{"title":"污水源好氧甲烷氧化菌的富集及应用以提高污水中气态甲烷的去除","authors":"Xin Yuan , Zhiqiang Zuo , Yuqi Yang , Yanyan Zhang , Xing Xin , Jiane Zuo","doi":"10.1016/j.envint.2025.109798","DOIUrl":null,"url":null,"abstract":"<div><div>Methane emission from sewer systems persists as a dual environmental threat, combining explosion risks with potent greenhouse gas impacts. Current methane removal strategies have primarily targeted biofilm and wastewater phases by chemical dosing; however, microbial adaptation and fluctuating water quality often reduce the control efficiency, incurring high chemical costs. Here we propose and demonstrate a novel microbial approach that utilizes aerobic methanotrophs for direct gaseous methane oxidation. Sewer-derived microbes was discovered to exhibit a high methane oxidation capacity of 0.30 μmol/(h·g). The sewer-derived methanotrophs were then successfully enriched, with a high methane oxidation rate of more than 200 mg CH<sub>4</sub>/(g DCW·d). Leveraging the enriched aerobic methanotrophs, an innovative in-situ gaseous methane control strategy was developed. Methane emission reduction reached 85.6 % in a sewer reactor under prolonged operation, and microbial activity could be restored following easy and flexible reactivation treatment. Notably, hydrogen sulfide could be eliminated in this reactor, attributed to biochar-mediated adsorption and subsequent chemolithotrophic oxidation, which demonstrates the multifunctionality of the hybrid system. High-throughput sequencing and metagenomic sequencing further revealed <em>Methylocystis</em> (15 %-20 % relative abundance) and <em>Methylophilus</em> (17 %-46 % relative abundance) as core functional species that exhibit significant potential for carbon emission reduction in sewer systems. These results can demonstrate an economical and environmentally friendly solution to both gaseous methane and hydrogen sulfide removal in sewers.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"204 ","pages":"Article 109798"},"PeriodicalIF":9.7000,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enrichment and application of sewer-derived aerobic methanotrophs to enhance gaseous methane removal in sewers\",\"authors\":\"Xin Yuan , Zhiqiang Zuo , Yuqi Yang , Yanyan Zhang , Xing Xin , Jiane Zuo\",\"doi\":\"10.1016/j.envint.2025.109798\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Methane emission from sewer systems persists as a dual environmental threat, combining explosion risks with potent greenhouse gas impacts. Current methane removal strategies have primarily targeted biofilm and wastewater phases by chemical dosing; however, microbial adaptation and fluctuating water quality often reduce the control efficiency, incurring high chemical costs. Here we propose and demonstrate a novel microbial approach that utilizes aerobic methanotrophs for direct gaseous methane oxidation. Sewer-derived microbes was discovered to exhibit a high methane oxidation capacity of 0.30 μmol/(h·g). The sewer-derived methanotrophs were then successfully enriched, with a high methane oxidation rate of more than 200 mg CH<sub>4</sub>/(g DCW·d). Leveraging the enriched aerobic methanotrophs, an innovative in-situ gaseous methane control strategy was developed. Methane emission reduction reached 85.6 % in a sewer reactor under prolonged operation, and microbial activity could be restored following easy and flexible reactivation treatment. Notably, hydrogen sulfide could be eliminated in this reactor, attributed to biochar-mediated adsorption and subsequent chemolithotrophic oxidation, which demonstrates the multifunctionality of the hybrid system. High-throughput sequencing and metagenomic sequencing further revealed <em>Methylocystis</em> (15 %-20 % relative abundance) and <em>Methylophilus</em> (17 %-46 % relative abundance) as core functional species that exhibit significant potential for carbon emission reduction in sewer systems. These results can demonstrate an economical and environmentally friendly solution to both gaseous methane and hydrogen sulfide removal in sewers.</div></div>\",\"PeriodicalId\":308,\"journal\":{\"name\":\"Environment International\",\"volume\":\"204 \",\"pages\":\"Article 109798\"},\"PeriodicalIF\":9.7000,\"publicationDate\":\"2025-09-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environment International\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0160412025005495\",\"RegionNum\":1,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environment International","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0160412025005495","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Enrichment and application of sewer-derived aerobic methanotrophs to enhance gaseous methane removal in sewers
Methane emission from sewer systems persists as a dual environmental threat, combining explosion risks with potent greenhouse gas impacts. Current methane removal strategies have primarily targeted biofilm and wastewater phases by chemical dosing; however, microbial adaptation and fluctuating water quality often reduce the control efficiency, incurring high chemical costs. Here we propose and demonstrate a novel microbial approach that utilizes aerobic methanotrophs for direct gaseous methane oxidation. Sewer-derived microbes was discovered to exhibit a high methane oxidation capacity of 0.30 μmol/(h·g). The sewer-derived methanotrophs were then successfully enriched, with a high methane oxidation rate of more than 200 mg CH4/(g DCW·d). Leveraging the enriched aerobic methanotrophs, an innovative in-situ gaseous methane control strategy was developed. Methane emission reduction reached 85.6 % in a sewer reactor under prolonged operation, and microbial activity could be restored following easy and flexible reactivation treatment. Notably, hydrogen sulfide could be eliminated in this reactor, attributed to biochar-mediated adsorption and subsequent chemolithotrophic oxidation, which demonstrates the multifunctionality of the hybrid system. High-throughput sequencing and metagenomic sequencing further revealed Methylocystis (15 %-20 % relative abundance) and Methylophilus (17 %-46 % relative abundance) as core functional species that exhibit significant potential for carbon emission reduction in sewer systems. These results can demonstrate an economical and environmentally friendly solution to both gaseous methane and hydrogen sulfide removal in sewers.
期刊介绍:
Environmental Health publishes manuscripts focusing on critical aspects of environmental and occupational medicine, including studies in toxicology and epidemiology, to illuminate the human health implications of exposure to environmental hazards. The journal adopts an open-access model and practices open peer review.
It caters to scientists and practitioners across all environmental science domains, directly or indirectly impacting human health and well-being. With a commitment to enhancing the prevention of environmentally-related health risks, Environmental Health serves as a public health journal for the community and scientists engaged in matters of public health significance concerning the environment.