槲皮素对人间充质干细胞表型特征影响的研究。

IF 5 4区 医学 Q3 BIOPHYSICS
Cellular and molecular bioengineering Pub Date : 2025-05-30 eCollection Date: 2025-08-01 DOI:10.1007/s12195-025-00849-y
Thomas Needy, David Heinrichs, Vitali Maldonado, Ryan Michael Porter, Hanna Jensen, C Lowry Barnes, Rebekah Margaret Samsonraj
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引用次数: 0

摘要

间充质干细胞(MSC)效力和治疗效用的一个重大障碍是体外衰老,这是一种与年龄相关并发症相关的不可逆转的复制停止。抗衰老药物,如槲皮素,可能有助于选择性地剔除衰老细胞,同时使非衰老细胞不受影响,从而增加高传代MSCs的效力。方法:采用体外模型评价槲皮素的表型、基因型和免疫调节作用。将MSCs体外反复传代培养的衰老细胞和非衰老细胞分别用10 μM槲皮素处理,分化为骨细胞、脂肪细胞和软骨细胞,并分析观察槲皮素的作用。结果:槲皮素对MSC功能无促进作用。SAβ-gal和活死染色表明,它没有表现出一致的抗衰老作用,在一些供体中短期内阻碍了增殖,并降低了成骨标志物COL1A1和ALP的表达。然而,槲皮素治疗对脂肪形成、软骨形成或吲哚胺2,3双加氧酶分泌没有负面影响。结论:本研究有助于揭示槲皮素的性质及其对MSC体外培养和功能的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Examining the Effects of Quercetin on Phenotypic Characteristics of Human Mesenchymal Stem Cells.

Examining the Effects of Quercetin on Phenotypic Characteristics of Human Mesenchymal Stem Cells.

Examining the Effects of Quercetin on Phenotypic Characteristics of Human Mesenchymal Stem Cells.

Examining the Effects of Quercetin on Phenotypic Characteristics of Human Mesenchymal Stem Cells.

Introduction: A significant obstacle to mesenchymal stem cell (MSC) potency and therapeutic utility is in vitro senescence, an irreversible cessation of replication associated with age-related complications. Senolytic drugs, such as quercetin, may be helpful in selectively culling senescent cells while leaving non-senescent cells unaffected, thereby increasing potency of high-passage MSCs.

Methods: The phenotypic, genotypic, and immunomodulatory effects of quercetin were assessed using in vitro models. Senescent cells, created through repeated subculturing of MSCs in vitro, and non-senescent cells were treated with 10 μM quercetin, differentiated into osteocytes, adipocytes, and chondrocytes, and analyzed to observe the effect of quercetin.

Results: Quercetin was not found to be beneficial to MSC function. It did not exhibit a consistent senolytic effect as evidenced by SAβ-gal and live dead staining, hindered proliferation in the short term in some donors, and lowered the expression of osteogenic markers COL1A1 and ALP. Quercetin treatment did not, however, negatively affect adipogenesis, chondrogenesis, or indoleamine 2,3 dioxygenase secretions.

Conclusion: This study contributes insight into the nature of quercetin and its effects on in vitro MSC culture and function.

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来源期刊
CiteScore
5.60
自引率
3.60%
发文量
30
审稿时长
>12 weeks
期刊介绍: The field of cellular and molecular bioengineering seeks to understand, so that we may ultimately control, the mechanical, chemical, and electrical processes of the cell. A key challenge in improving human health is to understand how cellular behavior arises from molecular-level interactions. CMBE, an official journal of the Biomedical Engineering Society, publishes original research and review papers in the following seven general areas: Molecular: DNA-protein/RNA-protein interactions, protein folding and function, protein-protein and receptor-ligand interactions, lipids, polysaccharides, molecular motors, and the biophysics of macromolecules that function as therapeutics or engineered matrices, for example. Cellular: Studies of how cells sense physicochemical events surrounding and within cells, and how cells transduce these events into biological responses. Specific cell processes of interest include cell growth, differentiation, migration, signal transduction, protein secretion and transport, gene expression and regulation, and cell-matrix interactions. Mechanobiology: The mechanical properties of cells and biomolecules, cellular/molecular force generation and adhesion, the response of cells to their mechanical microenvironment, and mechanotransduction in response to various physical forces such as fluid shear stress. Nanomedicine: The engineering of nanoparticles for advanced drug delivery and molecular imaging applications, with particular focus on the interaction of such particles with living cells. Also, the application of nanostructured materials to control the behavior of cells and biomolecules.
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