Dr Indumathi N, Dr Nandhini G, Dr Rajkumar K, Dr Poonguzhalnalli K
{"title":"YAP1在口腔白斑和鳞状细胞癌中表达的免疫组化评价：对预后的影响","authors":"Dr Indumathi N, Dr Nandhini G, Dr Rajkumar K, Dr Poonguzhalnalli K","doi":"10.1016/j.jormas.2025.102547","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Oral Squamous Cell Carcinoma (OSCC), often preceded by epithelial dysplasia, is a prevalent head and neck malignancy. Yes-associated protein1 (YAP1), a key effector of the Hippo pathway, functions as an oncogene promoting cell proliferation and tumorigenesis. Elevated YAP1 expression has been linked to poor prognosis in OSCC. This study investigates the immunohistochemical expression of YAP1 in normal oral mucosa, epithelial dysplasia, and OSCC to clarify its role in oral carcinogenesis.</p><p><strong>Aims and objectives: </strong>The present study aims to evaluate the immuno-expression of YAP1 in normal oral mucosa, epithelial dysplasia, and OSCC, with the objective of elucidating its role in tumor progression by correlating expression patterns across these groups.</p><p><strong>Methodology: </strong>Tissue samples from normal oral mucosa (n=9), epithelial dysplasia (n=9), and OSCC (n=9) were analyzed for YAP1 expression using chromogenic immunohistochemistry. Staining intensity and localization were compared across groups, and statistical significance was assessed via Chi-square test.</p><p><strong>Results: </strong>YAP1 expression significantly increased from normal mucosa to dysplasia and OSCC (p=0.001). Normal tissues predominantly showed absent or weak cytoplasmic staining, whereas dysplastic lesions exhibited mixed weak cytoplasmic and focal nuclear staining. OSCC samples demonstrated strong cytoplasmic staining in over 50% of cells, with notable nuclear localization. This progressive increase in YAP1 expression and altered subcellular distribution correlates with lesion severity, suggesting dysregulated YAP1 activity during malignant transformation.</p><p><strong>Conclusion: </strong>The findings support YAP1 as a potential biomarker for the malignant progression of Oral Epithelial Dysplasia to OSCC. Enhanced YAP1 expression and nuclear localization may reflect its oncogenic role in oral carcinogenesis. Further studies with larger cohorts and mechanistic analyses are warranted to validate YAP1's prognostic utility and explore its potential as a therapeutic target in OSCC.</p>","PeriodicalId":56038,"journal":{"name":"Journal of Stomatology Oral and Maxillofacial Surgery","volume":" ","pages":"102547"},"PeriodicalIF":2.0000,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Immunohistochemical Evaluation of Yes-associated protein1 (YAP1) Expression in Oral Leukoplakia and Squamous Cell Carcinoma: Implications for Prognosis.\",\"authors\":\"Dr Indumathi N, Dr Nandhini G, Dr Rajkumar K, Dr Poonguzhalnalli K\",\"doi\":\"10.1016/j.jormas.2025.102547\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Oral Squamous Cell Carcinoma (OSCC), often preceded by epithelial dysplasia, is a prevalent head and neck malignancy. Yes-associated protein1 (YAP1), a key effector of the Hippo pathway, functions as an oncogene promoting cell proliferation and tumorigenesis. Elevated YAP1 expression has been linked to poor prognosis in OSCC. This study investigates the immunohistochemical expression of YAP1 in normal oral mucosa, epithelial dysplasia, and OSCC to clarify its role in oral carcinogenesis.</p><p><strong>Aims and objectives: </strong>The present study aims to evaluate the immuno-expression of YAP1 in normal oral mucosa, epithelial dysplasia, and OSCC, with the objective of elucidating its role in tumor progression by correlating expression patterns across these groups.</p><p><strong>Methodology: </strong>Tissue samples from normal oral mucosa (n=9), epithelial dysplasia (n=9), and OSCC (n=9) were analyzed for YAP1 expression using chromogenic immunohistochemistry. Staining intensity and localization were compared across groups, and statistical significance was assessed via Chi-square test.</p><p><strong>Results: </strong>YAP1 expression significantly increased from normal mucosa to dysplasia and OSCC (p=0.001). Normal tissues predominantly showed absent or weak cytoplasmic staining, whereas dysplastic lesions exhibited mixed weak cytoplasmic and focal nuclear staining. OSCC samples demonstrated strong cytoplasmic staining in over 50% of cells, with notable nuclear localization. This progressive increase in YAP1 expression and altered subcellular distribution correlates with lesion severity, suggesting dysregulated YAP1 activity during malignant transformation.</p><p><strong>Conclusion: </strong>The findings support YAP1 as a potential biomarker for the malignant progression of Oral Epithelial Dysplasia to OSCC. Enhanced YAP1 expression and nuclear localization may reflect its oncogenic role in oral carcinogenesis. Further studies with larger cohorts and mechanistic analyses are warranted to validate YAP1's prognostic utility and explore its potential as a therapeutic target in OSCC.</p>\",\"PeriodicalId\":56038,\"journal\":{\"name\":\"Journal of Stomatology Oral and Maxillofacial Surgery\",\"volume\":\" \",\"pages\":\"102547\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-09-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Stomatology Oral and Maxillofacial Surgery\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.jormas.2025.102547\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Dentistry\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Stomatology Oral and Maxillofacial Surgery","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.jormas.2025.102547","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Dentistry","Score":null,"Total":0}
Immunohistochemical Evaluation of Yes-associated protein1 (YAP1) Expression in Oral Leukoplakia and Squamous Cell Carcinoma: Implications for Prognosis.
Background: Oral Squamous Cell Carcinoma (OSCC), often preceded by epithelial dysplasia, is a prevalent head and neck malignancy. Yes-associated protein1 (YAP1), a key effector of the Hippo pathway, functions as an oncogene promoting cell proliferation and tumorigenesis. Elevated YAP1 expression has been linked to poor prognosis in OSCC. This study investigates the immunohistochemical expression of YAP1 in normal oral mucosa, epithelial dysplasia, and OSCC to clarify its role in oral carcinogenesis.
Aims and objectives: The present study aims to evaluate the immuno-expression of YAP1 in normal oral mucosa, epithelial dysplasia, and OSCC, with the objective of elucidating its role in tumor progression by correlating expression patterns across these groups.
Methodology: Tissue samples from normal oral mucosa (n=9), epithelial dysplasia (n=9), and OSCC (n=9) were analyzed for YAP1 expression using chromogenic immunohistochemistry. Staining intensity and localization were compared across groups, and statistical significance was assessed via Chi-square test.
Results: YAP1 expression significantly increased from normal mucosa to dysplasia and OSCC (p=0.001). Normal tissues predominantly showed absent or weak cytoplasmic staining, whereas dysplastic lesions exhibited mixed weak cytoplasmic and focal nuclear staining. OSCC samples demonstrated strong cytoplasmic staining in over 50% of cells, with notable nuclear localization. This progressive increase in YAP1 expression and altered subcellular distribution correlates with lesion severity, suggesting dysregulated YAP1 activity during malignant transformation.
Conclusion: The findings support YAP1 as a potential biomarker for the malignant progression of Oral Epithelial Dysplasia to OSCC. Enhanced YAP1 expression and nuclear localization may reflect its oncogenic role in oral carcinogenesis. Further studies with larger cohorts and mechanistic analyses are warranted to validate YAP1's prognostic utility and explore its potential as a therapeutic target in OSCC.
期刊介绍：
J Stomatol Oral Maxillofac Surg publishes research papers and techniques - (guest) editorials, original articles, reviews, technical notes, case reports, images, letters to the editor, guidelines - dedicated to enhancing surgical expertise in all fields relevant to oral and maxillofacial surgery: from plastic and reconstructive surgery of the face, oral surgery and medicine, … to dentofacial and maxillofacial orthopedics.
Original articles include clinical or laboratory investigations and clinical or equipment reports. Reviews include narrative reviews, systematic reviews and meta-analyses.
All manuscripts submitted to the journal are subjected to peer review by international experts, and must:
Be written in excellent English, clear and easy to understand, precise and concise;
Bring new, interesting, valid information - and improve clinical care or guide future research;
Be solely the work of the author(s) stated;
Not have been previously published elsewhere and not be under consideration by another journal;
Be in accordance with the journal''s Guide for Authors'' instructions: manuscripts that fail to comply with these rules may be returned to the authors without being reviewed.
Under no circumstances does the journal guarantee publication before the editorial board makes its final decision.
The journal is indexed in the main international databases and is accessible worldwide through the ScienceDirect and ClinicalKey Platforms.
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