{"title":"MiR-21和MiR-155在口腔鳞状细胞癌中的表达：墨西哥患者FFPE活检的回顾性研究","authors":"Yolanda Terán-Figueroa, Obed Lemus-Rojero, Ángeles Catalina Ochoa-Martínez, Luz Eugenia Alcántara-Quintana, Iván N Pérez-Maldonado, Nuria Patiño-Marín, Jorge Alejandro Alegría-Torres","doi":"10.4103/jispcd.jispcd_42_25","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong>Oral squamous cell carcinoma (OSCC) is a prevalent malignancy with poor survival outcomes, particularly in regions with high tobacco use and less early detection. MicroRNAs (miRNAs) such as miR-21 and miR-155 have been implicated in tumor progression and viral oncogenesis. This study aimed to evaluate the expressions of miR-21 and miR-155 in OSCC tissues and their association with histological differentiation and HPV infection in a Mexican population.</p><p><strong>Materials and methods: </strong>This retrospective cross-sectional study included 30 OSCC cases and 30 age- and sex-matched healthy oral tissue controls. Total RNA was extracted from formalin-fixed paraffin-embedded tissue samples, and miR-21 and miR-155 levels were quantified using RT-qPCR. HPV genotyping (types 16 and 18) was performed via endpoint PCR. Statistical analysis included Mann-Whitney <i>U</i> test, Kruskal-Wallis test, and Fisher's exact test. Effect sizes were calculated using Glass's delta, and a significance level of <i>P</i> < 0.05 was applied.</p><p><strong>Results: </strong>MiR-21 and miR-155 were significantly upregulated in OSCC tissues compared to controls (<i>P</i> < 0.001 for both), with large effect sizes (Glass's <i>δ</i> = 3.78 and 2.63, respectively). No significant association was found between miRNA expression levels and tumor differentiation grade (<i>P</i> > 0.05). HPV was detected in 26.6% of OSCC samples, with HPV-16 being the most frequent subtype. A significant association was observed between HPV positivity and tobacco use (<i>P</i> = 0.002), but not with miRNA expression levels.</p><p><strong>Conclusion: </strong>The overexpression of miR-21 and miR-155 in OSCC supports their potential as diagnostic biomarkers in oral cancer. While their levels were not significantly associated with tumor grade or HPV status, their consistent elevation in malignant tissues warrants further investigation into their mechanistic role in OSCC pathogenesis and their applicability in biomarker panels for early detection, especially in Latin American populations.</p>","PeriodicalId":47247,"journal":{"name":"Journal of International Society of Preventive and Community Dentistry","volume":"15 4","pages":"374-381"},"PeriodicalIF":2.1000,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12425401/pdf/","citationCount":"0","resultStr":"{\"title\":\"MiR-21 and MiR-155 Expressions in Oral Squamous Cell Carcinoma: A Retrospective Study in Mexican Patients using FFPE Biopsies.\",\"authors\":\"Yolanda Terán-Figueroa, Obed Lemus-Rojero, Ángeles Catalina Ochoa-Martínez, Luz Eugenia Alcántara-Quintana, Iván N Pérez-Maldonado, Nuria Patiño-Marín, Jorge Alejandro Alegría-Torres\",\"doi\":\"10.4103/jispcd.jispcd_42_25\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aim: </strong>Oral squamous cell carcinoma (OSCC) is a prevalent malignancy with poor survival outcomes, particularly in regions with high tobacco use and less early detection. MicroRNAs (miRNAs) such as miR-21 and miR-155 have been implicated in tumor progression and viral oncogenesis. This study aimed to evaluate the expressions of miR-21 and miR-155 in OSCC tissues and their association with histological differentiation and HPV infection in a Mexican population.</p><p><strong>Materials and methods: </strong>This retrospective cross-sectional study included 30 OSCC cases and 30 age- and sex-matched healthy oral tissue controls. Total RNA was extracted from formalin-fixed paraffin-embedded tissue samples, and miR-21 and miR-155 levels were quantified using RT-qPCR. HPV genotyping (types 16 and 18) was performed via endpoint PCR. Statistical analysis included Mann-Whitney <i>U</i> test, Kruskal-Wallis test, and Fisher's exact test. Effect sizes were calculated using Glass's delta, and a significance level of <i>P</i> < 0.05 was applied.</p><p><strong>Results: </strong>MiR-21 and miR-155 were significantly upregulated in OSCC tissues compared to controls (<i>P</i> < 0.001 for both), with large effect sizes (Glass's <i>δ</i> = 3.78 and 2.63, respectively). No significant association was found between miRNA expression levels and tumor differentiation grade (<i>P</i> > 0.05). HPV was detected in 26.6% of OSCC samples, with HPV-16 being the most frequent subtype. A significant association was observed between HPV positivity and tobacco use (<i>P</i> = 0.002), but not with miRNA expression levels.</p><p><strong>Conclusion: </strong>The overexpression of miR-21 and miR-155 in OSCC supports their potential as diagnostic biomarkers in oral cancer. While their levels were not significantly associated with tumor grade or HPV status, their consistent elevation in malignant tissues warrants further investigation into their mechanistic role in OSCC pathogenesis and their applicability in biomarker panels for early detection, especially in Latin American populations.</p>\",\"PeriodicalId\":47247,\"journal\":{\"name\":\"Journal of International Society of Preventive and Community Dentistry\",\"volume\":\"15 4\",\"pages\":\"374-381\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2025-08-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12425401/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of International Society of Preventive and Community Dentistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/jispcd.jispcd_42_25\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/7/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of International Society of Preventive and Community Dentistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/jispcd.jispcd_42_25","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/7/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
MiR-21 and MiR-155 Expressions in Oral Squamous Cell Carcinoma: A Retrospective Study in Mexican Patients using FFPE Biopsies.
Aim: Oral squamous cell carcinoma (OSCC) is a prevalent malignancy with poor survival outcomes, particularly in regions with high tobacco use and less early detection. MicroRNAs (miRNAs) such as miR-21 and miR-155 have been implicated in tumor progression and viral oncogenesis. This study aimed to evaluate the expressions of miR-21 and miR-155 in OSCC tissues and their association with histological differentiation and HPV infection in a Mexican population.
Materials and methods: This retrospective cross-sectional study included 30 OSCC cases and 30 age- and sex-matched healthy oral tissue controls. Total RNA was extracted from formalin-fixed paraffin-embedded tissue samples, and miR-21 and miR-155 levels were quantified using RT-qPCR. HPV genotyping (types 16 and 18) was performed via endpoint PCR. Statistical analysis included Mann-Whitney U test, Kruskal-Wallis test, and Fisher's exact test. Effect sizes were calculated using Glass's delta, and a significance level of P < 0.05 was applied.
Results: MiR-21 and miR-155 were significantly upregulated in OSCC tissues compared to controls (P < 0.001 for both), with large effect sizes (Glass's δ = 3.78 and 2.63, respectively). No significant association was found between miRNA expression levels and tumor differentiation grade (P > 0.05). HPV was detected in 26.6% of OSCC samples, with HPV-16 being the most frequent subtype. A significant association was observed between HPV positivity and tobacco use (P = 0.002), but not with miRNA expression levels.
Conclusion: The overexpression of miR-21 and miR-155 in OSCC supports their potential as diagnostic biomarkers in oral cancer. While their levels were not significantly associated with tumor grade or HPV status, their consistent elevation in malignant tissues warrants further investigation into their mechanistic role in OSCC pathogenesis and their applicability in biomarker panels for early detection, especially in Latin American populations.
期刊介绍：
It is a journal aimed for research, scientific facts and details covering all specialties of dentistry with a good determination for exploring and sharing the knowledge in the medical and dental fraternity. The scope is therefore huge covering almost all streams of dentistry - starting from original studies, systematic reviews, narrative reviews, very unique case reports. Journal scope is not limited to these subjects and is more wider covering all specialities of dentistry follows: -Preventive and Community dentistry (Dental public health)- Endodontics- Oral and maxillofacial pathology- Oral and maxillofacial radiology- Oral and maxillofacial surgery (also called oral surgery)- Orthodontics and dentofacial orthopedics- Periodontology (also called periodontics)- Pediatric dentistry (also called pedodontics)- Prosthodontics (also called prosthetic dentistry)- Oral medicine- Special needs dentistry (also called special care dentistry)- Oral Biology- Forensic odontology- Geriatric dentistry or Geriodontics- Preventive and Social Medicine (Public health)- Our journal appreciates research articles pertaining with advancement of dentistry, preventive and community dentistry including oral epidemiology, oral health services research, oral health education and promotion, behavioral sciences related to dentistry, dental jurisprudence, ethics and oral health, economics, and quality assessment, recent advances in preventive dentistry and community dentistry.
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