子宫内膜癌患者血清外泌体中环状RNA 0002577的增强通过一般转录因子i - i - i重复结构域1 (GTF2IRD1)加速疾病进展。

IF 3.7 2区 医学 Q1 OBSTETRICS & GYNECOLOGY
Yueying Li, Ya Liu, Jialu Feng, Juan Du, Wenyan Tian, Liping Zhang
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引用次数: 0

摘要

目的:子宫体子宫内膜癌(UCEC)是一种常见的晚期妇科恶性肿瘤,预后较差。环状RNA (circRNA)和外泌体已被证明是肿瘤细胞进展的重要贡献者,但它们之间的具体调节机制尚不清楚。因此,我们的研究试图探讨两者之间的机制。方法:首先分离鉴定外泌体,然后通过体内和体外实验验证其在UCEC进展中的作用。其次,利用人竞争内源性RNA (human competing endogenous RNA, ceRNA)阵列从UCEC患者血清中鉴定出表达差异最显著的circRNA,并通过体外实验验证其在UCEC进展中的作用。然后,我们通过RNA测序找到该circRNA的靶基因,并通过体外实验进一步阐明两者之间的相关性及其在肿瘤细胞进展中的作用。结果:UCEC患者血清外泌体可促进UCEC的进展。人类ceRNA阵列鉴定出circRNA 0002577 (circ_0002577)上调,是改变最显著的circRNA。此外,来自UCEC患者的外泌体中circ_0002577的上调促进了UCEC的增殖和迁移。根据RNA测序结果,鉴定出一般转录因子i - i - i重复结构域1 (GTF2IRD1)基因与circ_0002577高度相关。此外,体外实验证实circ_0002577与GTF2IRD1呈正相关。结论:外泌体通过circ_0002577介导的GTF2IRD1调控促进UCEC的进展,突出了UCEC治疗的潜在治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enhancement of circular RNA 0002577 in serum exosomes in patients with endometrial cancer accelerates disease progression via general transcription factor II-I repeat domain-containing 1 (GTF2IRD1).

Objective: Uterine corpus endometrial carcinoma (UCEC) is a common gynecologic malignancy with poor prognosis in advanced stages. Circular RNA (circRNA) and exosomes have been documented as significant contributors to the advancement of tumor cells, but the specific regulatory mechanisms between them is unclear. Therefore, our study attempts to explore the mechanism between them.

Methods: Firstly, we isolated and identified exosomes, and then validated their role in UCEC progression by experiments in vivo and in vitro. Secondly, a human competing endogenous RNA (ceRNA) array was used to identify the circRNA with the most significant differences in expression from serum of UCEC patient, and validated its role in UCEC progression by experiments in vitro. Then, we find the target gene of this circRNA by RNA sequencing, and further clarify the correlation between the 2 and their role in tumor cell progression through experiments in vitro.

Results: Serum exosomes in patients with UCEC can promote the progression of UCEC. The human ceRNA array identified that circRNA 0002577 (circ_0002577) was up-regulated and was the most significantly altered circRNA. Moreover, the up-regulated circ_0002577 in exosomes derived from UCEC patients promote proliferation and migration of UCEC. Based on RNA sequencing results, general transcription factor II-I repeat domain-containing 1 (GTF2IRD1) gene was identified as being highly correlated with circ_0002577. Additionally, a positive correlation between circ_0002577 and GTF2IRD1 was confirmed by experiments in vitro.

Conclusion: Exosomes promote UCEC progression through circ_0002577 mediated regulation of GTF2IRD1, highlighting the potential therapeutic targets in treatment for UCEC.

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来源期刊
Journal of Gynecologic Oncology
Journal of Gynecologic Oncology ONCOLOGY-OBSTETRICS & GYNECOLOGY
CiteScore
6.00
自引率
2.60%
发文量
84
审稿时长
>12 weeks
期刊介绍: The Journal of Gynecologic Oncology (JGO) is an official publication of the Asian Society of Gynecologic Oncology. Abbreviated title is ''J Gynecol Oncol''. It was launched in 1990. The JGO''s aim is to publish the highest quality manuscripts dedicated to the advancement of care of the patients with gynecologic cancer. It is an international peer-reviewed periodical journal that is published bimonthly (January, March, May, July, September, and November). Supplement numbers are at times published. The journal publishes editorials, original and review articles, correspondence, book review, etc.
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