Enas T Abdel-Salam, Zeinab M Anwar, Ahmed Z Ibrahim, Hend M Musatfa
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A Rapid Fluorescence Quenching Assay for Total Levothyroxine Quantification in Pharmaceutical and Supratherapeutic Serum Samples.
A rapid, cost-effective spectrofluorometric method was developed for levothyroxine (T4) detection using fluorescein isothiocyanate (FITC) as a fluorescent probe. The method exploits FITC fluorescence quenching by T4 in Tris/phosphate buffers (pH 7.4),with linear ranges of 8.00 × 10⁻⁶ - 6.40 × 10⁻⁵ mol/L and detection limits of 9.60 × 10⁻⁶ mol/L (Tris) and 8.80 × 10⁻⁶ mol/L (phosphate). Stern-Volmer analysis confirmed dynamic quenching dominated by electrostatic interactions. The method demonstrated 102-104% recovery in spiked human serum and minimal interference from common drugs (Ciprofloxacin, Alendronate) and ions (Fe³⁺, Al³⁺). Compared to HPLC and kinetic methods, this approach offers simplicity, speed, and accessibility for routine pharmaceutical/clinical analysis.
期刊介绍：
Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
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