torvum粗提物对重要分枝杆菌的抑菌活性研究

IF 1.5 Q4 INFECTIOUS DISEASES
International Journal of Mycobacteriology Pub Date : 2025-07-01 Epub Date: 2025-09-15 DOI:10.4103/ijmy.ijmy_69_25
Joseph Mwanzia Nguta
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引用次数: 0

摘要

背景:结核分枝杆菌(M. TB)复合体引起的结核病(TB)仍然是世界范围内发病率和死亡率的主要原因。人畜共患传染病是一个永无止境的挑战,需要药物发现的努力。目前的研究旨在评估从torvum(一种传统上用于治疗包括结核病在内的呼吸道疾病的灌木)的根、茎、皮、叶和未成熟果实中提取的氢乙醇提取物的体外抗细菌活性和植物化学成分。方法:采用表型比色微孔板alamar蓝法研究水乙醇提取物对6株分枝杆菌的抑菌活性。每个实验都做了三遍。使用描述性统计分析生成的数据以获得平均最小抑制浓度(MIC)值。结果:根、茎皮、叶和未成熟果实对结核分枝杆菌H37Rv (ATCC 27294)的MIC值分别为0.0195 mg/mL、0.0781 mg/mL、1.250 mg/mL和0.625 mg/mL。结论:torvum茎皮对致病性结核分枝杆菌具有明显的抑制作用。这一观察结果证实了该植物抗结核的民族药理学用途。进一步的研究需要分离,阐明结构,并表征抗细菌化合物负责观察到的活性。这将有助于生物勘探一类具有抗结核分枝杆菌敏感和耐药菌株活性的新型配体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antimicrobial Activity of Solanum torvum Crude Extracts against Important Mycobacterial Strains.

Background: Tuberculosis (TB) caused by Mycobacterium tuberculosis (M. tb) complex remains a leading cause of morbidity and mortality worldwide. The zoonotic infectious condition represents a never-ending challenge for which drug discovery efforts are needed. The current study was designed to evaluate the in vitro antimycobacterial activity and phytochemical composition of hydroethanolic extracts from roots, stem bark, leaves, and unripe fruits derived from Solanum torvum, a shrub traditionally used against respiratory tract illnesses, including TB.

Methods: The phenotypic colorimetric microplate alamar blue assay was used to study the antimycobacterial activity of the hydroethanolic extracts against six mycobacterial strains. Each experiment was run in triplicate. Data generated were analyzed using descriptive statistics to obtain mean minimum inhibitory concentration (MIC) values.

Results: The roots, stem bark, leaves, and unripe fruits exhibited MIC values of 0.0195 mg/mL, 0.0781 mg/mL, 1.250 mg/mL, and 0.625 mg/mL against the pathogenic mycobacterial strain, M. tb H37Rv (ATCC 27294), respectively.

Conclusions: S. torvum stem bark has demonstrated significant activity against the pathogenic M. tb strain. This observation validates the ethno-pharmacological use of the plant species against TB. Further studies are required to isolate, elucidate the structure, and characterize the antimycobacterial compounds responsible for the observed activity. These will potentially contribute toward bioprospecting for a new class of ligands with activity against sensitive and drug-resistant strains of M. tb.

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来源期刊
CiteScore
2.20
自引率
25.00%
发文量
62
审稿时长
7 weeks
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