Paula Bucci , Danilo Cantero , Andrea Casas , Enrique Marcos , Enkeledo Menalla , Raúl Muñoz
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The novelty lies in the combination of a continuous hydrothermal pretreatment reactor with meta-transcriptomic analysis of the anaerobic digestion process, enabling a direct correlation between operational performance and microbial functional shifts. Hydrothermal pretreatment was applied to partially hydrolyze cellulose and hemicellulose into fermentable sugars, improving biodegradability while minimizing the formation of inhibitory compounds. Results showed that hydrothermally pretreated BSG supported methane productions over 1500 mL CH<sub>4</sub>/L_reactor·day, markedly higher than the 100–500 mL CH<sub>4</sub>/L_reactor·day observed for untreated BSG. Volatile solids degradation efficiency improved by 30 %, while methane content in biogas increased from 30 % to 65 %. Genomic analysis of the microbial consortium revealed enhanced activity of methanogenic archaea and fermentative bacteria associated with the increased methane production. 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引用次数: 0
摘要
啤酒公司的废谷物(BSG)是酿酒业的副产品,面临着重大的废物管理挑战,但作为通过厌氧消化(AD)生产沼气的底物,它具有潜力。然而,其木质纤维素的复杂性限制了微生物降解和甲烷产量。在我们课课组之前的研究中,探讨了BSG的各种治疗策略,包括旨在回收高价值化合物的方法。在此基础上,本工作评估了水热预处理通过AD提高BSG甲烷产量的潜力。其新颖之处在于将连续水热预处理反应器与厌氧消化过程的元转录组学分析相结合,从而实现了操作性能与微生物功能变化之间的直接关联。采用水热预处理将纤维素和半纤维素部分水解为可发酵糖,提高了生物降解性,同时最大限度地减少了抑制性化合物的形成。结果表明,水热预处理后的BSG甲烷产量超过1500 mL CH4/ l_反应器·d,显著高于未处理BSG的100-500 mL CH4/ l_反应器·d。挥发性固体降解效率提高30%,沼气中甲烷含量由30%提高到65%。微生物联合体的基因组分析显示,产甲烷古细菌和发酵细菌的活性增强与甲烷产量增加有关。这种综合方法不仅更有效地破坏了木质纤维素屏障,而且还提供了对微生物功能的更深入了解,加强了水热预处理作为提高沼气产量和推进可持续废物转化能源解决方案的可行策略。
Hydrothermal pretreatment of brewer's spent grain: A pathway to sustainable biogas production and waste valorization
Brewer's spent grain (BSG), a by-product of the brewing industry, faces significant waste management challenges but holds potential as a substrate for biogas production via anaerobic digestion (AD). However, its lignocellulosic complexity limits microbial degradation and methane yield. In previous studies by our research group, various treatment strategies for BSG were explored, including approaches aimed at recovering high-value compounds. Building upon this foundation, the present work evaluates the potential of hydrothermal pretreatment to enhance methane production from BSG via AD. The novelty lies in the combination of a continuous hydrothermal pretreatment reactor with meta-transcriptomic analysis of the anaerobic digestion process, enabling a direct correlation between operational performance and microbial functional shifts. Hydrothermal pretreatment was applied to partially hydrolyze cellulose and hemicellulose into fermentable sugars, improving biodegradability while minimizing the formation of inhibitory compounds. Results showed that hydrothermally pretreated BSG supported methane productions over 1500 mL CH4/L_reactor·day, markedly higher than the 100–500 mL CH4/L_reactor·day observed for untreated BSG. Volatile solids degradation efficiency improved by 30 %, while methane content in biogas increased from 30 % to 65 %. Genomic analysis of the microbial consortium revealed enhanced activity of methanogenic archaea and fermentative bacteria associated with the increased methane production. This integrated approach not only disrupts lignocellulosic barriers more effectively but also provides deeper insights into microbial functionality, reinforcing hydrothermal pretreatment as a viable strategy for boosting biogas yield and advancing sustainable waste-to-energy solutions.
期刊介绍:
Biomass & Bioenergy is an international journal publishing original research papers and short communications, review articles and case studies on biological resources, chemical and biological processes, and biomass products for new renewable sources of energy and materials.
The scope of the journal extends to the environmental, management and economic aspects of biomass and bioenergy.
Key areas covered by the journal:
• Biomass: sources, energy crop production processes, genetic improvements, composition. Please note that research on these biomass subjects must be linked directly to bioenergy generation.
• Biological Residues: residues/rests from agricultural production, forestry and plantations (palm, sugar etc), processing industries, and municipal sources (MSW). Papers on the use of biomass residues through innovative processes/technological novelty and/or consideration of feedstock/system sustainability (or unsustainability) are welcomed. However waste treatment processes and pollution control or mitigation which are only tangentially related to bioenergy are not in the scope of the journal, as they are more suited to publications in the environmental arena. Papers that describe conventional waste streams (ie well described in existing literature) that do not empirically address ''new'' added value from the process are not suitable for submission to the journal.
• Bioenergy Processes: fermentations, thermochemical conversions, liquid and gaseous fuels, and petrochemical substitutes
• Bioenergy Utilization: direct combustion, gasification, electricity production, chemical processes, and by-product remediation
• Biomass and the Environment: carbon cycle, the net energy efficiency of bioenergy systems, assessment of sustainability, and biodiversity issues.