沙鼠去势后前列腺炎症标志物和组织结构的改变。

IF 1.5 4区 生物学 Q4 CELL BIOLOGY
Nayara Fernanda Costa Castro, Vitor Grigio, Cássia Regina Suzuki Caires, Mariele Ilario Zucão, Sebastião Roberto Taboga, Patrícia Simone Leite Vilamaior
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引用次数: 0

摘要

睾丸切除术引起前列腺上皮细胞萎缩,这是由雄激素剥夺引起的。本研究的目的是评估蒙古沙鼠腹侧前列腺在短时间内雄激素剥夺时组织重塑引起的变化。将成年沙鼠分为对照组和阉割组,分别于切除睾丸后第3天(Ca3d)、第7天(Ca7d)和第14天(Ca14d)实施安乐死(n = 7/组)。对前列腺腹侧叶进行组织学、体视学、形态计量学、血清学和免疫组织化学分析。阉割促进了腹侧前列腺重量的减少。它改变了前列腺组织间室的相对比例,如上皮和非肌肉间质减少,肌肉间质增加。此外,我们观察到Ca3d组中Cd-68阳性细胞的数量增加,这代表了一段以前文献中未报道的蒙古沙鼠雄激素剥夺时期。基质金属蛋白酶-9定量显示Ca7d组与Ca3d组相比降低。此外,Ca14d组肿瘤坏死因子α的频率增加,受睾酮缺乏持续时间的影响。这些发现有助于理解去势后前列腺组织的重塑,并突出了去势后短时间内前列腺微环境的快速变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Alterations in Inflammatory Markers and Tissue Architecture in the Gerbil Prostate Following Castration.

SummaryOrchiectomy induces atrophy of epithelial cells in the prostate gland, stimulated by androgen deprivation. The objective of the current study was to assess the changes arising from tissue remodeling during short periods of androgen deprivation in the ventral prostate of Mongolian gerbils. Adult male gerbils were divided into groups: control and castrated, euthanized on the 3rd (Ca3d), 7th (Ca7d), and 14th (Ca14d) days post-orchiectomy (n = 7/group). The ventral lobe of the prostate was submitted for histological, stereological, morphometric, serological, and immunohistochemical analyses. Castration promoted a reduction in the weight of the ventral prostate. It altered the relative proportion of prostate tissue compartments, such as a decrease in the epithelium and non-muscular stroma and an increase in the muscular stroma. In addition, we observed that the number of Cd-68 positive cells increased in the Ca3d group, which represents a period of androgen deprivation not previously reported in the literature for Mongolian gerbils. Matrix metalloproteinase-9 quantification revealed a decrease in the Ca7d group compared to the Ca3d. In addition, the frequency of Tumor Necrosis Factor alpha increased in the Ca14d group, influenced by the duration of testosterone deficiency. The findings contribute to the understanding of prostate tissue remodeling after castration, as well as highlighting the rapid alterations in the prostate microenvironment in a short period following castration.

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来源期刊
CiteScore
6.00
自引率
0.00%
发文量
32
审稿时长
1 months
期刊介绍: Journal of Histochemistry & Cytochemistry (JHC) has been a pre-eminent cell biology journal for over 50 years. Published monthly, JHC offers primary research articles, timely reviews, editorials, and perspectives on the structure and function of cells, tissues, and organs, as well as mechanisms of development, differentiation, and disease. JHC also publishes new developments in microscopy and imaging, especially where imaging techniques complement current genetic, molecular and biochemical investigations of cell and tissue function. JHC offers generous space for articles and recognizing the value of images that reveal molecular, cellular and tissue organization, offers free color to all authors.
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