Ana Paula Remor Sebolt, Felipe Rieth de Lima, Francielli Maria Wilhelms, Ana Carolina Cavallieri, Eduardo Macagnan, Gabriela Cristini de Souza, Luiz Daniel de Barros, Pedro Volkmer de Castilho, Andreas Lazaros Chryssafidis, Anderson Barbosa de Moura
{"title":"刚地弓形虫自然感染自由生活的弗朗西斯卡纳海豚（Pontoporia blainvillei）。","authors":"Ana Paula Remor Sebolt, Felipe Rieth de Lima, Francielli Maria Wilhelms, Ana Carolina Cavallieri, Eduardo Macagnan, Gabriela Cristini de Souza, Luiz Daniel de Barros, Pedro Volkmer de Castilho, Andreas Lazaros Chryssafidis, Anderson Barbosa de Moura","doi":"10.1016/j.vprsr.2025.101321","DOIUrl":null,"url":null,"abstract":"<p><p>Toxoplasma gondii is a globally distributed protozoan capable of infecting marine animals. This study reports the first isolation of T. gondii in Pontoporia blainvillei (Franciscana dolphin), found post-mortem in Laguna, southern coast of Santa Catarina, Brazil. Necropsy was performed on a juvenile female, and pooled tissue samples (heart, lungs, brain, liver) were digested and bioassayed in mice. All three mice died within 21 days post-inoculation. Serological testing by indirect immunofluorescence assay (IFA) detected antibodies against T. gondii in two mice, with titers of 1:64 and 1:256. Tachyzoites were observed in peritoneal washes and lung imprints, and parasitic cysts were detected in brain tissue. PCR-RFLP analysis identified the ToxoDB genotype #170. This is the first report of genotype #170 in cetaceans, previously found only in alpacas, goats, and free-range chickens in North America. These results emphasize the wide dispersal potential of T. gondii and support the use of cetaceans as sentinels for marine environmental contamination.</p>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"64 ","pages":"101321"},"PeriodicalIF":1.4000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"First report of Toxoplasma gondii naturally infecting free-living Franciscana Dolphin (Pontoporia blainvillei).\",\"authors\":\"Ana Paula Remor Sebolt, Felipe Rieth de Lima, Francielli Maria Wilhelms, Ana Carolina Cavallieri, Eduardo Macagnan, Gabriela Cristini de Souza, Luiz Daniel de Barros, Pedro Volkmer de Castilho, Andreas Lazaros Chryssafidis, Anderson Barbosa de Moura\",\"doi\":\"10.1016/j.vprsr.2025.101321\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Toxoplasma gondii is a globally distributed protozoan capable of infecting marine animals. This study reports the first isolation of T. gondii in Pontoporia blainvillei (Franciscana dolphin), found post-mortem in Laguna, southern coast of Santa Catarina, Brazil. Necropsy was performed on a juvenile female, and pooled tissue samples (heart, lungs, brain, liver) were digested and bioassayed in mice. All three mice died within 21 days post-inoculation. Serological testing by indirect immunofluorescence assay (IFA) detected antibodies against T. gondii in two mice, with titers of 1:64 and 1:256. Tachyzoites were observed in peritoneal washes and lung imprints, and parasitic cysts were detected in brain tissue. PCR-RFLP analysis identified the ToxoDB genotype #170. This is the first report of genotype #170 in cetaceans, previously found only in alpacas, goats, and free-range chickens in North America. These results emphasize the wide dispersal potential of T. gondii and support the use of cetaceans as sentinels for marine environmental contamination.</p>\",\"PeriodicalId\":23600,\"journal\":{\"name\":\"Veterinary parasitology, regional studies and reports\",\"volume\":\"64 \",\"pages\":\"101321\"},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2025-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary parasitology, regional studies and reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1016/j.vprsr.2025.101321\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/8/5 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary parasitology, regional studies and reports","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.vprsr.2025.101321","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/5 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"PARASITOLOGY","Score":null,"Total":0}
First report of Toxoplasma gondii naturally infecting free-living Franciscana Dolphin (Pontoporia blainvillei).
Toxoplasma gondii is a globally distributed protozoan capable of infecting marine animals. This study reports the first isolation of T. gondii in Pontoporia blainvillei (Franciscana dolphin), found post-mortem in Laguna, southern coast of Santa Catarina, Brazil. Necropsy was performed on a juvenile female, and pooled tissue samples (heart, lungs, brain, liver) were digested and bioassayed in mice. All three mice died within 21 days post-inoculation. Serological testing by indirect immunofluorescence assay (IFA) detected antibodies against T. gondii in two mice, with titers of 1:64 and 1:256. Tachyzoites were observed in peritoneal washes and lung imprints, and parasitic cysts were detected in brain tissue. PCR-RFLP analysis identified the ToxoDB genotype #170. This is the first report of genotype #170 in cetaceans, previously found only in alpacas, goats, and free-range chickens in North America. These results emphasize the wide dispersal potential of T. gondii and support the use of cetaceans as sentinels for marine environmental contamination.
期刊介绍：
Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).
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