Jae Kyeong Lee, Min Woo Lee, Chae Yeong Moon, Jeong Min Kim, Hülya Bayburt, Byeong Jun Choi, Che Ok Jeon
{"title":"产类胡萝卜素的齐彭元藻和齐彭元藻的分离及齐彭元藻属的修订描述，徐等。2020。","authors":"Jae Kyeong Lee, Min Woo Lee, Chae Yeong Moon, Jeong Min Kim, Hülya Bayburt, Byeong Jun Choi, Che Ok Jeon","doi":"10.4014/jmb.2507.07023","DOIUrl":null,"url":null,"abstract":"<p><p>Two Gram-stain-negative, facultatively aerobic, non-motile, catalase- and oxidase-positive, rod-shaped bacteria, designated strains DGS2-2<sup>T</sup> (orange-pigmented) and DGS5-3<sup>T</sup> (yellow-pigmented), were isolated from marine red algae collected in Korea. Strain DGS2-2<sup>T</sup> grew at 20-40°C, pH 6.0-10.0, and in 1.0-6.0% (w/v) NaCl, while DGS5-3<sup>T</sup> grew at 15-40°C, pH 6.0-10.0, and in 2.0-6.0% NaCl. Ubiquinone-10 was the sole respiratory quinone. The G+C contents were 62.5% for DGS2-2<sup>T</sup> and 57.5% for DGS5-3<sup>T</sup>. Both strains contained summed feature 8 (C<sub>18:1</sub><i>ω</i>7<i>c</i> and/or C<sub>18:1</sub><i>ω</i>6<i>c</i>) and C<sub>16:0</sub> as major fatty acids, and phosphatidylcholine, sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol as major polar lipids. The 16S rRNA gene similarity (97.3%), average nucleotide identity (ANI, 72.0%), and digital DNA-DNA hybridization (dDDH, 18.4%) values between the two strains were below the species delineation thresholds. Phylogenetic and phylogenomic analyses based on 16S rRNA gene and whole-genome sequences placed both strains in distinct lineages within the genus <i>Qipengyuania</i>. ANI and dDDH values between each strain and <i>Qipengyuania</i> type strains were below 74.5% and 19.5%, respectively, supporting their designation as novel species. Genomic analyses identified putative genes associated with potential algal symbiotic traits, including the biosynthesis of vitamins, siderophores, and hormone-like compounds. Carotenoid biosynthetic genes were also identified, and LC/MS confirmed astaxanthin (DGS2-2<sup>T</sup>) and nostoxanthin (DGS5-3<sup>T</sup>) production. Based on genomic, phylogenetic, phenotypic, and chemotaxonomic evidence, strains DGS2-2<sup>T</sup> and DGS5-3<sup>T</sup> represent two novel species of <i>Qipengyuania</i>, for which the names <i>Qipengyuania algicola</i> sp. nov. (DGS2-2<sup>T</sup> =KACC 23855<sup>T</sup> =JCM 37496<sup>T</sup>) and <i>Qipengyuania rhodophyticola</i> sp. nov. (DGS5-3<sup>T</sup> =KACC 23854<sup>T</sup> =JCM 37497<sup>T</sup>) are proposed.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2507023"},"PeriodicalIF":3.1000,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476985/pdf/","citationCount":"0","resultStr":"{\"title\":\"Carotenoid-Producing <i>Qipengyuania algicola</i> sp. nov. and <i>Qipengyuania rhodophyticola</i> sp. nov., Isolated from Marine Algae, and Emended Description of the Genus <i>Qipengyuania</i> Xu <i>et al</i>. 2020.\",\"authors\":\"Jae Kyeong Lee, Min Woo Lee, Chae Yeong Moon, Jeong Min Kim, Hülya Bayburt, Byeong Jun Choi, Che Ok Jeon\",\"doi\":\"10.4014/jmb.2507.07023\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Two Gram-stain-negative, facultatively aerobic, non-motile, catalase- and oxidase-positive, rod-shaped bacteria, designated strains DGS2-2<sup>T</sup> (orange-pigmented) and DGS5-3<sup>T</sup> (yellow-pigmented), were isolated from marine red algae collected in Korea. Strain DGS2-2<sup>T</sup> grew at 20-40°C, pH 6.0-10.0, and in 1.0-6.0% (w/v) NaCl, while DGS5-3<sup>T</sup> grew at 15-40°C, pH 6.0-10.0, and in 2.0-6.0% NaCl. Ubiquinone-10 was the sole respiratory quinone. The G+C contents were 62.5% for DGS2-2<sup>T</sup> and 57.5% for DGS5-3<sup>T</sup>. Both strains contained summed feature 8 (C<sub>18:1</sub><i>ω</i>7<i>c</i> and/or C<sub>18:1</sub><i>ω</i>6<i>c</i>) and C<sub>16:0</sub> as major fatty acids, and phosphatidylcholine, sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol as major polar lipids. The 16S rRNA gene similarity (97.3%), average nucleotide identity (ANI, 72.0%), and digital DNA-DNA hybridization (dDDH, 18.4%) values between the two strains were below the species delineation thresholds. Phylogenetic and phylogenomic analyses based on 16S rRNA gene and whole-genome sequences placed both strains in distinct lineages within the genus <i>Qipengyuania</i>. ANI and dDDH values between each strain and <i>Qipengyuania</i> type strains were below 74.5% and 19.5%, respectively, supporting their designation as novel species. Genomic analyses identified putative genes associated with potential algal symbiotic traits, including the biosynthesis of vitamins, siderophores, and hormone-like compounds. Carotenoid biosynthetic genes were also identified, and LC/MS confirmed astaxanthin (DGS2-2<sup>T</sup>) and nostoxanthin (DGS5-3<sup>T</sup>) production. Based on genomic, phylogenetic, phenotypic, and chemotaxonomic evidence, strains DGS2-2<sup>T</sup> and DGS5-3<sup>T</sup> represent two novel species of <i>Qipengyuania</i>, for which the names <i>Qipengyuania algicola</i> sp. nov. (DGS2-2<sup>T</sup> =KACC 23855<sup>T</sup> =JCM 37496<sup>T</sup>) and <i>Qipengyuania rhodophyticola</i> sp. nov. (DGS5-3<sup>T</sup> =KACC 23854<sup>T</sup> =JCM 37497<sup>T</sup>) are proposed.</p>\",\"PeriodicalId\":16481,\"journal\":{\"name\":\"Journal of microbiology and biotechnology\",\"volume\":\"35 \",\"pages\":\"e2507023\"},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2025-09-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476985/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of microbiology and biotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.4014/jmb.2507.07023\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of microbiology and biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.4014/jmb.2507.07023","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Carotenoid-Producing Qipengyuania algicola sp. nov. and Qipengyuania rhodophyticola sp. nov., Isolated from Marine Algae, and Emended Description of the Genus Qipengyuania Xu et al. 2020.
Two Gram-stain-negative, facultatively aerobic, non-motile, catalase- and oxidase-positive, rod-shaped bacteria, designated strains DGS2-2T (orange-pigmented) and DGS5-3T (yellow-pigmented), were isolated from marine red algae collected in Korea. Strain DGS2-2T grew at 20-40°C, pH 6.0-10.0, and in 1.0-6.0% (w/v) NaCl, while DGS5-3T grew at 15-40°C, pH 6.0-10.0, and in 2.0-6.0% NaCl. Ubiquinone-10 was the sole respiratory quinone. The G+C contents were 62.5% for DGS2-2T and 57.5% for DGS5-3T. Both strains contained summed feature 8 (C18:1ω7c and/or C18:1ω6c) and C16:0 as major fatty acids, and phosphatidylcholine, sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol as major polar lipids. The 16S rRNA gene similarity (97.3%), average nucleotide identity (ANI, 72.0%), and digital DNA-DNA hybridization (dDDH, 18.4%) values between the two strains were below the species delineation thresholds. Phylogenetic and phylogenomic analyses based on 16S rRNA gene and whole-genome sequences placed both strains in distinct lineages within the genus Qipengyuania. ANI and dDDH values between each strain and Qipengyuania type strains were below 74.5% and 19.5%, respectively, supporting their designation as novel species. Genomic analyses identified putative genes associated with potential algal symbiotic traits, including the biosynthesis of vitamins, siderophores, and hormone-like compounds. Carotenoid biosynthetic genes were also identified, and LC/MS confirmed astaxanthin (DGS2-2T) and nostoxanthin (DGS5-3T) production. Based on genomic, phylogenetic, phenotypic, and chemotaxonomic evidence, strains DGS2-2T and DGS5-3T represent two novel species of Qipengyuania, for which the names Qipengyuania algicola sp. nov. (DGS2-2T =KACC 23855T =JCM 37496T) and Qipengyuania rhodophyticola sp. nov. (DGS5-3T =KACC 23854T =JCM 37497T) are proposed.
期刊介绍：
The Journal of Microbiology and Biotechnology (JMB) is a monthly international journal devoted to the advancement and dissemination of scientific knowledge pertaining to microbiology, biotechnology, and related academic disciplines. It covers various scientific and technological aspects of Molecular and Cellular Microbiology, Environmental Microbiology and Biotechnology, Food Biotechnology, and Biotechnology and Bioengineering (subcategories are listed below). Launched in March 1991, the JMB is published by the Korean Society for Microbiology and Biotechnology (KMB) and distributed worldwide.
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