肥胖患者腹腔镜袖式胃切除术后GST-M、GST-P和TP53基因表达的免疫组化研究

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-09-16 DOI:10.1016/j.tice.2025.103143

Onur Di̇ri̇can , Pınar Kaygin , Burcu Kaplan , Sezen Yilmaz Sarialtin , Gülçin Güler Şi̇mşek , Serpil Oğuztüzün , Hakan Buluş , Serap Yesi̇lki̇r Baydar , Abbas Ali Husseini , Selim Öğüt

{"title":"肥胖患者腹腔镜袖式胃切除术后GST-M、GST-P和TP53基因表达的免疫组化研究","authors":"Onur Di̇ri̇can , Pınar Kaygin , Burcu Kaplan , Sezen Yilmaz Sarialtin , Gülçin Güler Şi̇mşek , Serpil Oğuztüzün , Hakan Buluş , Serap Yesi̇lki̇r Baydar , Abbas Ali Husseini , Selim Öğüt","doi":"10.1016/j.tice.2025.103143","DOIUrl":null,"url":null,"abstract":"<div><div>Obesity is linked to increased oxidative stress, contributing to various comorbidities. Glutathione S-transferases (GSTs) enzymes and tumor suppressor protein 53 (TP53) are key regulators of oxidative stress. This study aimed to examine the expression of GST-M, GST-P, and TP53 in sleeve gastrectomy tissues of obese patients. In this retrospective study, 126 obese patients who underwent sleeve gastrectomy were analyzed. Immunohistochemical staining assessed the expression of GST-M, GST-P, and TP53 in sleeve gastrectomy tissue samples. Correlation between protein expression and association with demographic, clinical, and serological data was examined. GST-P and GST-M exhibited low expression (absent/weak), while TP53 showed significantly higher expression in the sleeve gastrectomy tissue of patients with obesity. A positive and significant correlation was identified between GST-M and TP53 staining levels, while GST-P expression level did not display any correlation with GST-M and TP53. Distinct patterns of protein expression for GST-P, GST-M, and TP53 in the sleeve gastrectomy tissues of obesity patients. TP53 plays a key role in oxidative stress regulation in obesity, whereas GST-M and GST-P appear less effective.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"98 ","pages":"Article 103143"},"PeriodicalIF":2.5000,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Immunohistochemical investigation of GST-M, GST-P, and TP53 gene expression in obesity patients under laparoscopic sleeve gastrectomy\",\"authors\":\"Onur Di̇ri̇can , Pınar Kaygin , Burcu Kaplan , Sezen Yilmaz Sarialtin , Gülçin Güler Şi̇mşek , Serpil Oğuztüzün , Hakan Buluş , Serap Yesi̇lki̇r Baydar , Abbas Ali Husseini , Selim Öğüt\",\"doi\":\"10.1016/j.tice.2025.103143\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Obesity is linked to increased oxidative stress, contributing to various comorbidities. Glutathione S-transferases (GSTs) enzymes and tumor suppressor protein 53 (TP53) are key regulators of oxidative stress. This study aimed to examine the expression of GST-M, GST-P, and TP53 in sleeve gastrectomy tissues of obese patients. In this retrospective study, 126 obese patients who underwent sleeve gastrectomy were analyzed. Immunohistochemical staining assessed the expression of GST-M, GST-P, and TP53 in sleeve gastrectomy tissue samples. Correlation between protein expression and association with demographic, clinical, and serological data was examined. GST-P and GST-M exhibited low expression (absent/weak), while TP53 showed significantly higher expression in the sleeve gastrectomy tissue of patients with obesity. A positive and significant correlation was identified between GST-M and TP53 staining levels, while GST-P expression level did not display any correlation with GST-M and TP53. Distinct patterns of protein expression for GST-P, GST-M, and TP53 in the sleeve gastrectomy tissues of obesity patients. TP53 plays a key role in oxidative stress regulation in obesity, whereas GST-M and GST-P appear less effective.</div></div>\",\"PeriodicalId\":23201,\"journal\":{\"name\":\"Tissue & cell\",\"volume\":\"98 \",\"pages\":\"Article 103143\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2025-09-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tissue & cell\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0040816625004252\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ANATOMY & MORPHOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tissue & cell","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0040816625004252","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

肥胖与氧化应激增加有关，导致各种合并症。谷胱甘肽s -转移酶（GSTs）和肿瘤抑制蛋白53 （TP53）是氧化应激的关键调节因子。本研究旨在检测GST-M、GST-P和TP53在肥胖患者袖胃切除术组织中的表达。在这项回顾性研究中，我们分析了126例接受袖式胃切除术的肥胖患者。免疫组织化学染色评估GST-M、GST-P和TP53在袖胃切除术组织样本中的表达。研究了蛋白表达与人口学、临床和血清学数据的相关性。GST-P和GST-M低表达（缺失/弱），而TP53在肥胖患者的袖胃切除术组织中表达明显升高。GST-M与TP53染色水平呈显著正相关，而GST-P表达水平与GST-M和TP53无相关性。GST-P、GST-M和TP53蛋白在肥胖患者袖胃切除术组织中的不同表达模式TP53在肥胖的氧化应激调节中起着关键作用，而GST-M和GST-P似乎作用较小。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Immunohistochemical investigation of GST-M, GST-P, and TP53 gene expression in obesity patients under laparoscopic sleeve gastrectomy

Obesity is linked to increased oxidative stress, contributing to various comorbidities. Glutathione S-transferases (GSTs) enzymes and tumor suppressor protein 53 (TP53) are key regulators of oxidative stress. This study aimed to examine the expression of GST-M, GST-P, and TP53 in sleeve gastrectomy tissues of obese patients. In this retrospective study, 126 obese patients who underwent sleeve gastrectomy were analyzed. Immunohistochemical staining assessed the expression of GST-M, GST-P, and TP53 in sleeve gastrectomy tissue samples. Correlation between protein expression and association with demographic, clinical, and serological data was examined. GST-P and GST-M exhibited low expression (absent/weak), while TP53 showed significantly higher expression in the sleeve gastrectomy tissue of patients with obesity. A positive and significant correlation was identified between GST-M and TP53 staining levels, while GST-P expression level did not display any correlation with GST-M and TP53. Distinct patterns of protein expression for GST-P, GST-M, and TP53 in the sleeve gastrectomy tissues of obesity patients. TP53 plays a key role in oxidative stress regulation in obesity, whereas GST-M and GST-P appear less effective.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.