人巨细胞病毒在egfr突变和野生型肺腺癌中的作用:细胞学涂片检测和相关的外周免疫反应

IF 1 4区 医学 Q4 MEDICAL LABORATORY TECHNOLOGY
Suzana Harabajsa, Paula Gršković, Ivana Grgić, Lana Gorenec, Snježana Židovec Lepej, Sonja Badovinac, Marko Jakopović, Silvana Smojver-Ježek, Petra Korać
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引用次数: 0

摘要

背景:人巨细胞病毒(HCMV)已被认为是肺癌的潜在危险因素。本研究旨在探讨肿瘤细胞中HCMV的存在与EGFR突变型和EGFR野生型肺腺癌患者外周血对HCMV的免疫应答之间的关系。方法:提取102例may - gr nwald- giemsa染色细胞学涂片(51例EGFR突变型和51例EGFR野生型)的DNA。采用PCR方法检测病毒基因MIE和gB在肿瘤细胞中的存在。采用QuantiFERON-CMV酶联免疫吸附试验评估80例相应外周血样本对HCMV的免疫反应。结果:EGFR突变型涂片8例(15.7%)、野生型涂片1例(2.0%)检出HCMV MIE基因。在4个(7.8%)EGFR野生型涂片中检测到HCMV gB基因,而在EGFR突变涂片中未发现HCMV gB基因。18例EGFR突变患者(62.1%)和26例EGFR野生型患者(50.9%)对HCMV有免疫应答。在检测到HCMV MIE的患者中,在两名egfr突变患者和一名egfr阴性患者中发现了免疫应答。在HCMV gB阳性涂片中,在3例EGFR野生型病例中检测到免疫应答。尽管存在HCMV gB,但1例EGFR野生型病例未显示免疫应答。结论:无论EGFR基因是否突变,肺腺癌细胞中的HCMV与外周血中对HCMV的免疫反应均无明显相关性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Human Cytomegalovirus in EGFR-Mutated and Wild-Type Lung Adenocarcinoma: Detection in Cytological Smears and Associated Peripheral Immune Response.

Background: Human cytomegalovirus (HCMV) has been proposed as a potential risk factor in lung cancer. This study aimed to investigate the relationship between the presence of HCMV in tumor cells and the immune response to HCMV in the peripheral blood of patients with EGFR-mutated and EGFR wild-type lung adenocarcinoma.

Methods: DNA was extracted from 102 May-Grünwald-Giemsa-stained cytological smears (51 EGFR-mutated and 51 EGFR wild-type). HCMV presence in tumor cells was assessed by PCR targeting viral genes MIE and gB. The immune response to HCMV was evaluated in 80 corresponding peripheral blood samples using the QuantiFERON-CMV ELISA test. Statistical analysis was performed using the chi-square test, with significance p < 0.05.

Results: HCMV MIE gene was detected in eight (15.7%) EGFR-mutated and one (2.0%) EGFR wild-type smear. The HCMV gB gene was identified in four (7.8%) EGFR wild-type smears and none of the EGFR-mutated smears. Immune response to HCMV was observed in 18 (62.1%) EGFR-mutated and 26 (50.9%) EGFR wild-type patients. Among those with detectable HCMV MIE, an immune response was found in two EGFR-mutated and one EGFR-negative patient. Among smears positive for HCMV gB, immune response was detected in three EGFR wild-type cases. One EGFR wild-type case showed no immune response despite the presence of HCMV gB.

Conclusion: No clear association was observed between HCMV in lung adenocarcinoma cells and the immune response to HCMV in peripheral blood, regardless of EGFR gene mutation status.

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来源期刊
Diagnostic Cytopathology
Diagnostic Cytopathology 医学-病理学
CiteScore
2.60
自引率
7.70%
发文量
163
审稿时长
3-6 weeks
期刊介绍: Diagnostic Cytopathology is intended to provide a forum for the exchange of information in the field of cytopathology, with special emphasis on the practical, clinical aspects of the discipline. The editors invite original scientific articles, as well as special review articles, feature articles, and letters to the editor, from laboratory professionals engaged in the practice of cytopathology. Manuscripts are accepted for publication on the basis of scientific merit, practical significance, and suitability for publication in a journal dedicated to this discipline. Original articles can be considered only with the understanding that they have never been published before and that they have not been submitted for simultaneous review to another publication.
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