蛇牙仿生智能反应两阶段AIE含基因微针平台，用于综合光动力感染伤口管理和视觉治疗诊断

IF 18 1区医学 Q1 ENGINEERING, BIOMEDICAL

Bioactive Materials Pub Date : 2025-09-16 DOI:10.1016/j.bioactmat.2025.09.013

Lijuan Chen , Xiaomin Luo , Hao Yang , Ying Liu , Jiamin Zhang , Qian Zhang , Fengqian Yang , Xinhua Liu , Huie Jiang

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引用次数: 0

摘要

有效治疗因临床使用常规抗生素引起的普遍耐药（DR）细菌感染伤口仍然是一项重大挑战。本研究创新性地提出了一种结合新兴聚集诱导发光源（AIE-gen）和微针技术的智能释药、光动力治疗和视觉治疗诊断策略，用于晚期感染创面的治疗。该策略基于具有双层结构的智能响应两阶段含aie -原的微针平台（AIE-film/TS-MN）。最上层包括按需ph响应的aie -光敏剂（AFPP）基薄膜（aie -薄膜）。这一层的设计目的是为了精确释放活性氧（ROS）或硝基烃，以响应DR细菌中的β-内酰胺酶。基底层是一个两阶段微针（TS-MN）贴片，具有“核-壳”结构，生物灵感来自蛇牙，通过装载铁-卡比西林框架（Fe-MOF）的甲壳性明胶提供出色的抗迁移起源和酸反应性药物释放特性。aie膜中的AFPP和硝基蛋白对酸性伤口微环境和β-内酰胺酶反应准确，颜色分别由黄色和绿色变为橙色和红色。这表明AIE-film/TS-MN作为一种新型的生物传感器具有巨大的潜力，可以通过智能手机分析半定量地记录伤口部位的颜色变化，从视觉上实现明显的感染伤口诊断/评估，从而促进治疗效果。此外，智能多重药物释放协同消除耐药细菌，包括从Fe-MOF和激活afpp的ROS中的卡比西林。全面的体外和体内实验证实，远程医疗ae -film/TS-MN为主动感染伤口管理提供了先进的综合治疗效果。此外，它减少了抗生素的滥用，并为视觉医学诊断和治疗干预提供了一种创新和有前途的战略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Snake-fangs-bioinspired intelligently-responsive two-stage AIE gen-containing microneedle-based platform for integrated photodynamic infected-wound management and visual therapeutic diagnosis

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Snake-fangs-bioinspired intelligently-responsive two-stage AIE gen-containing microneedle-based platform for integrated photodynamic infected-wound management and visual therapeutic diagnosis

The efficient treatment of prevalent drug-resistant (DR) bacteria-infected wounds caused by the clinical use of conventional antibiotics remains a significant challenge. Here, a well-integrated intelligent drug-releasing, photodynamic therapy, and visual therapeutic diagnosis strategy combining burgeoning aggregation-induced emission luminogen (AIE-gen) and microneedling technology is innovatively proposed for advanced infected-wound management. This strategy is based on an intelligently responsive two-stage AIE-gen-containing microneedle-based platform (AIE-film/TS-MN) with a double-layer structure. The uppermost layer comprises an on-demand pH-responsive AIE-photosensitizer (AFPP)-based film (AIE-film). This layer is designed to achieve the precise release of reactive oxygen species (ROS) or nitrocefin, which responds to β-lactamase in DR bacteria. The basal layer is a two-stage microneedle (TS-MN) patch with a “core-shell” structure bio-inspired by snake fangs, providing outstanding anti-migratory origins and acid-responsive drug-releasing properties through crustaceous gelatin loaded with Fe-carbenicillin frameworks (Fe-MOF). AFPP and nitrocefin in AIE-film accurately respond to acidic wound-microenvironment and β-lactamase, colorfully changing from yellow and green to orange and red, respectively. This indicates that AIE-film/TS-MN exhibits significant potential as a novel biosensor to record the color-change semiquantitatively at wound-sites through smartphone analysis, visually achieving significantly infected wound-diagnosis/-assessment to facilitate the therapeutic efficacy. Additionally, the smart multiple drug-releasing synergistically eliminates DR-bacteria, including carbenicillin from Fe-MOF and AFPP-activating ROS. Comprehensive in vitro and in vivo experiments confirm that the telemedicine AIE-film/TS-MN offers advanced integrated therapeutic effects for active infected wound management. Additionally, it reduces antibiotic abuse and provides an innovative and promising strategy for visual medical diagnostics and therapeutic interventions.

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来源期刊

Bioactive Materials Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

28.00

自引率

6.30%

发文量

436

审稿时长

20 days

期刊介绍： Bioactive Materials is a peer-reviewed research publication that focuses on advancements in bioactive materials. The journal accepts research papers, reviews, and rapid communications in the field of next-generation biomaterials that interact with cells, tissues, and organs in various living organisms. The primary goal of Bioactive Materials is to promote the science and engineering of biomaterials that exhibit adaptiveness to the biological environment. These materials are specifically designed to stimulate or direct appropriate cell and tissue responses or regulate interactions with microorganisms. The journal covers a wide range of bioactive materials, including those that are engineered or designed in terms of their physical form (e.g. particulate, fiber), topology (e.g. porosity, surface roughness), or dimensions (ranging from macro to nano-scales). Contributions are sought from the following categories of bioactive materials: Bioactive metals and alloys Bioactive inorganics: ceramics, glasses, and carbon-based materials Bioactive polymers and gels Bioactive materials derived from natural sources Bioactive composites These materials find applications in human and veterinary medicine, such as implants, tissue engineering scaffolds, cell/drug/gene carriers, as well as imaging and sensing devices.