原发性纤毛运动障碍患者痰粘弹性和气道炎症的变化与elexaftor /Tezacaftor/Ivacaftor治疗后的囊性纤维化相当。

IF 21 1区 医学 Q1 RESPIRATORY SYSTEM
Hannah Nussstein,Ruth M Urbantat,Kerstin Fentker,Aditi Loewe,Julia Duerr,Mohamed Haji,Felix Doellinger,Mirjam Stahl,Simon Y Graeber,Michael Gradzielski,Jobst Röhmel,Philipp Mertins,Laura Schaupp,Marcus A Mall
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引用次数: 0

摘要

背景:原发性纤毛运动障碍(PCD)和囊性纤维化(CF)是由独特的遗传决定的纤毛清除缺陷引起的粘膜阻塞性肺疾病,然而,关于气道粘液功能障碍和慢性炎症的相对严重程度的知识仍然有限。因此,本研究的目的是比较PCD患者和CF患者在使用ETI治疗前后的痰黏弹性、炎症标志物和蛋白质组学。方法比较42例临床稳定的青少年和成人PCD患者、40例至少有一个F508del等位基因的CF患者和15例年龄匹配的健康对照者的痰流变学、炎症标志物和蛋白质组学。结果PCD患者痰液弹性模量(G′)和黏性模量(G″)均高于健康对照组(p<0.001),低于CF组基线值(p<0.001),与ETI组相似。PCD患者痰中的炎症标志物包括中性粒细胞弹性酶(NE)、游离DNA、髓过氧化物酶(MPO)、白细胞介素(IL)-1β和IL-8也比健康对照组升高(均p<0.001),低于基线时的CF (p<0.05至p<0.001),与ETI时的CF相当。同样,与基线时的CF相比,PCD患者的痰蛋白组变化不太明显,但在ETI上PCD和CF之间具有可比性。结论临床稳定的PCD患者的痰黏弹性、炎症标志物和蛋白质组的变化在基线时比CF患者轻,但与接受ETI治疗的CF患者相当。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Changes in Sputum Viscoelastic Properties and Airway Inflammation in Primary Ciliary Dyskinesia are Comparable to Cystic Fibrosis on Elexacaftor/Tezacaftor/Ivacaftor Therapy.
BACKGROUND Primary ciliary dyskinesia (PCD) and cystic fibrosis (CF) are muco-obstructive lung diseases that are caused by distinct genetically determined defects in mucociliary clearance, however, knowledge on the relative severity of airway mucus dysfunction and chronic inflammation remains limited. The aim of this study was therefore to compare sputum viscoelastic properties, inflammation markers and the proteome between patients with PCD and patients with CF before and under elexacaftor/tezacaftor/ivacaftor (ETI) therapy. METHODS We compared sputum rheology, inflammation markers and the proteome in 42 clinically stable adolescent and adult patients with PCD, 40 patients with CF with at least one F508del allele before (baseline) and 3 months after initiation of ETI and 15 age-matched healthy controls. RESULTS The elastic modulus (G') and viscous modulus (G″) of PCD sputum was increased compared to healthy controls (p<0.001), lower than in CF at baseline (p<0.001) and similar to CF on ETI. Inflammation markers in PCD sputum including neutrophil elastase (NE), free DNA, myeloperoxidase (MPO), interleukin (IL)-1β and IL-8 were also increased compared to healthy controls (all p<0.001), lower than in CF at baseline (p<0.05 to p<0.001) and comparable to CF on ETI. Similar, changes in the sputum proteome were less pronounced in PCD compared to CF at baseline, but comparable between PCD and CF on ETI. CONCLUSIONS Clinically stable patients with PCD show changes in sputum viscoelastic properties, inflammation markers and the proteome that are less severe than in patients with CF at baseline, but comparable to CF patients on ETI therapy.
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来源期刊
European Respiratory Journal
European Respiratory Journal 医学-呼吸系统
CiteScore
27.50
自引率
3.30%
发文量
345
审稿时长
2-4 weeks
期刊介绍: The European Respiratory Journal (ERJ) is the flagship journal of the European Respiratory Society. It has a current impact factor of 24.9. The journal covers various aspects of adult and paediatric respiratory medicine, including cell biology, epidemiology, immunology, oncology, pathophysiology, imaging, occupational medicine, intensive care, sleep medicine, and thoracic surgery. In addition to original research material, the ERJ publishes editorial commentaries, reviews, short research letters, and correspondence to the editor. The articles are published continuously and collected into 12 monthly issues in two volumes per year.
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