Xinyang Yue, Shuang Liang, Haiyue Zhang, Tenglong Dai, Jun Wu
{"title":"衰老加速因子（CD55）阳性细胞外囊泡与老年人年龄和血糖水平的关系","authors":"Xinyang Yue, Shuang Liang, Haiyue Zhang, Tenglong Dai, Jun Wu","doi":"10.2147/CIA.S536479","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Extracellular vesicles (EVs) play a pivotal role in driving aging, serving as vehicles for the transmission of aging-related signals. Decay-accelerating factors (DAFs), also known as CD55, a crucial complement regulator, exhibits age-associated alterations, yet its expression on circulating EVs remains poorly characterized. This study aims to investigate differences in plasma EVs and CD55 positive EVs concentrations in younger and older adults, and explore their correlations with age and blood glucose levels.</p><p><strong>Methods: </strong>In this study, 40 older adults (≥65 years) undergoing routine physical examinations at Beijing Jishuitan Hospital and 40 gender-matched younger adults (<65 years) were enrolled prospectively. Blood glucose levels were measured using the standard glucose oxidase method. Circulating EVs concentrations were quantified by flow cytometry, including circulating total EVs, endothelial EVs (EEVs), platelet EVs (PEVs), red blood cell EVs (REVs), lymphocyte EVs (LEVs), monocyte EVs (MEVs), neutrophil EVs (NEVs), white blood cell EVs (WEVs), and CD55-positive EVs.</p><p><strong>Results: </strong>Compared to younger adults, older adults exhibited significantly lower concentrations of LEVs, MEVs, NEVs, and WEVs (<i>p</i> < 0.05). Concentrations of CD55 positive EVs, including CD55+EEVs, CD55+PEVs, CD55+LEVs, CD55+MEVs, CD55+NEVs, and CD55+WEVs, were markedly reduced in older adults (<i>p</i> < 0.05). Notably, CD55+PEVs showed the strongest negative correlation with age (r = - 0.6228, <i>p</i> < 0.001). Furthermore, significant inverse correlations were found between blood glucose levels and concentrations of CD55+EEVs (<i>p</i> = 0.001), CD55+PEVs (<i>p</i> = 0.006), CD55+LEVs (<i>p</i> < 0.001), CD55+MEVs (<i>p</i> = 0.001), and CD55+WEVs (<i>p</i> = 0.003).</p><p><strong>Conclusion: </strong>Our findings indicate significant differences in plasma EVs subtypes between older and younger adults and demonstrate robust negative correlations between CD55+ EVs subtypes and both aging and elevated glucose levels. Thus, these results suggest CD55+ EVs as a critical contributor to metabolic disorders in aging, offering potential risk assessment and monitoring strategies in older populations.</p>","PeriodicalId":48841,"journal":{"name":"Clinical Interventions in Aging","volume":"20 ","pages":"1549-1560"},"PeriodicalIF":3.7000,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12439711/pdf/","citationCount":"0","resultStr":"{\"title\":\"Association of Decay Accelerating Factor (CD55) Positive Extracellular Vesicles with Advanced Age and Blood Glucose Levels in Elderly Individuals.\",\"authors\":\"Xinyang Yue, Shuang Liang, Haiyue Zhang, Tenglong Dai, Jun Wu\",\"doi\":\"10.2147/CIA.S536479\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Extracellular vesicles (EVs) play a pivotal role in driving aging, serving as vehicles for the transmission of aging-related signals. Decay-accelerating factors (DAFs), also known as CD55, a crucial complement regulator, exhibits age-associated alterations, yet its expression on circulating EVs remains poorly characterized. This study aims to investigate differences in plasma EVs and CD55 positive EVs concentrations in younger and older adults, and explore their correlations with age and blood glucose levels.</p><p><strong>Methods: </strong>In this study, 40 older adults (≥65 years) undergoing routine physical examinations at Beijing Jishuitan Hospital and 40 gender-matched younger adults (<65 years) were enrolled prospectively. Blood glucose levels were measured using the standard glucose oxidase method. Circulating EVs concentrations were quantified by flow cytometry, including circulating total EVs, endothelial EVs (EEVs), platelet EVs (PEVs), red blood cell EVs (REVs), lymphocyte EVs (LEVs), monocyte EVs (MEVs), neutrophil EVs (NEVs), white blood cell EVs (WEVs), and CD55-positive EVs.</p><p><strong>Results: </strong>Compared to younger adults, older adults exhibited significantly lower concentrations of LEVs, MEVs, NEVs, and WEVs (<i>p</i> < 0.05). Concentrations of CD55 positive EVs, including CD55+EEVs, CD55+PEVs, CD55+LEVs, CD55+MEVs, CD55+NEVs, and CD55+WEVs, were markedly reduced in older adults (<i>p</i> < 0.05). Notably, CD55+PEVs showed the strongest negative correlation with age (r = - 0.6228, <i>p</i> < 0.001). Furthermore, significant inverse correlations were found between blood glucose levels and concentrations of CD55+EEVs (<i>p</i> = 0.001), CD55+PEVs (<i>p</i> = 0.006), CD55+LEVs (<i>p</i> < 0.001), CD55+MEVs (<i>p</i> = 0.001), and CD55+WEVs (<i>p</i> = 0.003).</p><p><strong>Conclusion: </strong>Our findings indicate significant differences in plasma EVs subtypes between older and younger adults and demonstrate robust negative correlations between CD55+ EVs subtypes and both aging and elevated glucose levels. Thus, these results suggest CD55+ EVs as a critical contributor to metabolic disorders in aging, offering potential risk assessment and monitoring strategies in older populations.</p>\",\"PeriodicalId\":48841,\"journal\":{\"name\":\"Clinical Interventions in Aging\",\"volume\":\"20 \",\"pages\":\"1549-1560\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2025-09-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12439711/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical Interventions in Aging\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.2147/CIA.S536479\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"GERIATRICS & GERONTOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical Interventions in Aging","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2147/CIA.S536479","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"GERIATRICS & GERONTOLOGY","Score":null,"Total":0}
Association of Decay Accelerating Factor (CD55) Positive Extracellular Vesicles with Advanced Age and Blood Glucose Levels in Elderly Individuals.
Background: Extracellular vesicles (EVs) play a pivotal role in driving aging, serving as vehicles for the transmission of aging-related signals. Decay-accelerating factors (DAFs), also known as CD55, a crucial complement regulator, exhibits age-associated alterations, yet its expression on circulating EVs remains poorly characterized. This study aims to investigate differences in plasma EVs and CD55 positive EVs concentrations in younger and older adults, and explore their correlations with age and blood glucose levels.
Methods: In this study, 40 older adults (≥65 years) undergoing routine physical examinations at Beijing Jishuitan Hospital and 40 gender-matched younger adults (<65 years) were enrolled prospectively. Blood glucose levels were measured using the standard glucose oxidase method. Circulating EVs concentrations were quantified by flow cytometry, including circulating total EVs, endothelial EVs (EEVs), platelet EVs (PEVs), red blood cell EVs (REVs), lymphocyte EVs (LEVs), monocyte EVs (MEVs), neutrophil EVs (NEVs), white blood cell EVs (WEVs), and CD55-positive EVs.
Results: Compared to younger adults, older adults exhibited significantly lower concentrations of LEVs, MEVs, NEVs, and WEVs (p < 0.05). Concentrations of CD55 positive EVs, including CD55+EEVs, CD55+PEVs, CD55+LEVs, CD55+MEVs, CD55+NEVs, and CD55+WEVs, were markedly reduced in older adults (p < 0.05). Notably, CD55+PEVs showed the strongest negative correlation with age (r = - 0.6228, p < 0.001). Furthermore, significant inverse correlations were found between blood glucose levels and concentrations of CD55+EEVs (p = 0.001), CD55+PEVs (p = 0.006), CD55+LEVs (p < 0.001), CD55+MEVs (p = 0.001), and CD55+WEVs (p = 0.003).
Conclusion: Our findings indicate significant differences in plasma EVs subtypes between older and younger adults and demonstrate robust negative correlations between CD55+ EVs subtypes and both aging and elevated glucose levels. Thus, these results suggest CD55+ EVs as a critical contributor to metabolic disorders in aging, offering potential risk assessment and monitoring strategies in older populations.
期刊介绍：
Clinical Interventions in Aging, is an online, peer reviewed, open access journal focusing on concise rapid reporting of original research and reviews in aging. Special attention will be given to papers reporting on actual or potential clinical applications leading to improved prevention or treatment of disease or a greater understanding of pathological processes that result from maladaptive changes in the body associated with aging. This journal is directed at a wide array of scientists, engineers, pharmacists, pharmacologists and clinical specialists wishing to maintain an up to date knowledge of this exciting and emerging field.
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