香草婆罗洲罗尔夫的植物化学分析和抗氧化潜力的豆类-一种罕见的,地方性的,和威胁的阿萨姆邦,印度的兰花

Sneha Hasnu , Bikash Kumar Kundu , Beauty Rani Basumatary , Dikshit Goswami , Sankar Som , Mehzabin Rehman , Bhaben Tanti
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引用次数: 0

摘要

香草婆罗洲罗尔夫是一种稀有的、地方性的、极度濒危的兰花,原产于印度阿萨姆邦,具有重要的生态和民族植物学价值。然而,它的植物化学和药理学属性在很大程度上仍然是未知的。鉴于通过增值利用来保护濒危物种的努力越来越多,本研究旨在研究婆罗洲豆的植物化学成分和抗氧化潜力,为保护和生物勘探策略提供依据。豆子是通过控制手授粉获得的,并经过标准化的养护程序。用定性和定量的植物化学方法分析甲醇和乙醇提取物,揭示了黄酮类、酚类、二萜、香豆素、生物碱、糖、糖苷、植物甾醇和微量单宁的存在。定量分析表明乙醇提取物的类黄酮、单宁和生物碱含量较高。通过DPPH、ABTS、H2O2清除和总抗氧化能力的测定,表明乙醇提取物具有较好的抗氧化活性,其IC50值分别为86.69 ppm(乙醇)和99.26 ppm(甲醇)。值得注意的是,与抗坏血酸相比,两种提取物都表现出更强的H2O2清除活性。GC-MS分析确定了乙醇中的11种主要挥发物和甲醇提取物中的17种主要挥发物,包括与食品和香料相关的化合物,如n -乙基乙酰胺、1,8-桉树脑和长链脂肪酸甲酯。虽然没有香兰素,但检测到的化合物在食品、化妆品和营养药品中具有工业适用性。这些发现强调了龙骨草的药理和保护意义,为其可持续利用和保护提供了科学依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Phytochemical profiling and antioxidant potential in the beans of Vanilla borneensis Rolfe – a rare, endemic, and threatened Orchid of Assam, India

Phytochemical profiling and antioxidant potential in the beans of Vanilla borneensis Rolfe – a rare, endemic, and threatened Orchid of Assam, India
Vanilla borneensis Rolfe, a rare, endemic, and critically endangered orchid native to Assam, India, holds substantial ecological and ethnobotanical value. However, its phytochemical and pharmacological attributes remain largely uncharacterized. In light of increasing efforts to conserve endangered species through value-added utilization, this study aimed to investigate the phytochemical composition and antioxidant potential of V. borneensis beans to inform both conservation and bioprospecting strategies. Beans were obtained through controlled hand pollination and subjected to a standardized curing protocol. Methanol and ethanol extracts were analyzed using qualitative and quantitative phytochemical assays, revealing the presence of flavonoids, phenols, diterpenoids, coumarins, alkaloids, sugars, glycosides, phytosterols, and trace tannins. Quantitative profiling indicated higher flavonoid, tannin, and alkaloid content in ethanol extracts. Antioxidant evaluation using DPPH, ABTS, H2O2 scavenging, and total antioxidant capacity assays showed superior activity in ethanol extracts, with IC50 values of 86.69 ppm (ethanol) and 99.26 ppm (methanol) in DPPH assays. Notably, both extracts exhibited stronger H2O2 scavenging activity compared to ascorbic acid. GC–MS analysis identified 11 major volatiles in ethanol and 17 in methanol extracts, including food- and fragrance-relevant compounds such as N-ethylacetamide, 1,8-cineole, and methyl esters of long-chain fatty acids. Although vanillin was absent, the detected compounds possess industrial applicability in food, cosmetics, and nutraceuticals. These findings underscore the pharmacological and conservation relevance of V. borneensis, offering a scientific foundation for its sustainable utilization and protection.
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