Yujie Wang , Yanfan Cui , Yixuan Li , Tao Luo , Jiyan Li
{"title":"不同类型的纳米塑料在与精液相关的浓度下对人类精子产生不同程度的毒性。","authors":"Yujie Wang , Yanfan Cui , Yixuan Li , Tao Luo , Jiyan Li","doi":"10.1016/j.reprotox.2025.109064","DOIUrl":null,"url":null,"abstract":"<div><div>Previous research on the toxicity of nanoplastics (NPs) on male reproduction have centered on polystyrene (PS), a diverse range of NPs types has been identified in human semen and testes, warranting further investigation. In the present study, we employed pyrolysis gas chromatography-mass spectrometry (Py-GCMS) to quantify the presence of multiple NPs in human semen. Subsequently, we assessed the impact of various NPs at semen-related concentrations on human sperm functions and physiological parameters. Polypropylene (PP, 0.19–33.85 μg/mL), polyethylene (PE, 3.53–865.55 μg/mL), and polyethylene terephthalate (PET, 305.74 μg/mL) were detected in human semen. Exposure to PE at concentrations of 0.5 and 1 mg/mL significantly reduced sperm viability, motility, penetration ability, and mitochondrial membrane potential (MMP), while also inducing oxidative stress. At 1 mg/mL, PET decreased sperm motility, penetration ability and MMP, though it did not affect viability or reactive oxygen species (ROS) levels. In contrast, PP exhibited no adverse effects on sperm functions and physiological parameters at semen-related concentrations. Our findings demonstrate that different types of NPs exert varying degrees of toxicity on human sperm at semen-related concentrations, with PE exhibiting the highest toxicity and PP showing no observable toxicity. Moreover, exposure to mixture of NPs more accurately reflects human daily exposure scenarios. We evaluated the effects of mixed NPs on sperm function and observed that, with increasing co-incubation time, the NPs mixture induced more pronounced impairments in sperm viability and other parameters compared to exposure to any single type of NPs.</div></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":"138 ","pages":"Article 109064"},"PeriodicalIF":2.8000,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Different types of nanoplastics exert varying degrees of toxicity on human sperm at semen-related concentrations\",\"authors\":\"Yujie Wang , Yanfan Cui , Yixuan Li , Tao Luo , Jiyan Li\",\"doi\":\"10.1016/j.reprotox.2025.109064\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Previous research on the toxicity of nanoplastics (NPs) on male reproduction have centered on polystyrene (PS), a diverse range of NPs types has been identified in human semen and testes, warranting further investigation. In the present study, we employed pyrolysis gas chromatography-mass spectrometry (Py-GCMS) to quantify the presence of multiple NPs in human semen. Subsequently, we assessed the impact of various NPs at semen-related concentrations on human sperm functions and physiological parameters. Polypropylene (PP, 0.19–33.85 μg/mL), polyethylene (PE, 3.53–865.55 μg/mL), and polyethylene terephthalate (PET, 305.74 μg/mL) were detected in human semen. Exposure to PE at concentrations of 0.5 and 1 mg/mL significantly reduced sperm viability, motility, penetration ability, and mitochondrial membrane potential (MMP), while also inducing oxidative stress. At 1 mg/mL, PET decreased sperm motility, penetration ability and MMP, though it did not affect viability or reactive oxygen species (ROS) levels. In contrast, PP exhibited no adverse effects on sperm functions and physiological parameters at semen-related concentrations. Our findings demonstrate that different types of NPs exert varying degrees of toxicity on human sperm at semen-related concentrations, with PE exhibiting the highest toxicity and PP showing no observable toxicity. Moreover, exposure to mixture of NPs more accurately reflects human daily exposure scenarios. We evaluated the effects of mixed NPs on sperm function and observed that, with increasing co-incubation time, the NPs mixture induced more pronounced impairments in sperm viability and other parameters compared to exposure to any single type of NPs.</div></div>\",\"PeriodicalId\":21137,\"journal\":{\"name\":\"Reproductive toxicology\",\"volume\":\"138 \",\"pages\":\"Article 109064\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2025-09-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Reproductive toxicology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0890623825002357\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"REPRODUCTIVE BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reproductive toxicology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0890623825002357","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"REPRODUCTIVE BIOLOGY","Score":null,"Total":0}
Different types of nanoplastics exert varying degrees of toxicity on human sperm at semen-related concentrations
Previous research on the toxicity of nanoplastics (NPs) on male reproduction have centered on polystyrene (PS), a diverse range of NPs types has been identified in human semen and testes, warranting further investigation. In the present study, we employed pyrolysis gas chromatography-mass spectrometry (Py-GCMS) to quantify the presence of multiple NPs in human semen. Subsequently, we assessed the impact of various NPs at semen-related concentrations on human sperm functions and physiological parameters. Polypropylene (PP, 0.19–33.85 μg/mL), polyethylene (PE, 3.53–865.55 μg/mL), and polyethylene terephthalate (PET, 305.74 μg/mL) were detected in human semen. Exposure to PE at concentrations of 0.5 and 1 mg/mL significantly reduced sperm viability, motility, penetration ability, and mitochondrial membrane potential (MMP), while also inducing oxidative stress. At 1 mg/mL, PET decreased sperm motility, penetration ability and MMP, though it did not affect viability or reactive oxygen species (ROS) levels. In contrast, PP exhibited no adverse effects on sperm functions and physiological parameters at semen-related concentrations. Our findings demonstrate that different types of NPs exert varying degrees of toxicity on human sperm at semen-related concentrations, with PE exhibiting the highest toxicity and PP showing no observable toxicity. Moreover, exposure to mixture of NPs more accurately reflects human daily exposure scenarios. We evaluated the effects of mixed NPs on sperm function and observed that, with increasing co-incubation time, the NPs mixture induced more pronounced impairments in sperm viability and other parameters compared to exposure to any single type of NPs.
期刊介绍:
Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine.
All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.