub> scavenging, and total antioxidant capacity assays showed superior activity in ethanol extracts, with IC<sub>50</sub> values of 86.69 ppm (ethanol) and 99.26 ppm (methanol) in DPPH assays. Notably, both extracts exhibited stronger H<sub>2</sub>O<sub>2</sub> scavenging activity compared to ascorbic acid. GC–MS analysis identified 11 major volatiles in ethanol and 17 in methanol extracts, including food- and fragrance-relevant compounds such as N-ethylacetamide, 1,8-cineole, and methyl esters of long-chain fatty acids. Although vanillin was absent, the detected compounds possess industrial applicability in food, cosmetics, and nutraceuticals. These findings underscore the pharmacological and conservation relevance of <em>V. borneensis</em>, offering a scientific foundation for its sustainable utilization and protection.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"9 ","pages":"Article 100372"},"PeriodicalIF":0.0000,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phytochemical profiling and antioxidant potential in the beans of Vanilla borneensis Rolfe – a rare, endemic, and threatened Orchid of Assam, India\",\"authors\":\"Sneha Hasnu , Bikash Kumar Kundu , Beauty Rani Basumatary , Dikshit Goswami , Sankar Som , Mehzabin Rehman , Bhaben Tanti\",\"doi\":\"10.1016/j.prenap.2025.100372\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>Vanilla borneensis</em> Rolfe, a rare, endemic, and critically endangered orchid native to Assam, India, holds substantial ecological and ethnobotanical value. However, its phytochemical and pharmacological attributes remain largely uncharacterized. In light of increasing efforts to conserve endangered species through value-added utilization, this study aimed to investigate the phytochemical composition and antioxidant potential of <em>V. borneensis</em> beans to inform both conservation and bioprospecting strategies. Beans were obtained through controlled hand pollination and subjected to a standardized curing protocol. Methanol and ethanol extracts were analyzed using qualitative and quantitative phytochemical assays, revealing the presence of flavonoids, phenols, diterpenoids, coumarins, alkaloids, sugars, glycosides, phytosterols, and trace tannins. Quantitative profiling indicated higher flavonoid, tannin, and alkaloid content in ethanol extracts. Antioxidant evaluation using DPPH, ABTS, H<sub>2</sub>O<sub>2</sub> scavenging, and total antioxidant capacity assays showed superior activity in ethanol extracts, with IC<sub>50</sub> values of 86.69 ppm (ethanol) and 99.26 ppm (methanol) in DPPH assays. Notably, both extracts exhibited stronger H<sub>2</sub>O<sub>2</sub> scavenging activity compared to ascorbic acid. GC–MS analysis identified 11 major volatiles in ethanol and 17 in methanol extracts, including food- and fragrance-relevant compounds such as N-ethylacetamide, 1,8-cineole, and methyl esters of long-chain fatty acids. Although vanillin was absent, the detected compounds possess industrial applicability in food, cosmetics, and nutraceuticals. These findings underscore the pharmacological and conservation relevance of <em>V. borneensis</em>, offering a scientific foundation for its sustainable utilization and protection.</div></div>\",\"PeriodicalId\":101014,\"journal\":{\"name\":\"Pharmacological Research - Natural Products\",\"volume\":\"9 \",\"pages\":\"Article 100372\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-09-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmacological Research - Natural Products\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2950199725002320\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacological Research - Natural Products","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2950199725002320","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Phytochemical profiling and antioxidant potential in the beans of Vanilla borneensis Rolfe – a rare, endemic, and threatened Orchid of Assam, India
Vanilla borneensis Rolfe, a rare, endemic, and critically endangered orchid native to Assam, India, holds substantial ecological and ethnobotanical value. However, its phytochemical and pharmacological attributes remain largely uncharacterized. In light of increasing efforts to conserve endangered species through value-added utilization, this study aimed to investigate the phytochemical composition and antioxidant potential of V. borneensis beans to inform both conservation and bioprospecting strategies. Beans were obtained through controlled hand pollination and subjected to a standardized curing protocol. Methanol and ethanol extracts were analyzed using qualitative and quantitative phytochemical assays, revealing the presence of flavonoids, phenols, diterpenoids, coumarins, alkaloids, sugars, glycosides, phytosterols, and trace tannins. Quantitative profiling indicated higher flavonoid, tannin, and alkaloid content in ethanol extracts. Antioxidant evaluation using DPPH, ABTS, H2O2 scavenging, and total antioxidant capacity assays showed superior activity in ethanol extracts, with IC50 values of 86.69 ppm (ethanol) and 99.26 ppm (methanol) in DPPH assays. Notably, both extracts exhibited stronger H2O2 scavenging activity compared to ascorbic acid. GC–MS analysis identified 11 major volatiles in ethanol and 17 in methanol extracts, including food- and fragrance-relevant compounds such as N-ethylacetamide, 1,8-cineole, and methyl esters of long-chain fatty acids. Although vanillin was absent, the detected compounds possess industrial applicability in food, cosmetics, and nutraceuticals. These findings underscore the pharmacological and conservation relevance of V. borneensis, offering a scientific foundation for its sustainable utilization and protection.