振动显微切开术的极限。

IF 1.5 4区 生物学 Q4 CELL BIOLOGY
Alexey Klepukov
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引用次数: 0

摘要

Vibratome或振动切片机允许对小鼠大脑进行切片,其厚度从50 μ m开始可靠地确定,但它们对适合切割的样品大小有很强的限制。本文描述了使用公开可用的元件基(来自3D打印机的部件)构建所谓的3D振动切片机,该切片机能够将较大尺寸的大脑切割成薄片,并使用它来研究最小切片厚度的极限可达值。这两个目标都已成功实现。获得最小厚度为30 μm的小鼠小脑片和最小厚度为150 μm的大全牛脑片。揭示了叶片振动频率和进给速度对最小切片厚度影响的关键特征,建立了最小可达厚度与其面积之间的明显相关性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Limits of Vibrating Microtomy.

The Vibratome, or vibrating microtome allows sectioning of the mouse brain with a reliably established thickness from 50 µm, but they have a strong limitation on the size of the sample suitable for cutting. Herein is described the construction with publicly available element base (from parts for a 3D printer) a so-called 3D-vibrating microtome capable of cutting larger size brain into thin sections, and use it to investigate the limit-attainable values of the minimum slice thickness. Both of these goals have been successfully achieved. Both small mouse slices with a minimum thickness of 30 μm and large whole calf brain slices with a minimum thickness of 150 μm were obtained. Critical features of the effect of blade vibration frequency and sample feed rate on the minimum slice thickness were revealed, In the same way, a clear correlation was established between the minimum achievable thickness and its area.

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来源期刊
CiteScore
6.00
自引率
0.00%
发文量
32
审稿时长
1 months
期刊介绍: Journal of Histochemistry & Cytochemistry (JHC) has been a pre-eminent cell biology journal for over 50 years. Published monthly, JHC offers primary research articles, timely reviews, editorials, and perspectives on the structure and function of cells, tissues, and organs, as well as mechanisms of development, differentiation, and disease. JHC also publishes new developments in microscopy and imaging, especially where imaging techniques complement current genetic, molecular and biochemical investigations of cell and tissue function. JHC offers generous space for articles and recognizing the value of images that reveal molecular, cellular and tissue organization, offers free color to all authors.
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