Fabiana de Campos Gomes, Beatriz Pavarino Bertelli, Conceição Pinheiro de Souza, Daniel Ramos de Oliveira Santos, João Simão de Melo-Neto, Érika Cristina Pavarino, Eny Maria Goloni-Bertollo
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MiRNAs were identified in the miRbase database and analysis of their potential regulation on DEGs was performed using the DIANA tools.</p><p><strong>Results: </strong><i>HSPE1</i>, <i>HSP90B1</i>, <i>HSPB8</i> and <i>HSPA13</i> genes showed a different expression pattern in the transcriptomes of DS. The <i>HSPA13</i> and <i>HSPA2</i> genes showed an altered expression profile in the DS and AD datasets. In the predicted protein-protein interactions (PPI), we identified the interaction of HSPE1, HSP90B1, HSPB8 and HSPA13 with other HSP proteins. The miRNA encoded by Hsa21 (hsa-miR-155-5p) interacted with the <i>HSPA13</i> gene.</p><p><strong>Conclusion: </strong>The results suggest that certain genes encoding members of the <i>HSP</i> family, and in particular the interaction between miR-155-5p and <i>HSPA13</i>, may be associated with AD in DS.</p>","PeriodicalId":39167,"journal":{"name":"Dementia e Neuropsychologia","volume":"19 Suppl 1","pages":"e20240239"},"PeriodicalIF":0.0000,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12435772/pdf/","citationCount":"0","resultStr":"{\"title\":\"<i>HSPA13</i> gene and microRNA-155: relationship between Down syndrome and Alzheimer's disease.\",\"authors\":\"Fabiana de Campos Gomes, Beatriz Pavarino Bertelli, Conceição Pinheiro de Souza, Daniel Ramos de Oliveira Santos, João Simão de Melo-Neto, Érika Cristina Pavarino, Eny Maria Goloni-Bertollo\",\"doi\":\"10.1590/1980-5764-DN-2024-0239\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Gene dysregulation in trisomy 21 can cause disorders of genes that are members of the heat-shock proteins (HSPs) family and contribute to the early onset of Alzheimer's disease (AD) in Down syndrome (DS).</p><p><strong>Objective: </strong>Investigate <i>in silico</i> differently expressed genes (DEGs) of HSPs and the interaction with microRNAs (miRNAs) located in human chromosome 21 (Hsa21).</p><p><strong>Methods: </strong>Two transcriptome libraries of human brain samples, datasets GSE5390 (DS) and GSE33000 (DA), were extracted from the Gene Expression Omnibus (GEO) and analyzed via GEO2R. DEGs with p-values (Adj p-values) <0.05 were analyzed via STRING. MiRNAs were identified in the miRbase database and analysis of their potential regulation on DEGs was performed using the DIANA tools.</p><p><strong>Results: </strong><i>HSPE1</i>, <i>HSP90B1</i>, <i>HSPB8</i> and <i>HSPA13</i> genes showed a different expression pattern in the transcriptomes of DS. The <i>HSPA13</i> and <i>HSPA2</i> genes showed an altered expression profile in the DS and AD datasets. In the predicted protein-protein interactions (PPI), we identified the interaction of HSPE1, HSP90B1, HSPB8 and HSPA13 with other HSP proteins. 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引用次数: 0
摘要
21三体中的基因失调可导致热休克蛋白（HSPs）家族成员基因的紊乱，并有助于唐氏综合症（DS）中阿尔茨海默病（AD）的早期发病。目的：探讨热休克蛋白（HSPs）在计算机上的不同表达基因（DEGs）及其与人类21号染色体（Hsa21）微rna （miRNAs）的相互作用。方法：从Gene Expression Omnibus （GEO）中提取人脑样本的两个转录组文库，数据集GSE5390 （DS）和GSE33000 (DA)，并通过GEO2R进行分析。结果：HSPE1、HSP90B1、HSPB8和HSPA13基因在DS转录组中表现出不同的表达模式。HSPA13和HSPA2基因在DS和AD数据集中的表达谱发生了改变。在预测的蛋白-蛋白相互作用（PPI）中，我们发现了HSPE1、HSP90B1、HSPB8和HSPA13与其他HSP蛋白的相互作用。Hsa21编码的miRNA （hsa-miR-155-5p）与HSPA13基因相互作用。结论：结果提示，编码HSP家族成员的某些基因，特别是miR-155-5p与HSPA13之间的相互作用，可能与DS中的AD有关。
HSPA13 gene and microRNA-155: relationship between Down syndrome and Alzheimer's disease.
Gene dysregulation in trisomy 21 can cause disorders of genes that are members of the heat-shock proteins (HSPs) family and contribute to the early onset of Alzheimer's disease (AD) in Down syndrome (DS).
Objective: Investigate in silico differently expressed genes (DEGs) of HSPs and the interaction with microRNAs (miRNAs) located in human chromosome 21 (Hsa21).
Methods: Two transcriptome libraries of human brain samples, datasets GSE5390 (DS) and GSE33000 (DA), were extracted from the Gene Expression Omnibus (GEO) and analyzed via GEO2R. DEGs with p-values (Adj p-values) <0.05 were analyzed via STRING. MiRNAs were identified in the miRbase database and analysis of their potential regulation on DEGs was performed using the DIANA tools.
Results: HSPE1, HSP90B1, HSPB8 and HSPA13 genes showed a different expression pattern in the transcriptomes of DS. The HSPA13 and HSPA2 genes showed an altered expression profile in the DS and AD datasets. In the predicted protein-protein interactions (PPI), we identified the interaction of HSPE1, HSP90B1, HSPB8 and HSPA13 with other HSP proteins. The miRNA encoded by Hsa21 (hsa-miR-155-5p) interacted with the HSPA13 gene.
Conclusion: The results suggest that certain genes encoding members of the HSP family, and in particular the interaction between miR-155-5p and HSPA13, may be associated with AD in DS.
期刊介绍：
Dementia top Neuropsychologia the official scientific journal of the Cognitive Neurology and Ageing Department of the Brazilian Academy of Neurology and of the Brazilian Association of Geriatric Neuropsychiatry, is published by the "Associação Neurologia Cognitiva e do Comportamento", a nonprofit Brazilian association. Regularly published on March, June, September, and December since 2007.
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