从日本陆生温泉中分离的嗜热硫酸盐还原菌的固氮能力。

IF 2 4区环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Microbes and Environments Pub Date : 2025-01-01 DOI:10.1264/jsme2.ME25030

Toko Hisano, Arisa Nishihara, Shin Haruta

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引用次数: 0

摘要

固氮硫酸盐还原细菌尚未在热环境中进行过研究。在本研究中，利用无nh3无机培养基成功地从地热泉中分离出热脱硫弧菌属菌株TK110。菌株TK110携带Calvin-Benson-Bassham循环相关基因和氮固定相关基因nifHDKENXIIB。利用乙炔还原法评估了氮酶活性，并在自养条件下检测了菌株TK110，以及在65°C异养条件下检测了Thermodesulfovibrio yellowstonii DSM 11347T。据我们所知，这是第一个证明嗜热硫酸盐还原细菌固氮的研究。

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Nitrogen-fixing Ability of a Thermophilic Sulfate-reducing Bacterium in the Genus Thermodesulfovibrio Isolated from a Terrestrial Hot Spring in Japan.

Nitrogen-fixing sulfate-reducing bacteria have not yet been exami-ned in thermal environments. In this study, strain TK110, belonging to the genus Thermodesulfovibrio, was successfully isolated from a geothermal spring using an NH₃-free inorganic medium. Strain TK110 harbored genes associated with the Calvin-Benson-Bassham cycle and nitrogen fixation-related genes, nifHDKENXIIB. Nitrogenase activity was assessed using an acetylene reduction assay and detected in strain TK110 under autotrophic conditions, as well as in Thermodesulfovibrio yellowstonii DSM 11347^T under heterotrophic conditions at 65°C. To the best of our knowledge, this is the first study to demonstrate nitrogen fixation by thermophilic sulfate-reducing bacteria.

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来源期刊

Microbes and Environments 生物-生物工程与应用微生物

CiteScore

4.10

自引率

13.60%

发文量

审稿时长

3 months

期刊介绍： Microbial ecology in natural and engineered environments; Microbial degradation of xenobiotic compounds; Microbial processes in biogeochemical cycles; Microbial interactions and signaling with animals and plants; Interactions among microorganisms; Microorganisms related to public health; Phylogenetic and functional diversity of microbial communities; Genomics, metagenomics, and bioinformatics for microbiology; Application of microorganisms to agriculture, fishery, and industry; Molecular biology and biochemistry related to environmental microbiology; Methodology in general and environmental microbiology; Interdisciplinary research areas for microbial ecology (e.g., Astrobiology, and Origins of Life); Taxonomic description of novel microorganisms with ecological perspective; Physiology and metabolisms of microorganisms; Evolution of genes and microorganisms; Genome report of microorganisms with ecological perspective.