Miles F Bartlett, Andrew P Oneglia, Delaney Davis, Sauyeh Zamani, Ashfaq Siddiqui, Mark D Ricard, Michael D Nelson
{"title":"最大努力的膝关节伸展运动损害骨骼肌氧化能力和体内VO2的恢复。","authors":"Miles F Bartlett, Andrew P Oneglia, Delaney Davis, Sauyeh Zamani, Ashfaq Siddiqui, Mark D Ricard, Michael D Nelson","doi":"10.1152/japplphysiol.00517.2025","DOIUrl":null,"url":null,"abstract":"<p><p>In the present study, we examined how fatiguing exercise affects O<sub>2</sub>-based measures of skeletal muscle oxidative capacity <i>in vivo</i> by measuring changes in the rate constant of muscle VO<sub>2</sub> recovery (<i>k</i><sub>VO2</sub>). Healthy young adults completed isokinetic (120º∙s<sup>-1</sup>), maximal voluntary dynamic contractions (MVDCs) lasting 24- (baseline <i>k</i><sub>VO2</sub>) and 240 s (post-fatiguing exercise <i>k</i><sub>VO2</sub>). Vastus lateralis <i>k</i><sub>VO2</sub> was measured using near-infrared diffuse correlation spectroscopy (NIRS-DCS) via the conventional repeated arterial occlusion method (Part-A, n=14) or a novel NIRS-DCS 'free-flow' method (Part-B, n=13). Pulmonary VO<sub>2</sub> (pVO<sub>2</sub>), muscle VO<sub>2</sub> (mVO<sub>2</sub>), and surface electromyography (sEMG) measures of muscle activation were also measured throughout the 240-s trial. Compared to the 24-s trial, <i>k</i><sub>VO2</sub> following 240s of MVDCs was impaired by ~25% (Part-A; p=0.005) and ~16% (Part-B; p=0.017). Moreover, both pVO<sub>2</sub> and mVO<sub>2</sub> rapidly increased to maximal levels, where they remained for the duration of the 240-s trial, despite sEMG activity and peak MVDC power declining. These results demonstrate that fatiguing exercise not only impairs O<sub>2</sub>-based measures of skeletal muscle oxidative capacity, but that mitochondrial O<sub>2</sub>-consumption is uncoupled from power output and ATP demand during fatiguing exercise.</p>","PeriodicalId":15160,"journal":{"name":"Journal of applied physiology","volume":" ","pages":""},"PeriodicalIF":3.3000,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Maximal-effort knee-extension exercise impairs skeletal muscle oxidative capacity and VO<sub>2</sub> recovery <i>in vivo</i>.\",\"authors\":\"Miles F Bartlett, Andrew P Oneglia, Delaney Davis, Sauyeh Zamani, Ashfaq Siddiqui, Mark D Ricard, Michael D Nelson\",\"doi\":\"10.1152/japplphysiol.00517.2025\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In the present study, we examined how fatiguing exercise affects O<sub>2</sub>-based measures of skeletal muscle oxidative capacity <i>in vivo</i> by measuring changes in the rate constant of muscle VO<sub>2</sub> recovery (<i>k</i><sub>VO2</sub>). Healthy young adults completed isokinetic (120º∙s<sup>-1</sup>), maximal voluntary dynamic contractions (MVDCs) lasting 24- (baseline <i>k</i><sub>VO2</sub>) and 240 s (post-fatiguing exercise <i>k</i><sub>VO2</sub>). Vastus lateralis <i>k</i><sub>VO2</sub> was measured using near-infrared diffuse correlation spectroscopy (NIRS-DCS) via the conventional repeated arterial occlusion method (Part-A, n=14) or a novel NIRS-DCS 'free-flow' method (Part-B, n=13). Pulmonary VO<sub>2</sub> (pVO<sub>2</sub>), muscle VO<sub>2</sub> (mVO<sub>2</sub>), and surface electromyography (sEMG) measures of muscle activation were also measured throughout the 240-s trial. Compared to the 24-s trial, <i>k</i><sub>VO2</sub> following 240s of MVDCs was impaired by ~25% (Part-A; p=0.005) and ~16% (Part-B; p=0.017). Moreover, both pVO<sub>2</sub> and mVO<sub>2</sub> rapidly increased to maximal levels, where they remained for the duration of the 240-s trial, despite sEMG activity and peak MVDC power declining. These results demonstrate that fatiguing exercise not only impairs O<sub>2</sub>-based measures of skeletal muscle oxidative capacity, but that mitochondrial O<sub>2</sub>-consumption is uncoupled from power output and ATP demand during fatiguing exercise.</p>\",\"PeriodicalId\":15160,\"journal\":{\"name\":\"Journal of applied physiology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2025-09-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of applied physiology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1152/japplphysiol.00517.2025\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PHYSIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of applied physiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1152/japplphysiol.00517.2025","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PHYSIOLOGY","Score":null,"Total":0}
Maximal-effort knee-extension exercise impairs skeletal muscle oxidative capacity and VO2 recovery in vivo.
In the present study, we examined how fatiguing exercise affects O2-based measures of skeletal muscle oxidative capacity in vivo by measuring changes in the rate constant of muscle VO2 recovery (kVO2). Healthy young adults completed isokinetic (120º∙s-1), maximal voluntary dynamic contractions (MVDCs) lasting 24- (baseline kVO2) and 240 s (post-fatiguing exercise kVO2). Vastus lateralis kVO2 was measured using near-infrared diffuse correlation spectroscopy (NIRS-DCS) via the conventional repeated arterial occlusion method (Part-A, n=14) or a novel NIRS-DCS 'free-flow' method (Part-B, n=13). Pulmonary VO2 (pVO2), muscle VO2 (mVO2), and surface electromyography (sEMG) measures of muscle activation were also measured throughout the 240-s trial. Compared to the 24-s trial, kVO2 following 240s of MVDCs was impaired by ~25% (Part-A; p=0.005) and ~16% (Part-B; p=0.017). Moreover, both pVO2 and mVO2 rapidly increased to maximal levels, where they remained for the duration of the 240-s trial, despite sEMG activity and peak MVDC power declining. These results demonstrate that fatiguing exercise not only impairs O2-based measures of skeletal muscle oxidative capacity, but that mitochondrial O2-consumption is uncoupled from power output and ATP demand during fatiguing exercise.
期刊介绍:
The Journal of Applied Physiology publishes the highest quality original research and reviews that examine novel adaptive and integrative physiological mechanisms in humans and animals that advance the field. The journal encourages the submission of manuscripts that examine the acute and adaptive responses of various organs, tissues, cells and/or molecular pathways to environmental, physiological and/or pathophysiological stressors. As an applied physiology journal, topics of interest are not limited to a particular organ system. The journal, therefore, considers a wide array of integrative and translational research topics examining the mechanisms involved in disease processes and mitigation strategies, as well as the promotion of health and well-being throughout the lifespan. Priority is given to manuscripts that provide mechanistic insight deemed to exert an impact on the field.