{"title":"当归多糖对毛鼠生长性能、抗氧化能力、免疫反应、肠道菌群和肝脏转录组的影响","authors":"Zike Yan, Jingu Shi, Kaijie Lin, Yuhan Li, Yutian Zhan, Mingzuo Gao, Hongyao Zhang, Shuyu Han, Jingzhen Liang","doi":"10.1007/s10499-025-02240-0","DOIUrl":null,"url":null,"abstract":"<div><p>The yellow pond turtle (<i>Mauremys mutica</i>), an important freshwater species in Chinese aquaculture, faces increasing threats from bacterial diseases such as <i>Klebsiella pneumoniae</i>, necessitating safe immunostimulatory strategies. <i>Angelica sinensis</i> polysaccharides (ASP), with immunomodulatory, antioxidant, and metabolic regulatory properties, are a promising feed additive. This study evaluated dietary ASP effects on growth, antioxidant capacity, immune response, intestinal microbiota, and liver transcriptome in <i>M. mutica</i>. A total of 540 turtles (150.42 ± 5.61 g) were randomly assigned to a control group (fed with basal diet, denoted as A0) and five experimental groups supplemented with 0.05%, 0.10%, 0.20%, 0.40%, and 0.80% ASP (A5, A10, A20, A40, and A80) for 65 days. ASP significantly reduced hepatosomatic index (HSI) (<i>P</i> = 0.001) and enhanced hepatic antioxidant enzyme activities, including superoxide dismutase (SOD) (<i>P</i> = 0.049), total antioxidant capacity (T-AOC) (<i>P</i> = 0.003), and glutathione peroxidase (GSH-PX) (<i>P</i> = 0.026); lowered malondialdehyde (MDA) (<i>P</i> = 0.002); decreased serum total cholesterol (TC) (<i>P</i> = 0.009) and triglycerides (TG) (<i>P</i> = 0.006); and elevated serum immune Markers, including complement component 4 (C4) (<i>P</i> < 0.001), acid phosphatase (ACP) (<i>P</i> = 0.011), alkaline phosphatase (AKP) (<i>P</i> < 0.001), and lysozyme (LZM) (<i>P</i> < 0.001). Regression analysis indicated an optimal supplementation of 0.17–0.28%. Following <i>K. pneumoniae</i> challenge, A20 showed the highest survival rate (73.33%) and relative percent survival (50.00%) (<i>P</i> = 0.002). ASP also increased beneficial intestinal taxa (<i>Lachnospira</i>, <i>Muribaculaceae_unclassified</i>, <i>Anaerovibrio</i>) and activated immune-related liver pathways, including hematopoietic cell lineage, pattern recognition receptors, and cell adhesion molecules, with key genes such as <i>HLA-DRA</i>, <i>CSMD1</i>, and <i>CLEC2D</i>. These findings highlight ASP as a functional feed additive for improving immunity and health in <i>M. mutica</i> aquaculture.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 6","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of Angelica sinensis polysaccharides on growth performance, antioxidant capacity, immune response, intestinal microbiota, and liver transcriptome in Mauremys mutica\",\"authors\":\"Zike Yan, Jingu Shi, Kaijie Lin, Yuhan Li, Yutian Zhan, Mingzuo Gao, Hongyao Zhang, Shuyu Han, Jingzhen Liang\",\"doi\":\"10.1007/s10499-025-02240-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The yellow pond turtle (<i>Mauremys mutica</i>), an important freshwater species in Chinese aquaculture, faces increasing threats from bacterial diseases such as <i>Klebsiella pneumoniae</i>, necessitating safe immunostimulatory strategies. <i>Angelica sinensis</i> polysaccharides (ASP), with immunomodulatory, antioxidant, and metabolic regulatory properties, are a promising feed additive. This study evaluated dietary ASP effects on growth, antioxidant capacity, immune response, intestinal microbiota, and liver transcriptome in <i>M. mutica</i>. A total of 540 turtles (150.42 ± 5.61 g) were randomly assigned to a control group (fed with basal diet, denoted as A0) and five experimental groups supplemented with 0.05%, 0.10%, 0.20%, 0.40%, and 0.80% ASP (A5, A10, A20, A40, and A80) for 65 days. ASP significantly reduced hepatosomatic index (HSI) (<i>P</i> = 0.001) and enhanced hepatic antioxidant enzyme activities, including superoxide dismutase (SOD) (<i>P</i> = 0.049), total antioxidant capacity (T-AOC) (<i>P</i> = 0.003), and glutathione peroxidase (GSH-PX) (<i>P</i> = 0.026); lowered malondialdehyde (MDA) (<i>P</i> = 0.002); decreased serum total cholesterol (TC) (<i>P</i> = 0.009) and triglycerides (TG) (<i>P</i> = 0.006); and elevated serum immune Markers, including complement component 4 (C4) (<i>P</i> < 0.001), acid phosphatase (ACP) (<i>P</i> = 0.011), alkaline phosphatase (AKP) (<i>P</i> < 0.001), and lysozyme (LZM) (<i>P</i> < 0.001). Regression analysis indicated an optimal supplementation of 0.17–0.28%. Following <i>K. pneumoniae</i> challenge, A20 showed the highest survival rate (73.33%) and relative percent survival (50.00%) (<i>P</i> = 0.002). ASP also increased beneficial intestinal taxa (<i>Lachnospira</i>, <i>Muribaculaceae_unclassified</i>, <i>Anaerovibrio</i>) and activated immune-related liver pathways, including hematopoietic cell lineage, pattern recognition receptors, and cell adhesion molecules, with key genes such as <i>HLA-DRA</i>, <i>CSMD1</i>, and <i>CLEC2D</i>. These findings highlight ASP as a functional feed additive for improving immunity and health in <i>M. mutica</i> aquaculture.</p></div>\",\"PeriodicalId\":8122,\"journal\":{\"name\":\"Aquaculture International\",\"volume\":\"33 6\",\"pages\":\"\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2025-09-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Aquaculture International\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10499-025-02240-0\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture International","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s10499-025-02240-0","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FISHERIES","Score":null,"Total":0}
Effects of Angelica sinensis polysaccharides on growth performance, antioxidant capacity, immune response, intestinal microbiota, and liver transcriptome in Mauremys mutica
The yellow pond turtle (Mauremys mutica), an important freshwater species in Chinese aquaculture, faces increasing threats from bacterial diseases such as Klebsiella pneumoniae, necessitating safe immunostimulatory strategies. Angelica sinensis polysaccharides (ASP), with immunomodulatory, antioxidant, and metabolic regulatory properties, are a promising feed additive. This study evaluated dietary ASP effects on growth, antioxidant capacity, immune response, intestinal microbiota, and liver transcriptome in M. mutica. A total of 540 turtles (150.42 ± 5.61 g) were randomly assigned to a control group (fed with basal diet, denoted as A0) and five experimental groups supplemented with 0.05%, 0.10%, 0.20%, 0.40%, and 0.80% ASP (A5, A10, A20, A40, and A80) for 65 days. ASP significantly reduced hepatosomatic index (HSI) (P = 0.001) and enhanced hepatic antioxidant enzyme activities, including superoxide dismutase (SOD) (P = 0.049), total antioxidant capacity (T-AOC) (P = 0.003), and glutathione peroxidase (GSH-PX) (P = 0.026); lowered malondialdehyde (MDA) (P = 0.002); decreased serum total cholesterol (TC) (P = 0.009) and triglycerides (TG) (P = 0.006); and elevated serum immune Markers, including complement component 4 (C4) (P < 0.001), acid phosphatase (ACP) (P = 0.011), alkaline phosphatase (AKP) (P < 0.001), and lysozyme (LZM) (P < 0.001). Regression analysis indicated an optimal supplementation of 0.17–0.28%. Following K. pneumoniae challenge, A20 showed the highest survival rate (73.33%) and relative percent survival (50.00%) (P = 0.002). ASP also increased beneficial intestinal taxa (Lachnospira, Muribaculaceae_unclassified, Anaerovibrio) and activated immune-related liver pathways, including hematopoietic cell lineage, pattern recognition receptors, and cell adhesion molecules, with key genes such as HLA-DRA, CSMD1, and CLEC2D. These findings highlight ASP as a functional feed additive for improving immunity and health in M. mutica aquaculture.
期刊介绍：
Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture.
The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more.
This is the official Journal of the European Aquaculture Society.
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