耐高温CNTs@Au纳米复合材料用于两种天然叶酸的高温辅助SERS分子指纹

IF 6 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta Pub Date : 2025-09-18 DOI:10.1016/j.aca.2025.344678

Zhou Yang , Lingling Dai , Fangfei Liu , Kun Jia , Zhiyuan Ren , Yanmei Yang , Qingmeng Zhang , Weifeng Li , Ming Chen

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引用次数: 0

摘要

叶酸是一种必需营养素，但其生物利用度因食物中的分子形式而异。摄入不易被人体吸收的形式可能导致叶酸水平不足，特别是在怀孕期间，从而增加出生缺陷的风险。因此，必须开发一种高度敏感和准确的方法，能够区分两种最常见的天然叶酸，5-甲基四氢叶酸（5-MTHF）和5-甲酰四氢叶酸（5-FTHF），以确保适当的叶酸摄入量和健康结果。结果本研究采用光化学方法将金纳米粒子（Au NPs）负载到碳纳米管（CNTs）上，成功合成了CNTs@Au纳米复合材料，该材料具有优异的热稳定性和强大的表面增强拉曼散射（SERS）活性。这些纳米复合材料能够在高达400°C的温度下保持结构完整性，可作为原位高温SERS检测的高效衬底。利用这种可靠的高温辅助SERS方法，可以有效地监测两种天然叶酸在高温下的结构变化，从而实现清晰的分子指纹图谱和两种天然叶酸的明显区分。此外，CNTs@Au NPs表现出卓越的灵敏度，对5-MTHF和5-FTHF的检测限低至10-8 M，能够准确定量混合叶酸样品，并在实际样品分析中表现出可靠的性能。具有原位高温SERS能力的CNTs@Au NPs在各种分析应用中显示出巨大的潜力。特别是，它们为监测膳食补充剂和强化食品中叶酸的质量提供了一种有效的方法，从而为市场上叶酸产品的安全和质量保证做出了重大贡献。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Thermostable CNTs@Au NPs nanocomposites for high-temperature assisted SERS molecular fingerprinting of two natural folates

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Thermostable CNTs@Au NPs nanocomposites for high-temperature assisted SERS molecular fingerprinting of two natural folates

Background

Folate is an essential nutrient, but its bioavailability varies greatly depending on the molecular form present in food. The intake of forms that are not readily absorbed by the human body may lead to insufficient folate levels, particularly during pregnancy, thereby increasing the risk of birth defects. As a result, it is essential to develop a highly sensitive and accurate method capable of distinguishing between the two most common natural folates, 5-methyltetrahydrofolate (5-MTHF) and 5-formyltetrahydrofolate (5-FTHF), to ensure proper folate intake and health outcomes.

Results

In this study, a photochemical method was employed to load gold nanoparticles (Au NPs) onto carbon nanotubes (CNTs), successfully synthesizing CNTs@Au NPs nanocomposites that exhibit excellent thermal stability and robust surface-enhanced Raman scattering (SERS) activity. These nanocomposites, capable of maintaining structural integrity at temperatures as high as 400 °C, serve as highly effective substrates for in situ high-temperature SERS detection. Using this reliable high-temperature-assisted SERS approach, the structural changes of both folates at elevated temperatures were effectively monitored, enabling clear molecular fingerprinting and distinct differentiation between the two natural folates. Moreover, the CNTs@Au NPs demonstrate exceptional sensitivity, achieving a detection limit as low as 10⁻⁸ M for both 5-MTHF and 5-FTHF, enabling accurate quantification of mixed folate samples and demonstrating reliable performance in real sample analysis.

Significance

The CNTs@Au NPs with in situ high-temperature SERS capabilities show great potential for various analytical applications. In particular, they provide an effective method for monitoring the quality of folate in dietary supplements and fortified foods, thus contributing significantly to the safety and quality assurance of folate products in the market.

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来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.