新型杂交TPGS植物体增强葡萄籽提取物抗光老化效果的制备及深入分析。

IF 4.3 2区医学 Q1 PHARMACOLOGY & PHARMACY

European Journal of Pharmaceutics and Biopharmaceutics Pub Date : 2025-09-13 DOI:10.1016/j.ejpb.2025.114843

Hanan Refai , Mona Elhabak , Doaa H. Hassan , Omar S. Ahmed , Esraa M. Mohamed , Heba S. Rateb , Nesrine S. El Sayed , Mariam K. Ahmed , Pierre Waffo-Téguo , Josep Valls , Nermeen Z. Abuelezz

{"title":"新型杂交TPGS植物体增强葡萄籽提取物抗光老化效果的制备及深入分析。","authors":"Hanan Refai , Mona Elhabak , Doaa H. Hassan , Omar S. Ahmed , Esraa M. Mohamed , Heba S. Rateb , Nesrine S. El Sayed , Mariam K. Ahmed , Pierre Waffo-Téguo , Josep Valls , Nermeen Z. Abuelezz","doi":"10.1016/j.ejpb.2025.114843","DOIUrl":null,"url":null,"abstract":"<div><div>Photoaging is a widespread skin health concern caused by UV radiation. It significantly contributes to premature wrinkles and exacerbates further skin damage. This study evaluates the enhanced anti-aging efficacy of a nano phytosome incorporating grape seed extract (GSE) as an innovative, naturally driven antiaging formula, demonstrating putative skin permeability. LC-HRMS analysis and molecular docking revealed the potential of GSE components against photoaging targets. A GSE-nano phytosome formula with phosphatidyl choline (PC)/GSE ratio of 50:1 and 10 mg Vitamin E TPGS displayed optimal physicochemical properties, including particle size (195.1 ± 0.4 nm), polydispersity index (0.312 ± 0.015), zeta potential (25.6 ± 0.4 mV), and complexation efficiency (76.2 ± 4.5 %). FTIR analysis confirmed the formation of hybrid phytosomes through complexation with PC and TPGS. <em>ex vivo</em> confocal microscopy displayed enhanced skin permeability. Biochemical studies confirmed the superior antiaging effect of GSE-phytosome(GSE-nanogel) compared to crude GSE in UV-irradiated rats. GSE nanogel promoted anti-aging by suppressing c-Jun N-terminal kinase (JNK) and NF-κB, caspase-3, enhancing GSH-Px level and reducing malondialdehyde level. These effects suppressed elastase and collagenase activity, significantly improving wrinkles and skin redness. Our findings underscore the efficiency of formulating high molecular weight GSE phytoconstituents as phytosomes that mimic the lipophilic nature of cell membranes, improve skin permeability, and therapeutic efficacy.</div></div>","PeriodicalId":12024,"journal":{"name":"European Journal of Pharmaceutics and Biopharmaceutics","volume":"216 ","pages":"Article 114843"},"PeriodicalIF":4.3000,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Fabrication and in-depth analysis of a novel-hybrid TPGS phytosome system for enhanced anti photoaging efficacy of grape seed extract\",\"authors\":\"Hanan Refai , Mona Elhabak , Doaa H. Hassan , Omar S. Ahmed , Esraa M. Mohamed , Heba S. Rateb , Nesrine S. El Sayed , Mariam K. Ahmed , Pierre Waffo-Téguo , Josep Valls , Nermeen Z. Abuelezz\",\"doi\":\"10.1016/j.ejpb.2025.114843\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Photoaging is a widespread skin health concern caused by UV radiation. It significantly contributes to premature wrinkles and exacerbates further skin damage. This study evaluates the enhanced anti-aging efficacy of a nano phytosome incorporating grape seed extract (GSE) as an innovative, naturally driven antiaging formula, demonstrating putative skin permeability. LC-HRMS analysis and molecular docking revealed the potential of GSE components against photoaging targets. A GSE-nano phytosome formula with phosphatidyl choline (PC)/GSE ratio of 50:1 and 10 mg Vitamin E TPGS displayed optimal physicochemical properties, including particle size (195.1 ± 0.4 nm), polydispersity index (0.312 ± 0.015), zeta potential (25.6 ± 0.4 mV), and complexation efficiency (76.2 ± 4.5 %). FTIR analysis confirmed the formation of hybrid phytosomes through complexation with PC and TPGS. <em>ex vivo</em> confocal microscopy displayed enhanced skin permeability. Biochemical studies confirmed the superior antiaging effect of GSE-phytosome(GSE-nanogel) compared to crude GSE in UV-irradiated rats. GSE nanogel promoted anti-aging by suppressing c-Jun N-terminal kinase (JNK) and NF-κB, caspase-3, enhancing GSH-Px level and reducing malondialdehyde level. These effects suppressed elastase and collagenase activity, significantly improving wrinkles and skin redness. Our findings underscore the efficiency of formulating high molecular weight GSE phytoconstituents as phytosomes that mimic the lipophilic nature of cell membranes, improve skin permeability, and therapeutic efficacy.</div></div>\",\"PeriodicalId\":12024,\"journal\":{\"name\":\"European Journal of Pharmaceutics and Biopharmaceutics\",\"volume\":\"216 \",\"pages\":\"Article 114843\"},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2025-09-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"European Journal of Pharmaceutics and Biopharmaceutics\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0939641125002206\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Pharmaceutics and Biopharmaceutics","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0939641125002206","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}

引用次数: 0

摘要

光老化是由紫外线辐射引起的一种广泛的皮肤健康问题。它会导致过早的皱纹，并进一步加剧皮肤损伤。本研究评估了一种含有葡萄籽提取物（GSE）的纳米植物体作为一种创新的，自然驱动的抗衰老配方的增强抗衰老功效，证明了假定的皮肤渗透性。LC-HRMS分析和分子对接揭示了GSE组分对抗光老化靶标的潜力。一个GSE-nano phytosome公式与磷脂酰胆碱(PC) / GSE比为50:1和10 毫克维生素E tpg显示最佳的物理化学性质,包括颗粒大小(195.1 ±0.4  海里),多分散性指数(0.312 ±0.015 ),电动电势(25.6 ±0.4  mV),和络合效率(76.2 ±4.5  %)。FTIR分析证实了通过与PC和TPGS络合形成杂交磷脂质体。体外共聚焦显微镜显示皮肤通透性增强。生化研究证实，与粗GSE相比，GSE-光敏体（GSE-纳米凝胶）对紫外线照射大鼠的抗衰老作用更强。GSE纳米凝胶通过抑制c-Jun n -末端激酶（JNK）和NF-κB、caspase-3，提高GSH-Px水平，降低丙二醛水平，促进抗衰老。这些效果抑制弹性酶和胶原酶活性，显著改善皱纹和皮肤红肿。我们的研究结果强调了高分子量GSE植物成分作为磷脂小体的效率，这些磷脂小体可以模拟细胞膜的亲脂性，提高皮肤的渗透性和治疗效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Fabrication and in-depth analysis of a novel-hybrid TPGS phytosome system for enhanced anti photoaging efficacy of grape seed extract

查看原文本刊更多论文

Fabrication and in-depth analysis of a novel-hybrid TPGS phytosome system for enhanced anti photoaging efficacy of grape seed extract

Photoaging is a widespread skin health concern caused by UV radiation. It significantly contributes to premature wrinkles and exacerbates further skin damage. This study evaluates the enhanced anti-aging efficacy of a nano phytosome incorporating grape seed extract (GSE) as an innovative, naturally driven antiaging formula, demonstrating putative skin permeability. LC-HRMS analysis and molecular docking revealed the potential of GSE components against photoaging targets. A GSE-nano phytosome formula with phosphatidyl choline (PC)/GSE ratio of 50:1 and 10 mg Vitamin E TPGS displayed optimal physicochemical properties, including particle size (195.1 ± 0.4 nm), polydispersity index (0.312 ± 0.015), zeta potential (25.6 ± 0.4 mV), and complexation efficiency (76.2 ± 4.5 %). FTIR analysis confirmed the formation of hybrid phytosomes through complexation with PC and TPGS. ex vivo confocal microscopy displayed enhanced skin permeability. Biochemical studies confirmed the superior antiaging effect of GSE-phytosome(GSE-nanogel) compared to crude GSE in UV-irradiated rats. GSE nanogel promoted anti-aging by suppressing c-Jun N-terminal kinase (JNK) and NF-κB, caspase-3, enhancing GSH-Px level and reducing malondialdehyde level. These effects suppressed elastase and collagenase activity, significantly improving wrinkles and skin redness. Our findings underscore the efficiency of formulating high molecular weight GSE phytoconstituents as phytosomes that mimic the lipophilic nature of cell membranes, improve skin permeability, and therapeutic efficacy.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

European Journal of Pharmaceutics and Biopharmaceutics 医学-药学

CiteScore

8.80

自引率

4.10%

发文量

211

审稿时长

36 days

期刊介绍： The European Journal of Pharmaceutics and Biopharmaceutics provides a medium for the publication of novel, innovative and hypothesis-driven research from the areas of Pharmaceutics and Biopharmaceutics. Topics covered include for example: Design and development of drug delivery systems for pharmaceuticals and biopharmaceuticals (small molecules, proteins, nucleic acids) Aspects of manufacturing process design Biomedical aspects of drug product design Strategies and formulations for controlled drug transport across biological barriers Physicochemical aspects of drug product development Novel excipients for drug product design Drug delivery and controlled release systems for systemic and local applications Nanomaterials for therapeutic and diagnostic purposes Advanced therapy medicinal products Medical devices supporting a distinct pharmacological effect.