{"title":"基于钯修饰CoS2空心纳米盒和金功能化Fe3O4的信号放大电化学免疫传感器用于神经元特异性烯醇化酶检测","authors":"Dehao Jia, Yujian Guo, Lei Yang, Xing Gao, Dongquan Leng, Qin Wei","doi":"10.1007/s00604-025-07522-1","DOIUrl":null,"url":null,"abstract":"<div><p>A sandwich-type electrochemical (EC) immunosensor for neuron-specific enolase (NSE) detection was constructed utilizing a signal amplification strategy. This strategy was implemented by utilizing palladium decorated CoS<sub>2</sub> hollow nanoboxes (CoS<sub>2</sub>-Pd HNBs) as the signal indicator and gold-functionalized Fe<sub>3</sub>O<sub>4</sub> (Fe<sub>3</sub>O<sub>4</sub>-Au) as the EC sensing platform. CoS<sub>2</sub> HNBs with hollow structures were synthesized via template method, which exhibited excellent electrocatalytic performance toward H<sub>2</sub>O<sub>2</sub>. Moreover, Pd nanoparticles (NPs) were decorated on CoS<sub>2</sub> HNBs by in situ reduction method, which further improved the electrocatalytic performance for H<sub>2</sub>O<sub>2</sub> reduction due to the synergetic catalysis. Modifying Au NPs uniformly on Fe<sub>3</sub>O<sub>4</sub> surface could not only enhance the electroconductivity of the EC sensing platform but also immobilize the capture antibody (Ab<sub>1</sub>) via strong coordination. Fe<sub>3</sub>O<sub>4</sub>-Au nanocomposites were anchored to the magnetic glass carbon electrode because of good magnetic properties, improving stability of the EC immunosensor. Prominently, the well-established sandwich-type EC immunosensor with NSE as a targeted detection object exhibited a low detection limit of 0.29 pg/mL (S/N = 3) and a linear range from 500 fg/mL to 50 ng/mL. This work may provide a promising detection method for other biomarkers.</p><h3>Graphical abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A signal-amplifying electrochemical immunosensor based on palladium decorated CoS2 hollow nanoboxes and gold-functionalized Fe3O4 for neuron-specific enolase detection\",\"authors\":\"Dehao Jia, Yujian Guo, Lei Yang, Xing Gao, Dongquan Leng, Qin Wei\",\"doi\":\"10.1007/s00604-025-07522-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A sandwich-type electrochemical (EC) immunosensor for neuron-specific enolase (NSE) detection was constructed utilizing a signal amplification strategy. This strategy was implemented by utilizing palladium decorated CoS<sub>2</sub> hollow nanoboxes (CoS<sub>2</sub>-Pd HNBs) as the signal indicator and gold-functionalized Fe<sub>3</sub>O<sub>4</sub> (Fe<sub>3</sub>O<sub>4</sub>-Au) as the EC sensing platform. CoS<sub>2</sub> HNBs with hollow structures were synthesized via template method, which exhibited excellent electrocatalytic performance toward H<sub>2</sub>O<sub>2</sub>. Moreover, Pd nanoparticles (NPs) were decorated on CoS<sub>2</sub> HNBs by in situ reduction method, which further improved the electrocatalytic performance for H<sub>2</sub>O<sub>2</sub> reduction due to the synergetic catalysis. Modifying Au NPs uniformly on Fe<sub>3</sub>O<sub>4</sub> surface could not only enhance the electroconductivity of the EC sensing platform but also immobilize the capture antibody (Ab<sub>1</sub>) via strong coordination. Fe<sub>3</sub>O<sub>4</sub>-Au nanocomposites were anchored to the magnetic glass carbon electrode because of good magnetic properties, improving stability of the EC immunosensor. Prominently, the well-established sandwich-type EC immunosensor with NSE as a targeted detection object exhibited a low detection limit of 0.29 pg/mL (S/N = 3) and a linear range from 500 fg/mL to 50 ng/mL. This work may provide a promising detection method for other biomarkers.</p><h3>Graphical abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":705,\"journal\":{\"name\":\"Microchimica Acta\",\"volume\":\"192 10\",\"pages\":\"\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2025-09-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microchimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00604-025-07522-1\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-07522-1","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
A signal-amplifying electrochemical immunosensor based on palladium decorated CoS2 hollow nanoboxes and gold-functionalized Fe3O4 for neuron-specific enolase detection
A sandwich-type electrochemical (EC) immunosensor for neuron-specific enolase (NSE) detection was constructed utilizing a signal amplification strategy. This strategy was implemented by utilizing palladium decorated CoS2 hollow nanoboxes (CoS2-Pd HNBs) as the signal indicator and gold-functionalized Fe3O4 (Fe3O4-Au) as the EC sensing platform. CoS2 HNBs with hollow structures were synthesized via template method, which exhibited excellent electrocatalytic performance toward H2O2. Moreover, Pd nanoparticles (NPs) were decorated on CoS2 HNBs by in situ reduction method, which further improved the electrocatalytic performance for H2O2 reduction due to the synergetic catalysis. Modifying Au NPs uniformly on Fe3O4 surface could not only enhance the electroconductivity of the EC sensing platform but also immobilize the capture antibody (Ab1) via strong coordination. Fe3O4-Au nanocomposites were anchored to the magnetic glass carbon electrode because of good magnetic properties, improving stability of the EC immunosensor. Prominently, the well-established sandwich-type EC immunosensor with NSE as a targeted detection object exhibited a low detection limit of 0.29 pg/mL (S/N = 3) and a linear range from 500 fg/mL to 50 ng/mL. This work may provide a promising detection method for other biomarkers.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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