{"title":"组学分析揭示了马地黄抗除草剂的关键基因","authors":"Yidi Guan, Liru Liu, Zhi Xiong, Caiwen Li, Chunyuan Liu, Yuan Sun, Mingshan Ji","doi":"10.1016/j.pestbp.2025.106693","DOIUrl":null,"url":null,"abstract":"<div><div><em>Digitaria sanguinalis</em> (L.) Scop is recognized as one of the most problematic agricultural weeds, with chemical control remaining a crucial management strategy. However, increased selection pressures have led to the emergence of resistant populations that reproduce and establish themselves as dominant communities, thereby severely jeopardizing crop yields. It is especially crucial to reveal the resistance mechanism of <em>D.Sanguinalis</em>, and the lack of weed histology resources has always been an obstacle to the study of resistance mechanism, nowadays, with the development of histology technology, the combination of multi-omics applied to the identification of weed resistance genes is becoming more and more perfect. In our previous study, we have preliminarily demonstrated that the resistance of <em>D.Sanguinalis</em> to ALS inhibitors is related to the increase of P450 enzyme activity. Here, we employed single-molecule real-time (SMRT) sequencing technology to obtain full-length transcripts of <em>D. sanguinalis.</em> Using DIA proteomics, we identified upregulated herbicide-metabolizing proteins, which were validated via PRM analysis. By integrating the transcriptomic and proteomic results, we identified <em>CYP709B2</em> and <em>CYP74B2</em> as key effector genes that mediate resistance in <em>D. sanguinalis</em>. We elucidated the resistance patterns and specific genes associated with <em>D. sanguinalis</em>, thereby enriching the bioinformatics resources available for this species and providing a foundation for herbicide-resistant weed management.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"215 ","pages":"Article 106693"},"PeriodicalIF":4.0000,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Omics analysis reveals key genes mediating herbicide resistance in Digitaria sanguinalis\",\"authors\":\"Yidi Guan, Liru Liu, Zhi Xiong, Caiwen Li, Chunyuan Liu, Yuan Sun, Mingshan Ji\",\"doi\":\"10.1016/j.pestbp.2025.106693\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>Digitaria sanguinalis</em> (L.) Scop is recognized as one of the most problematic agricultural weeds, with chemical control remaining a crucial management strategy. However, increased selection pressures have led to the emergence of resistant populations that reproduce and establish themselves as dominant communities, thereby severely jeopardizing crop yields. It is especially crucial to reveal the resistance mechanism of <em>D.Sanguinalis</em>, and the lack of weed histology resources has always been an obstacle to the study of resistance mechanism, nowadays, with the development of histology technology, the combination of multi-omics applied to the identification of weed resistance genes is becoming more and more perfect. In our previous study, we have preliminarily demonstrated that the resistance of <em>D.Sanguinalis</em> to ALS inhibitors is related to the increase of P450 enzyme activity. Here, we employed single-molecule real-time (SMRT) sequencing technology to obtain full-length transcripts of <em>D. sanguinalis.</em> Using DIA proteomics, we identified upregulated herbicide-metabolizing proteins, which were validated via PRM analysis. By integrating the transcriptomic and proteomic results, we identified <em>CYP709B2</em> and <em>CYP74B2</em> as key effector genes that mediate resistance in <em>D. sanguinalis</em>. We elucidated the resistance patterns and specific genes associated with <em>D. sanguinalis</em>, thereby enriching the bioinformatics resources available for this species and providing a foundation for herbicide-resistant weed management.</div></div>\",\"PeriodicalId\":19828,\"journal\":{\"name\":\"Pesticide Biochemistry and Physiology\",\"volume\":\"215 \",\"pages\":\"Article 106693\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2025-09-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pesticide Biochemistry and Physiology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0048357525004067\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pesticide Biochemistry and Physiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0048357525004067","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Digitaria sanguinalis (L.) Scop is recognized as one of the most problematic agricultural weeds, with chemical control remaining a crucial management strategy. However, increased selection pressures have led to the emergence of resistant populations that reproduce and establish themselves as dominant communities, thereby severely jeopardizing crop yields. It is especially crucial to reveal the resistance mechanism of D.Sanguinalis, and the lack of weed histology resources has always been an obstacle to the study of resistance mechanism, nowadays, with the development of histology technology, the combination of multi-omics applied to the identification of weed resistance genes is becoming more and more perfect. In our previous study, we have preliminarily demonstrated that the resistance of D.Sanguinalis to ALS inhibitors is related to the increase of P450 enzyme activity. Here, we employed single-molecule real-time (SMRT) sequencing technology to obtain full-length transcripts of D. sanguinalis. Using DIA proteomics, we identified upregulated herbicide-metabolizing proteins, which were validated via PRM analysis. By integrating the transcriptomic and proteomic results, we identified CYP709B2 and CYP74B2 as key effector genes that mediate resistance in D. sanguinalis. We elucidated the resistance patterns and specific genes associated with D. sanguinalis, thereby enriching the bioinformatics resources available for this species and providing a foundation for herbicide-resistant weed management.
期刊介绍:
Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance.
Research Areas Emphasized Include the Biochemistry and Physiology of:
• Comparative toxicity
• Mode of action
• Pathophysiology
• Plant growth regulators
• Resistance
• Other effects of pesticides on both parasites and hosts.