利用同手性金属-有机框架结合l-二苯丙氨酸靶向位点的超灵敏电化学检测淀粉样蛋白-β肽。

IF 9.1 1区 化学 Q1 CHEMISTRY, ANALYTICAL
Rui-Xuan Li,Xin Shang,Pei-Qi Shen,Yu-Feng Zhu,En-Qing Gao,Qi Yue
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引用次数: 0

摘要

淀粉样蛋白-β肽(Aβ)检测具有较高的灵敏度和准确性,可为阿尔茨海默病(AD)的早期诊断、疾病进展监测和有效治疗提供有力支持。提高检测材料对Aβ的特异性和亲和力是检测Aβ的关键。本文首次建立了基于未修饰的同手性MOF的电化学传感器Zn-BPIleBp,用于检测Aβ1-40,超低检出限为1.7 pM (7.36 pg/mL)。Zn-BPIleBp传感器对a β1-40核心识别基元对应的二苯丙氨酸(PhePhe)具有高效的对端选择性和超灵敏度,其峰值电流比(IL/ID)为2.78,电位差(EL - ED)为140 mV, l-PhePhe的超低检出限为34 fM。该传感器已成功应用于消旋混合物、人工脑脊液(aCSF)和胎牛血清(FBS)中l-PhePhe和a - β1-40的超灵敏定量。结构和光谱分析表明,同手性MOF对Aβ1-40具有很高的敏感性和亲和力,而不涉及任何生物分子修饰,如抗体或适体,这源于框架与Aβ1-40的结构、疏水和手性匹配。该传感器为a - β1-40提供了一种快速、高稳定、重复性好、超灵敏、准确的检测方法,在AD的临床应用中具有很大的潜力。这项工作也为使用mof作为传感材料设计传感平台以及将其功能和应用扩展到生物和医学分析领域开辟了新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ultrasensitive Electrochemical Detection of Amyloid-β Peptide Using a Homochiral Metal-Organic Framework Binding to the l-Diphenylalanine Targeting Site.
The high sensitivity and accuracy of amyloid-β peptide (Aβ) detection could provide strong support for early diagnosis, monitoring of disease progression, and effective treatment of Alzheimer's disease (AD). Improving the specificity and affinity of sensing materials for Aβ is key to detecting Aβ. Herein, an electrochemical sensor based on an unmodified homochiral MOF, Zn-BPIleBp, was developed for the first time for the detection of Aβ1-40, with an ultralow detection limit of 1.7 pM (7.36 pg/mL). The Zn-BPIleBp sensor also displays high-efficiency enantioselectivity and ultrasensitivity in identifying diphenylalanine (PhePhe) corresponding to the core recognition motif of Aβ1-40, with a high peak current ratio (IL/ID) of 2.78, large potential difference (EL - ED) of 140 mV, and ultralow detection limit of 34 fM for l-PhePhe. The sensor has been successfully applied for the ultrasensitive quantification of l-PhePhe and Aβ1-40 in racemic mixtures, artificial cerebrospinal fluid (aCSF) and fetal bovine serum (FBS). According to structural and spectral analysis, the high sensitivity and affinity of homochiral MOF toward Aβ1-40, without the involvement of any biomolecular modifications such as antibodies or aptamers, stem from the structural, hydrophobic, and chiral matching between the framework and Aβ1-40. This sensor proffers a fast, highly stable, reproducible, ultrasensitive, and accurate detection method for Aβ1-40, demonstrating great potential in the clinical application of AD. This work also opens up new perspectives for designing sensing platforms using MOFs as sensing materials and expanding their functionality and applications to the fields of biological and medical analysis.
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来源期刊
ACS Sensors
ACS Sensors Chemical Engineering-Bioengineering
CiteScore
14.50
自引率
3.40%
发文量
372
期刊介绍: ACS Sensors is a peer-reviewed research journal that focuses on the dissemination of new and original knowledge in the field of sensor science, particularly those that selectively sense chemical or biological species or processes. The journal covers a broad range of topics, including but not limited to biosensors, chemical sensors, gas sensors, intracellular sensors, single molecule sensors, cell chips, and microfluidic devices. It aims to publish articles that address conceptual advances in sensing technology applicable to various types of analytes or application papers that report on the use of existing sensing concepts in new ways or for new analytes.
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