桦树BpYAB1的功能特征:过表达导致叶片卷曲、发育缺陷和水分流失增加。

IF 3.6 2区 生物学 Q1 PLANT SCIENCES
Baoyue Xing, Tianxu Zhang, Huiyu Li, Jing Jiang, Rui Han, Guanbin Lv, Kun Chen, Guifeng Liu
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引用次数: 0

摘要

叶片是植物重要的光合器官,在植物生长发育中起着至关重要的作用。转录因子YABBY (YAB)家族在叶片发育中尤为重要,它调节叶片背面极性的建立。在本研究中,我们分析了BpYAB1在木本树种白桦(Betula platyphylla)中的表达。BpYAB1定位于细胞核,在顶芽中表达量最高,从第1叶到第4叶、茎和根的表达量逐渐降低。为了进一步阐明BpYAB1的功能,我们构建了转基因白桦株系。虽然BpYAB1基因敲除的桦树品系与野生型(WT)相比没有显著的表型差异,但BpYAB1过表达诱导叶片背面卷曲,并伴随上下表皮、栅栏/海绵组织、维管木质部和地面组织的结构改变。此外,过表达bpyab1的细胞系表现出发育迟缓和水分损失率增加。转录组分析表明,这些表型缺陷可能与生长素反应基因、生长相关基因和应激反应基因的表达改变有关。总的来说,我们的发现为白桦叶片发育的分子机制提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Functional Characterization of BpYAB1 in Birch: Overexpression Leads to Leaf Curling, Developmental Defects, and Elevated Water Loss.

Leaves are vital photosynthetic organs in plants, playing crucial roles in growth and development. The transcription factor YABBY (YAB) family is particularly important in leaf development, where it regulates abaxial polarity establishment. In this study, we analyzed BpYAB1 expression in the woody species Betula platyphylla. BpYAB1 was localized to the nucleus and showed the highest expression in apical buds, with gradually reduced levels from the 1st to the 4th leaves, stems, and roots. To further elucidate BpYAB1 function, we generated transgenic B. platyphylla lines. While BpYAB1 knockout birch lines showed no significant phenotypic differences compared to wild-type (WT), BpYAB1 overexpression induced abaxial leaf curling, accompanied by structural alterations in the upper/lower epidermis, palisade/spongy tissues, vascular xylem, and ground tissue. Additionally, BpYAB1-overexpressing lines displayed developmental delays and increased water loss rates. Transcriptome analysis suggested that these phenotypic defects may be linked to altered expression of auxin-responsive, growth-related, and stress-response genes. Collectively, our findings provide novel insights into the molecular mechanisms governing leaf development in B. platyphylla.

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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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